AB202125

Anti-FAM134C antibody - C-terminal

Name: Anti-FAM134C antibody - C-terminal (ab202125) | Abcam
Brand: Abcam Limited
SKU: AB202125
Rating: 3 (2 reviews)

(2 Reviews)

(2 Publications)

Rabbit Polyclonal FAM134C antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RETREG3 aa 400-500.

View Alternative Names

FAM134C, RETREG3, Reticulophagy regulator 3

2 Images

Supplier Data

Immunoprecipitation - Anti-FAM134C antibody - C-terminal (AB202125)

Lane 1 : Detection of FAM134C in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 0.5 or 1.0 mg per IP reaction, 20% of IP loaded) using ab202125 at 6 μg per reaction, and at 1 μg/ml for subsequent Western blot detection.
Lane 2 : controle IgG
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-FAM134C antibody - C-terminal (ab202125)

Predicted band size: 51 kDa

true

Exposure time: 10s

Supplier Data

Western blot - Anti-FAM134C antibody - C-terminal (AB202125)

All lanes:

Western blot - Anti-FAM134C antibody - C-terminal (ab202125) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 51 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human RETREG3 aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

Q86VR2

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAM134C also known as family with sequence similarity 134 member C is a protein involved in autophagy processes. It has a molecular mass of approximately 54 kDa. This protein localizes mainly within the endoplasmic reticulum (ER) membrane and it is expressed in several tissues including the brain and liver. FAM134C plays an essential role in regulating the ER shape and maintaining cellular homeostasis.

Biological function summary

The protein participates in the process of selective autophagy particularly ER-phagy which is the degradation of ER fragments. This function indicates that it is part of a broader complex that regulates the destruction of damaged or excessive organelles which is important for neuron and liver cell function. FAM134C interacts with LC3 proteins during these processes acting as a receptor that identifies ER regions targeted for degradation.

Pathways

Researchers recognize FAM134C's involvement in the cellular pathway of autophagy and apoptosis. The protein operates within these pathways by interacting with other autophagy-related proteins such as ATG8 family members which aid in forming autophagosomes. These pathways influence the balance between cell survival and cell death showcasing the protein's role in maintaining cellular health and function.

Studies indicate a connection of FAM134C to neurodegenerative diseases and cancer. Dysregulation of its function can lead to the accumulation of damaged organelles contributing to neurodegenerative conditions like Alzheimer's disease. Moreover aberrant expression levels of FAM134C have been noted in certain cancer types suggesting its involvement in cancer progression. Disorders involving this protein often also implicate the LC3 protein reinforcing its participation in pathways related to pathological conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Endoplasmic reticulum (ER)-anchored autophagy regulator which exists in an inactive state under basal conditions but is activated following cellular stress (PubMed : 34338405). When activated, induces ER fragmentation and mediates ER delivery into lysosomes through sequestration into autophagosomes via interaction with ATG8 family proteins (PubMed : 34338405). Promotes ER membrane curvature and ER tubulation required for subsequent ER fragmentation and engulfment into autophagosomes (PubMed : 33826365). Required for collagen quality control in a LIR motif-dependent manner (By similarity). Mediates NRF1-enhanced neurite outgrowth (PubMed : 26040720).

See full target information RETREG3

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:2698 PubMed37164963

2023

Viral subversion of selective autophagy is critical for biogenesis of virus replication organelles.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Lan,Sophie Wilhelmina van Leur,Julia Ayano Fernando,Ho Him Wong,Martin Kampmann,Lewis Siu,Jingshu Zhang,Mingyuan Li,John M Nicholls,Sumana Sanyal

European journal of medical research 27:205 PubMed36253873

2022

Construction of mRNA prognosis signature associated with differentially expressed genes in early stage of stomach adenocarcinomas based on TCGA and GEO datasets.

Applications

Unspecified application

Species

Unspecified reactive species

Fuquan Jiang,Haiguan Lin,Hongfeng Yan,Xiaomin Sun,Jianwu Yang,Manku Dong

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAM134C antibody - C-terminal

Immunoprecipitation - Anti-FAM134C antibody - C-terminal (AB202125)

Western blot - Anti-FAM134C antibody - C-terminal (AB202125)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise