AB214177

Anti-FAM149B1 antibody

Name: Anti-FAM149B1 antibody (ab214177) | Abcam
Brand: Abcam Limited
SKU: AB214177
Price: 510 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal FAM149B1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FAM149B1 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KIAA0974, FAM149B1, Primary cilium assembly protein FAM149B1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM149B1 antibody (AB214177)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM149B1 antibody (AB214177)

Immunohistochemical analysis of formalin-fixed and paraffin embedded human spleen tissue labeling FAM149B1 using ab214177 at 1/200, followed by secondary detection and DAB staining.

Supplier Data

Western blot - Anti-FAM149B1 antibody (AB214177)

All lanes:

Western blot - Anti-FAM149B1 antibody (ab214177) at 1/300 dilution

All lanes:

mouse bone lysate

Secondary

All lanes:

Goat anti-rabbit IgG (H+L), HRP conjugated at 1/10000 dilution

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human FAM149B1 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q96BN6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAM149B1 also known as Family with Sequence Similarity 149 Member B1 is a protein with a molecular weight of approximately 56 kDa. This protein is mainly expressed in the cytoplasm and shows variable levels of presence in different tissues with significant observations in the brain and liver. FAM149B1 is involved in cellular signaling processes although its precise mechanical roles in cellular activities are still under investigation.

Biological function summary

FAM149B1 functions as part of a multiprotein complex and is involved in regulating cellular metabolism and signaling pathways. This protein has interactions that suggest roles in modulating metabolic processes and possibly cell cycle regulation. FAM149B1 also shows potential involvement in maintaining cellular homeostasis suggesting a broader impact on various cellular functions.

Pathways

FAM149B1 engages in the mTOR signaling and molecular chaperone systems. These pathways are critical for cell growth proliferation and response to stress. In mTOR signaling FAM149B1 potentially interacts with proteins like mTOR itself and Raptor which are essential for the pathway's regulation and function. By contributing to these pathways FAM149B1 plays a role in cellular responses to nutrient availability and stress.

FAM149B1 shows associations with metabolic disorders such as insulin resistance and possibly neurodegenerative diseases. These associations might relate to its roles in mTOR signaling and metabolic regulation. Disorders involving FAM149B1 could involve alterations in proteins like mTOR and Hsp90 where imbalances can lead to disease states. The protein's involvement in these diseases highlights its significance as a potential target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Involved in the localization of proteins to the cilium and cilium assembly. Indirectly regulates the signaling functions of the cilium, being required for normal SHH/smoothened signaling and proper development.

See full target information FAM149B1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1500478 PubMed39687617

2024

PCDHGA10 as a potential prognostic biomarker and correlated with immune infiltration in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyang Zhong,Zhuoqun Yu,Qianqian Wu,Bing Lu,PingPing Sun,Xiaojing Zhang,Lei Yang,Han Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAM149B1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM149B1 antibody (AB214177)

Western blot - Anti-FAM149B1 antibody (AB214177)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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