AB74333

Anti-FAM175B antibody

Name: Anti-FAM175B antibody (ab74333) | Abcam
Brand: Abcam Limited
SKU: AB74333
Price: 520 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal FAM175B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ABRAXAS2.

View Alternative Names

ABRO1, FAM175B, KIAA0157, ABRAXAS2, BRISC complex subunit Abraxas 2, Abraxas brother protein 1, Protein FAM175B

2 Images

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Immunoprecipitation - Anti-FAM175B antibody (AB74333)

ab74333 at 1 ug/ml (used for IP at 3 ug/lysate).
Lane 1 : Whole HeLa cell lysate at 1 mg for IP (20% of IP loaded).
Lane 2 : control IgG.
Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-FAM175B antibody (ab74333)

Predicted band size: 47 kDa

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Western blot - Anti-FAM175B antibody (AB74333)

Detection : Chemiluminescence with exposure times of 10 seconds

All lanes:

Western blot - Anti-FAM175B antibody (ab74333) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK293T whole cell lysate at 50 µg

Predicted band size: 47 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ABRAXAS2. The exact immunogen used to generate this antibody is proprietary information.

Q15018

Specificity

The antibody recognises a region between residue 350 and 390 of human FAM175B using the numbering given in entry NP_115558.3 (GeneID 23172).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was affinity purified using an epitope specific to FAM175B immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAM175B also known as ABRAXAS2 is a protein with a molecular weight of approximately 42 kDa. It plays an important role in DNA repair processes. Expression of FAM175B is observed in various tissues with notable presence in the nucleus due to its involvement in DNA damage response. The protein has a significant presence in actively dividing cells where maintenance of genomic integrity is essential.

Biological function summary

FAM175B is part of the BRCA1 A complex which is an important player in homologous recombination repair. This complex helps in recognizing and repairing double-strand breaks in DNA a critical function for preventing genomic instability. FAM175B directly interacts with other components of this complex such as BRCA1 and RAP80 ensuring precise repair of DNA. The protein acts as a scaffold coordinating protein-protein interactions necessary for complex formation and function.

Pathways

FAM175B is an indispensable component of the DNA damage response and repair pathway. This pathway is paramount for cellular resistance to DNA-damaging agents. FAM175B functions alongside BRCA1 and other associated proteins like RAD51 to facilitate repair processes. It links the recognition of DNA lesions with the execution of DNA repair playing a pivotal role in maintaining cellular health.

FAM175B has a significant association with breast cancer susceptibility. Mutations or malfunctions within the BRCA1 A complex where FAM175B operates can escalate risks for developing breast cancer. Additionally FAM175B is indirectly connected to ovarian cancer through its interactions with BRCA1. Its dysfunction in these pathways contributes to impaired DNA repair leading to tumorigenesis in these tissues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked polyubiquitin, leaving the last ubiquitin chain attached to its substrates (PubMed : 19214193, PubMed : 20032457, PubMed : 20656690, PubMed : 24075985). May act as a central scaffold protein that assembles the various components of the BRISC complex and retains them in the cytoplasm (PubMed : 20656690). Plays a role in regulating the onset of apoptosis via its role in modulating 'Lys-63'-linked ubiquitination of target proteins (By similarity). Required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed : 26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activities by enhancing its stability and cell surface expression (PubMed : 24075985, PubMed : 26344097). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed : 24075985). Required for normal induction of p53/TP53 in response to DNA damage (PubMed : 25283148). Independent of the BRISC complex, promotes interaction between USP7 and p53/TP53, and thereby promotes deubiquitination of p53/TP53, preventing its degradation and resulting in increased p53/TP53-mediated transcription regulation and p53/TP53-dependent apoptosis in response to DNA damage (PubMed : 25283148).

See full target information ABRAXAS2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Leukemia 34:404-415 PubMed31576005

2019

Functional characterization of BRCC3 mutations in acute myeloid leukemia with t(8;21)(q22;q22.1).

Applications

Unspecified application

Species

Unspecified reactive species

Tatjana Meyer,Nikolaus Jahn,Stefanie Lindner,Linda Röhner,Anna Dolnik,Daniela Weber,Annika Scheffold,Simon Köpff,Peter Paschka,Verena I Gaidzik,Dirk Heckl,Sebastian Wiese,Benjamin L Ebert,Hartmut Döhner,Lars Bullinger,Konstanze Döhner,Jan Krönke

Molecular cell 75:483-497.e9 PubMed31253574

2019

Structural Basis of BRCC36 Function in DNA Repair and Immune Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Julius Rabl,Richard D Bunker,Andreas D Schenk,Simone Cavadini,Mark E Gill,Wassim Abdulrahman,Amparo Andrés-Pons,Martijn S Luijsterburg,Adel F M Ibrahim,Emma Branigan,Jacob D Aguirre,Aimee H Marceau,Claire Guérillon,Tewis Bouwmeester,Ulrich Hassiepen,Antoine H F M Peters,Martin Renatus,Laurent Gelman,Seth M Rubin,Niels Mailand,Haico van Attikum,Ronald T Hay,Nicolas H Thomä

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAM175B antibody

Immunoprecipitation - Anti-FAM175B antibody (AB74333)

Western blot - Anti-FAM175B antibody (AB74333)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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