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AB224234

Anti-FAM198B antibody

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(1 Publication)

Rabbit Polyclonal FAM198B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GASK1B aa 1-200.

View Alternative Names

C4orf18, ENED, FAM198B, AD021, UNQ2512/PRO6001, GASK1B, Golgi-associated kinase 1B, Expressed in nerve and epithelium during development, Protein FAM198B

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM198B antibody (AB224234)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM198B antibody (AB224234)

Immunohistochemical analysis of human adrenal glandctissue labeling FAM198B in the cytoplasm of glandular cells with ab224234 at 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-FAM198B antibody (AB224234)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FAM198B antibody (AB224234)

Immunofluorescent analysis of HepG2 (human liver hepatocellular carcinoma cell line) whole cells labeling FAM198B (green) in nucleus, nucleoli fibrillar center and the Golgi apparatus with ab224234 at 2 μg/ml.

Western blot - Anti-FAM198B antibody (AB224234)
  • WB

Supplier Data

Western blot - Anti-FAM198B antibody (AB224234)

All lanes:

Western blot - Anti-FAM198B antibody (ab224234) at 0.4 µg/mL

Lane 1:

Control (vector only transfected) - HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

FAM198B over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human GASK1B aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q6UWH4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FAM198B protein also known as Family with Sequence Similarity 198 Member B is an interesting biological target with yet undisclosed precise functions. It has a mass of approximately 64 kDa. FAM198B is expressed in various types of tissue notably in brain and skeletal muscle tissues. While the protein's exact mechanical role remains incompletely characterized initial research highlights its potential regulatory activities in cellular processes due to its expression patterns.
Biological function summary

FAM198B might influence cellular pathways involving muscle function and neurological systems. Although it is not currently known to be part of a protein complex its expression suggests involvement in cellular growth or differentiation processes. Its behaviors upon overexpression or suppression in studies suggest it may impact cell cycle regulation potentially by interacting with other regulatory proteins or genetic regions.

Pathways

FAM198B may intersect with the MAPK signaling pathway known for its role in regulating cellular activities such as proliferation differentiation and response to external stress signals. It may also show a link to the PI3K-Akt signaling pathway impacting cell survival and metabolism. These pathways involve a multitude of proteins including kinases and phosphatases pointing towards a process where FAM198B could modulate certain steps within these pathways either directly or through secondary messengers.

FAM198B has potential associations with neurodegenerative diseases and muscle wasting disorders. Its expression profile in brain and muscle tissues connects it to clinical conditions where these tissues are affected such as Alzheimer's disease or muscular dystrophies. Studies are also probing its interaction with proteins like Tau linked to neurodegeneration which could further elucidate its role in these diseases. Understanding FAM198B’s exact impacts offers new perspectives on therapeutic strategies targeting these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information GASK1B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 3:100541 PubMed35732148

2022

Phosphodiesterase type 5 inhibitors enhance chemotherapy in preclinical models of esophageal adenocarcinoma by targeting cancer-associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin P Sharpe,Annette Hayden,Antigoni Manousopoulou,Andrew Cowie,Robert C Walker,Jack Harrington,Fereshteh Izadi,Stella P Breininger,Jane Gibson,Oliver Pickering,Eleanor Jaynes,Ewan Kyle,John H Saunders,Simon L Parsons,Alison A Ritchie,Philip A Clarke,Pamela Collier,Nigel P Mongan,David O Bates,Kiren Yacqub-Usman,Spiros D Garbis,Zoë Walters,Matthew Rose-Zerilli,Anna M Grabowska,Timothy J Underwood
View all publications
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Product promise

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