Rabbit Recombinant Monoclonal FAM208A antibody. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-FAM208A antibody [EPR28577-82] (AB322400), expandable thumbnail](https://content.abcam.com/products/images/fam208a-antibody-epr28577-82-ab322400--western-blot-img440097.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAM208A antibody [EPR28577-82] (AB322400)")
![Western blot - Anti-FAM208A antibody [EPR28577-82] (AB322400), expandable thumbnail](https://content.abcam.com/products/images/fam208a-antibody-epr28577-82-ab322400--western-blot-img440098.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAM208A antibody [EPR28577-82] (AB322400)")
![Western blot - Anti-FAM208A antibody [EPR28577-82] (AB322400), expandable thumbnail](https://content.abcam.com/products/images/fam208a-antibody-epr28577-82-ab322400--western-blot-img440099.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAM208A antibody [EPR28577-82] (AB322400)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | ICC/IF | |
|---|---|---|---|
| Human | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Function
Component of the HUSH complex, a multiprotein complex that mediates epigenetic repression (PubMed:26022416, PubMed:28581500). The HUSH complex is recruited to genomic loci rich in H3K9me3 and is required to maintain transcriptional silencing by promoting recruitment of SETDB1, a histone methyltransferase that mediates further deposition of H3K9me3, as well as MORC2 (PubMed:26022416, PubMed:28581500). Also represses L1 retrotransposons in collaboration with MORC2 and, probably, SETDB1, the silencing is dependent of repressive epigenetic modifications, such as H3K9me3 mark. Silencing events often occur within introns of transcriptionally active genes, and lead to the down-regulation of host gene expression (PubMed:29211708). The HUSH complex is also involved in the silencing of unintegrated retroviral DNA by being recruited by ZNF638: some part of the retroviral DNA formed immediately after infection remains unintegrated in the host genome and is transcriptionally repressed (PubMed:30487602). Plays a crucial role in early embryonic development (By similarity). Involved in the organization of spindle poles and spindle apparatus assembly during zygotic division (By similarity). Plays an important role in maintaining epiblast fitness or potency (By similarity).
Alternative names
C3orf63, FAM208A, KIAA1105, TASOR, Protein TASOR, CTCL tumor antigen se89-1, Retinoblastoma-associated protein RAP140, Transgene activation suppressor protein
Rabbit Recombinant Monoclonal FAM208A antibody. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR28577-82
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-FAM208A antibody [EPR28577-82] (ab322400), expandable thumbnail](https://content.abcam.com/products/images/fam208a-antibody-epr28577-82-ab322400--western-blot-img440097.jpg "Western blot - Anti-FAM208A antibody [EPR28577-82] (ab322400)")
Western blot - Anti-FAM208A antibody [EPR28577-82] (ab322400)
FAM208A Western blot staining using rabbit Anti-FAM208A antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: skeletal muscle
The bands beneath the target band (190 kDa) are likely to be degraded target fragments.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-FAM208A antibody [EPR28577-82] (ab322400) at 1/1000 dilution
Lane 1: Human testis tissue lysate at 20 µg
Lane 2: Human skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 190, 250 kDa, 124 kDa
Exposure time: 180s
![Western blot - Anti-FAM208A antibody [EPR28577-82] (ab322400), expandable thumbnail](https://content.abcam.com/products/images/fam208a-antibody-epr28577-82-ab322400--western-blot-img440098.jpg "Western blot - Anti-FAM208A antibody [EPR28577-82] (ab322400)")
Western blot - Anti-FAM208A antibody [EPR28577-82] (ab322400)
FAM208A Western blot staining using rabbit Anti-FAM208A antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
In lane 1-3, the lysate was stored at -80℃ prior to Western Blotting. In lanes 4, To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
The bands beneath the target band (190 kDa) are likely to be degraded target fragments.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
Exposure time: Lane1-3:92 seconds, lane4:180 seconds
All lanes: Western blot - Anti-FAM208A antibody [EPR28577-82] (ab322400) at 1/1000 dilution
Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole lysate at 20 µg
Lane 2: DLD-1 (human colorectal adenocarcinoma epithelial cell) whole lysate at 20 µg
Lane 3: SW620 (human colorectal adenocarcinoma epithelial cell) whole lysate at 20 µg
Lane 4: 293T (human embryonic kidney epithelial cell) whole lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Observed band size: 192, 250 kDa, 124 kDa
![Western blot - Anti-FAM208A antibody [EPR28577-82] (ab322400), expandable thumbnail](https://content.abcam.com/products/images/fam208a-antibody-epr28577-82-ab322400--western-blot-img440099.jpg "Western blot - Anti-FAM208A antibody [EPR28577-82] (ab322400)")
Western blot - Anti-FAM208A antibody [EPR28577-82] (ab322400)
FAM208A Western blot staining using rabbit Anti-FAM208A antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The bands beneath the target band (190 kDa) are likely to be degraded target fragments.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-FAM208A antibody [EPR28577-82] (ab322400) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: HeLa transfected with siRNA specifically targeting FAM208A whole cell lysate at 20 µg
Lane 3: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 190, 250 kDa, 124 kDa
Exposure time: 92s
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