Anti-FAM208A antibody [EPR28577-82] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-FAM208A antibody [EPR28577-82] - BSA and Azide free (AB324845)

This data was developed using ab322400, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : skeletal muscle

The bands beneath the target band (190 kDa) are likely to be degraded target fragments.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-FAM208A antibody [EPR28577-82] (<a href='/en-us/products/primary-antibodies/fam208a-antibody-epr28577-82-ab322400'>ab322400</a>) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 190 kDa,250 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-FAM208A antibody [EPR28577-82] - BSA and Azide free (AB324845)

This data was developed using ab322400, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In lane 1-3, the lysate was stored at -80℃ prior to Western Blotting. In lanes 4, To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The bands beneath the target band (190 kDa) are likely to be degraded target fragments.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

Exposure time : Lane1-3 : 92 seconds, lane4 : 180 seconds

All lanes:

Western blot - Anti-FAM208A antibody [EPR28577-82] (<a href='/en-us/products/primary-antibodies/fam208a-antibody-epr28577-82-ab322400'>ab322400</a>) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole lysate at 20 µg

Lane 2:

DLD-1 (human colorectal adenocarcinoma epithelial cell) whole lysate at 20 µg

Lane 3:

SW620 (human colorectal adenocarcinoma epithelial cell) whole lysate at 20 µg

Lane 4:

293T (human embryonic kidney epithelial cell) whole lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Observed band size: 192 kDa,250 kDa,124 kDa

false

• WB

Supplier Data

Western blot - Anti-FAM208A antibody [EPR28577-82] - BSA and Azide free (AB324845)

This data was developed using ab322400, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The bands beneath the target band (190 kDa) are likely to be degraded target fragments.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-FAM208A antibody [EPR28577-82] (<a href='/en-us/products/primary-antibodies/fam208a-antibody-epr28577-82-ab322400'>ab322400</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting FAM208A whole cell lysate at 20 µg

Lane 3:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 190 kDa,250 kDa,124 kDa

false

Exposure time: 92s