Rabbit Polyclonal FAM20C antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAM20C aa 200-550.
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
WB | ICC/IF | IHC-P | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted |
Rat | Predicted | Predicted | Predicted |
Monkey | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Monkey | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Monkey | Dilution info - | Notes - |
Golgi serine/threonine protein kinase that phosphorylates secretory pathway proteins within Ser-x-Glu/pSer motifs and plays a key role in biomineralization of bones and teeth (PubMed:22582013, PubMed:23754375, PubMed:25789606). Constitutes the main protein kinase for extracellular proteins, generating the majority of the extracellular phosphoproteome (PubMed:26091039). Mainly phosphorylates proteins within the Ser-x-Glu/pSer motif, but also displays a broader substrate specificity (PubMed:26091039). Phosphorylates ERO1A, enhancing its activity which is required to maintain endoplasmic reticulum redox homeostasis and for oxidative protein folding (PubMed:29858230, PubMed:34349020). During endoplasmic reticulum stress, phosphorylates P4HB/PDIA1 which induces a functional switch, causing P4HB to change from an oxidoreductase to a molecular chaperone (PubMed:32149426). This is critical to maintain ER proteostasis and reduce cell death under ER stress (PubMed:32149426). Phosphorylation of P4HB also promotes its interaction with ERN1, leading to reduced activity of ERN1, a key sensor for the endoplasmic reticulum unfolded protein response (PubMed:32149426). Required for osteoblast differentiation and mineralization (PubMed:34349020). Phosphorylates casein as well as a number of proteins involved in biomineralization such as AMELX, AMTN, ENAM and SPP1/OPN (PubMed:22582013, PubMed:25789606, PubMed:34349020). In addition to its role in biomineralization, also plays a role in lipid homeostasis, wound healing and cell migration and adhesion (PubMed:26091039).
DMP4, FAM20C, Extracellular serine/threonine protein kinase FAM20C, Dentin matrix protein 4, Golgi casein kinase, Golgi-enriched fraction casein kinase, DMP-4, GEF-CK
Rabbit Polyclonal FAM20C antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAM20C aa 200-550.
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft labeling FAM20C with ab154740 at 1/100 dilution.
7.5% SDS PAGE
All lanes: Western blot - Anti-FAM20C antibody (ab154740) at 1/500 dilution
All lanes: Molt4 whole cell lysate at 30 µg
Predicted band size: 66 kDa
Immunofluorescent analysis of methanol-fixed Hep3B cells labeling FAM20C with ab154740 at 1/200 dilution. Lower panel was co-stained with Hoechst 33342.
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