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AB154740

Anti-FAM20C antibody

1

(1 Review)

|

(8 Publications)

Rabbit Polyclonal FAM20C antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAM20C aa 200-550.

View Alternative Names

DMP4, FAM20C, Extracellular serine/threonine protein kinase FAM20C, Dentin matrix protein 4, Golgi casein kinase, Golgi-enriched fraction casein kinase, DMP-4, GEF-CK

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-FAM20C antibody (AB154740)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FAM20C antibody (AB154740)

Immunofluorescent analysis of methanol-fixed Hep3B cells labeling FAM20C with ab154740 at 1/200 dilution. Lower panel was co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM20C antibody (AB154740)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM20C antibody (AB154740)

Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft labeling FAM20C with ab154740 at 1/100 dilution.

Western blot - Anti-FAM20C antibody (AB154740)
  • WB

Unknown

Western blot - Anti-FAM20C antibody (AB154740)

7.5% SDS PAGE

All lanes:

Western blot - Anti-FAM20C antibody (ab154740) at 1/500 dilution

All lanes:

Molt4 whole cell lysate at 30 µg

Predicted band size: 66 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FAM20C aa 200-550. The exact immunogen used to generate this antibody is proprietary information.

Q8IXL6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Golgi serine/threonine protein kinase that phosphorylates secretory pathway proteins within Ser-x-Glu/pSer motifs and plays a key role in biomineralization of bones and teeth (PubMed : 22582013, PubMed : 23754375, PubMed : 25789606). Constitutes the main protein kinase for extracellular proteins, generating the majority of the extracellular phosphoproteome (PubMed : 26091039). Mainly phosphorylates proteins within the Ser-x-Glu/pSer motif, but also displays a broader substrate specificity (PubMed : 26091039). Phosphorylates ERO1A, enhancing its activity which is required to maintain endoplasmic reticulum redox homeostasis and for oxidative protein folding (PubMed : 29858230, PubMed : 34349020). During endoplasmic reticulum stress, phosphorylates P4HB/PDIA1 which induces a functional switch, causing P4HB to change from an oxidoreductase to a molecular chaperone (PubMed : 32149426). This is critical to maintain ER proteostasis and reduce cell death under ER stress (PubMed : 32149426). Phosphorylation of P4HB also promotes its interaction with ERN1, leading to reduced activity of ERN1, a key sensor for the endoplasmic reticulum unfolded protein response (PubMed : 32149426). Required for osteoblast differentiation and mineralization (PubMed : 34349020). Phosphorylates casein as well as a number of proteins involved in biomineralization such as AMELX, AMTN, ENAM and SPP1/OPN (PubMed : 22582013, PubMed : 25789606, PubMed : 34349020). In addition to its role in biomineralization, also plays a role in lipid homeostasis, wound healing and cell migration and adhesion (PubMed : 26091039).
See full target information FAM20C
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 12:e70126 PubMed39532808

2024

Lack of adipocyte FAM20C improves whole body glucose homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Deng,Yanshan Huang,Feifei Zhao,Puxin Chen,Xiaohong Huang

Nature communications 14:1028 PubMed36823172

2023

Spatial transcriptomics reveals niche-specific enrichment and vulnerabilities of radial glial stem-like cells in malignant gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Yanming Ren,Zongyao Huang,Lingling Zhou,Peng Xiao,Junwei Song,Ping He,Chuanxing Xie,Ran Zhou,Menghan Li,Xiangqun Dong,Qing Mao,Chao You,Jianguo Xu,Yanhui Liu,Zhigang Lan,Tiejun Zhang,Qi Gan,Yuan Yang,Tengyun Chen,Bowen Huang,Xiang Yang,Anqi Xiao,Yun Ou,Zhengzheng Su,Lu Chen,Yan Zhang,Yan Ju,Yuekang Zhang,Yuan Wang

Frontiers in genetics 12:757014 PubMed34970298

2021

Fam20C Overexpression Predicts Poor Outcomes and is a Diagnostic Biomarker in Lower-Grade Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Feng,Jinping Zhou,Lin Zhao,Xinpeng Wang,Danyu Ma,Baoqing Xu,Feilai Xie,Xingfeng Qi,Gang Chen,Hu Zhao,Junxin Wu

OncoTargets and therapy 13:11755-11768 PubMed33239887

2020

Secretory Pathway Kinase , a Marker for Glioma Invasion and Malignancy, Predicts Poor Prognosis of Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Shaonan Du,Shu Guan,Chen Zhu,Qing Guo,Jingyuan Cao,Gefei Guan,Wen Cheng,Peng Cheng,Anhua Wu

Genes 11: PubMed32093234

2020

Two Novel Variants in A Family with Raine Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Araceli Hernández-Zavala,Fernando Cortés-Camacho,Icela Palma Lara,Ricardo Godinez-Aguilar,Ana María Espinosa-García,Javier Pérez-Durán,Patricia Villanueva-Ocampo,Carlos Ugarte-Briones,Carlos Alberto Serrano-Bello,Paula Sanchez-Santiago,José Bonilla-Delgado,Marco Antonio Yañez-López,Georgina Victoria-Acosta,Adolfo López-Ornelas,Patricia García Alonso-Themann,José Moreno,Carmen Palacios-Reyes

The FEBS journal 285:4674-4683 PubMed30387551

2018

The Golgi 'casein kinase' Fam20C is a genuine 'phosvitin kinase' and phosphorylates polyserine stretches devoid of the canonical consensus.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgio Cozza,Enrico Moro,Miles Black,Oriano Marin,Mauro Salvi,Andrea Venerando,Vincent S Tagliabracci,Lorenzo A Pinna

Endocrinology 158:1130-1139 PubMed28324013

2017

Acute Parathyroid Hormone Injection Increases C-Terminal but Not Intact Fibroblast Growth Factor 23 Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa M Knab,Braden Corbin,Olena Andrukhova,Julia M Hum,Pu Ni,Seham Rabadi,Akira Maeda,Kenneth E White,Reinhold G Erben,Harald Jüppner,Marta Christov

The FEBS journal 284:1246-1257 PubMed28236661

2017

Fam20C is under the control of sphingolipid signaling in human cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgio Cozza,Mauro Salvi,Vincent S Tagliabracci,Lorenzo A Pinna
View all publications
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Product promise

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