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Rabbit Polyclonal FAM20C antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAM20C aa 200-550.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM20C antibody (AB154740), expandable thumbnail
  • Western blot - Anti-FAM20C antibody (AB154740), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-FAM20C antibody (AB154740), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human FAM20C aa 200-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IXL6

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/3000.00000
Notes

-

Predicted
Predicted

Species
Mouse, Rat, Monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/1000.00000
Notes

-

Predicted
Predicted

Species
Mouse, Rat, Monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100.00000 - 1/1000.00000
Notes

Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Predicted
Predicted

Species
Mouse, Rat, Monkey
Dilution info
-
Notes

-

Target data

Function

Golgi serine/threonine protein kinase that phosphorylates secretory pathway proteins within Ser-x-Glu/pSer motifs and plays a key role in biomineralization of bones and teeth (PubMed:22582013, PubMed:23754375, PubMed:25789606). Constitutes the main protein kinase for extracellular proteins, generating the majority of the extracellular phosphoproteome (PubMed:26091039). Mainly phosphorylates proteins within the Ser-x-Glu/pSer motif, but also displays a broader substrate specificity (PubMed:26091039). Phosphorylates ERO1A, enhancing its activity which is required to maintain endoplasmic reticulum redox homeostasis and for oxidative protein folding (PubMed:29858230, PubMed:34349020). During endoplasmic reticulum stress, phosphorylates P4HB/PDIA1 which induces a functional switch, causing P4HB to change from an oxidoreductase to a molecular chaperone (PubMed:32149426). This is critical to maintain ER proteostasis and reduce cell death under ER stress (PubMed:32149426). Phosphorylation of P4HB also promotes its interaction with ERN1, leading to reduced activity of ERN1, a key sensor for the endoplasmic reticulum unfolded protein response (PubMed:32149426). Required for osteoblast differentiation and mineralization (PubMed:34349020). Phosphorylates casein as well as a number of proteins involved in biomineralization such as AMELX, AMTN, ENAM and SPP1/OPN (PubMed:22582013, PubMed:25789606, PubMed:34349020). In addition to its role in biomineralization, also plays a role in lipid homeostasis, wound healing and cell migration and adhesion (PubMed:26091039).

Alternative names

Recommended products

Rabbit Polyclonal FAM20C antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAM20C aa 200-550.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Fragment Protein within Human FAM20C aa 200-550. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IXL6
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM20C antibody (ab154740), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM20C antibody (ab154740)

    Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft labeling FAM20C with ab154740 at 1/100 dilution.

  • Western blot - Anti-FAM20C antibody (ab154740), expandable thumbnail

    Western blot - Anti-FAM20C antibody (ab154740)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-FAM20C antibody (ab154740) at 1/500 dilution

    All lanes: Molt4 whole cell lysate at 30 µg

    Predicted band size: 66 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-FAM20C antibody (ab154740), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-FAM20C antibody (ab154740)

    Immunofluorescent analysis of methanol-fixed Hep3B cells labeling FAM20C with ab154740 at 1/200 dilution. Lower panel was co-stained with Hoechst 33342.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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