Rabbit Polyclonal FAM3C/ILEI antibody. Suitable for WB, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 10 publications.
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Publications
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- International journal of molecular sciences 23:2022CD44+ and CD133+ Non-Small Cell Lung Cancer Cells Exhibit DNA Damage Response Pathways and Dormant Polyploid Giant Cancer Cell Enrichment Relating to Their p53 Status.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMargarita Pustovalova,Taisia Blokhina,Lina Alhaddad,Anna Chigasova,Roman Chuprov-Netochin,Alexander Veviorskiy,Gleb Filkov,Andreyan N Osipov,Sergey LeonovPubMed 35563313
- Journal of translational medicine 20:542022The ILEI/LIFR complex induces EMT via the Akt and ERK pathways in renal interstitial fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJieqing Zhou,Hong Jiang,Hongkun Jiang,Yan Fan,Jing Zhang,Xiaoxue Ma,Xuewei Yang,Yu Sun,Xing ZhaoPubMed 35093095
- Journal of cellular and molecular medicine 23:3464-34752019FAM3C-YY1 axis is essential for TGFβ-promoted proliferation and migration of human breast cancer MDA-MB-231 cells via the activation of HSF1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeili Yang,Biaoqi Feng,Yuhong Meng,Junpei Wang,Bin Geng,Qinghua Cui,Hongquan Zhang,Yang Yang,Jichun YangPubMed 30887707
- Oncogene 38:3794-38112019TGFβ promotes breast cancer stem cell self-renewal through an ILEI/LIFR signaling axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlec N Woosley et. al.PubMed 30692635
- The Journal of biological chemistry 293:11401-114142018Interleukin-like EMT inducer (ILEI) promotes melanoma invasiveness and is transcriptionally up-regulated by upstream stimulatory factor-1 (USF-1).Applications:Unspecified applicationReactive species:Unspecified reactive speciesKen Noguchi et. al.PubMed 29871931
- Cell biochemistry and function 36:46-552018ILEI is an important intermediate participating in the formation of TGF-β1-induced renal tubular EMT.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXing Zhao,Gang Luo,Yan Fan,Xiaoxue Ma,Jieqing Zhou,Hong JiangPubMed 29336056
- Oncotarget 8:106038-1060492017FAM3C activates HSF1 to suppress hepatic gluconeogenesis and attenuate hyperglycemia of type 1 diabetic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenzhen Chen et. al.PubMed 29285313
- PloS one 12:e01778302017Interleukin-like EMT inducer regulates partial phenotype switching in MITF-low melanoma cell lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKen Noguchi,Annamarie C Dalton,Breege V Howley,Buckley J McCall,Akihiro Yoshida,J Alan Diehl,Philip H HowePubMed 28545079
- IUBMB life 69:16-212016The ubiquitin ligase UBE4A inhibits prostate cancer progression by targeting interleukin-like EMT inducer (ILEI).Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanan Sun,Xiaopeng Jia,Qiang Gao,Xing Liu,Lianguo HouPubMed 27862841
- Histopathology 65:527-382014ILEI: a novel marker for epithelial-mesenchymal transition and poor prognosis in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhao-Hua Gao,Chong Lu,Zhen-Ning Wang,Yong-Xi Song,Jin-Liang Zhu,Peng Gao,Jing-Xu Sun,Xiao-Wan Chen,Mei-Xian Wang,Yu-Lan Dong,Hui-Mian XuPubMed 24738665
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Tested | Expected |
| Chimpanzee | Predicted | Predicted |
| Opossum | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Opossum | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5.00000-10.00000 µg/mL | Notes - |
Species Human | Dilution info 5.00000-10.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Opossum | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
May be involved in retinal laminar formation. Promotes epithelial to mesenchymal transition.
Alternative names
ILEI, GS3786, FAM3C, Protein FAM3C, Interleukin-like EMT inducer
Recommended products
Rabbit Polyclonal FAM3C/ILEI antibody. Suitable for WB, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 10 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)
IHC analysis of a formain fixed paraffin embedded (FFPE) tissue section of mouse brain using ab72182 at 1:1300 dilution. The primary antibody bound to FAM3C protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This FAM3C antibody generated a diffused cytoplasmic staining in the glial cells as well as in the neuronal cells. The signal was more intense in neurons with some level of puncta formation which signifies the presence of FAM3C in the cytoplasmic vesicles
Western blot - Anti-FAM3C/ILEI antibody (ab72182)
All lanes: Western blot - Anti-FAM3C/ILEI antibody (ab72182) at 0.5 µg/mL
Lane 1: Human brain lysate
Lane 2: Human brain lysate with immunising peptide
Lane 3: Mouse brain lysate
Lane 4: Rat brain lysate
Predicted band size: 25 kDa
Observed band size: 30 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)
ab72182 at 5μg/ml staining FAM3C/ILEI in normal human brain by Immunohistochemistry using paraffin-embedded tissue. (Control rabbit Ig used for figure B).
Western blot - Anti-FAM3C/ILEI antibody (ab72182)
All lanes: Western blot - Anti-FAM3C/ILEI antibody (ab72182) at 2 µg/mL
Lane 1: Human testis tissue lysate with 5% non-fat milk in TBST
Lane 2: Mouse testis tissue lysate with 5% non-fat milk in TBST
SecondaryAll lanes: anti-rabbit HRP
Predicted band size: 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (ab72182)
ab72182 at 10μg/ml staining FAM3C/ILEI in human skin by Immunohistochemistry using paraffin-embedded tissue.
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