AB72182

Anti-FAM3C/ILEI antibody

Name: Anti-FAM3C/ILEI antibody (ab72182) | Abcam
Brand: Abcam Limited
SKU: AB72182
Price: 545 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(11 Publications)

Rabbit Polyclonal FAM3C/ILEI antibody. Suitable for WB, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 11 publications.

View Alternative Names

ILEI, GS3786, FAM3C, Protein FAM3C, Interleukin-like EMT inducer

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (AB72182)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (AB72182)

ab72182 at 5μg/ml staining FAM3C/ILEI in normal human brain by Immunohistochemistry using paraffin-embedded tissue. (Control rabbit Ig used for figure B).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (AB72182)

ab72182 at 10μg/ml staining FAM3C/ILEI in human skin by Immunohistochemistry using paraffin-embedded tissue.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (AB72182)

IHC analysis of a formain fixed paraffin embedded (FFPE) tissue section of mouse brain using ab72182 at 1 : 1300 dilution. The primary antibody bound to FAM3C protein in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This FAM3C antibody generated a diffused cytoplasmic staining in the glial cells as well as in the neuronal cells. The signal was more intense in neurons with some level of puncta formation which signifies the presence of FAM3C in the cytoplasmic vesicles

Supplier Data

Western blot - Anti-FAM3C/ILEI antibody (AB72182)

All lanes:

Western blot - Anti-FAM3C/ILEI antibody (ab72182) at 2 µg/mL

Lane 1:

Human testis tissue lysate

Lane 2:

Mouse testis tissue lysate

Secondary

All lanes:

anti-rabbit HRP

Predicted band size: 25 kDa

false

Unknown

Western blot - Anti-FAM3C/ILEI antibody (AB72182)

All lanes:

Western blot - Anti-FAM3C/ILEI antibody (ab72182) at 0.5 µg/mL

Lane 1:

Human brain lysate

Lane 2:

Human brain lysate with immunising peptide

Lane 3:

Mouse brain lysate

Lane 4:

Rat brain lysate

Predicted band size: 25 kDa

Observed band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-10 µg/mL", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Opossum": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in retinal laminar formation. Promotes epithelial to mesenchymal transition.

See full target information FAM3C

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 13:e70412 PubMed39629744

2024

FAM3C Regulates Glioma Cell Proliferation, Invasion, Apoptosis, and Epithelial Mesenchymal Transition via the Notch Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaochao Xia,Zihao Wang,Lvmeng Song,Yinchuan Cheng,Ping Xiong,Shun Li

International journal of molecular sciences 23: PubMed35563313

2022

CD44+ and CD133+ Non-Small Cell Lung Cancer Cells Exhibit DNA Damage Response Pathways and Dormant Polyploid Giant Cancer Cell Enrichment Relating to Their p53 Status.

Applications

Unspecified application

Species

Unspecified reactive species

Margarita Pustovalova,Taisia Blokhina,Lina Alhaddad,Anna Chigasova,Roman Chuprov-Netochin,Alexander Veviorskiy,Gleb Filkov,Andreyan N Osipov,Sergey Leonov

Journal of translational medicine 20:54 PubMed35093095

2022

The ILEI/LIFR complex induces EMT via the Akt and ERK pathways in renal interstitial fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jieqing Zhou,Hong Jiang,Hongkun Jiang,Yan Fan,Jing Zhang,Xiaoxue Ma,Xuewei Yang,Yu Sun,Xing Zhao

Journal of cellular and molecular medicine 23:3464-3475 PubMed30887707

2019

FAM3C-YY1 axis is essential for TGFβ-promoted proliferation and migration of human breast cancer MDA-MB-231 cells via the activation of HSF1.

Applications

Unspecified application

Species

Unspecified reactive species

Weili Yang,Biaoqi Feng,Yuhong Meng,Junpei Wang,Bin Geng,Qinghua Cui,Hongquan Zhang,Yang Yang,Jichun Yang

Oncogene 38:3794-3811 PubMed30692635

2019

TGFβ promotes breast cancer stem cell self-renewal through an ILEI/LIFR signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Alec N Woosley,Annamarie C Dalton,George S Hussey,Breege V Howley,Bidyut K Mohanty,Simon Grelet,Toros Dincman,Sean Bloos,Shaun K Olsen,Philip H Howe

The Journal of biological chemistry 293:11401-11414 PubMed29871931

2018

Interleukin-like EMT inducer (ILEI) promotes melanoma invasiveness and is transcriptionally up-regulated by upstream stimulatory factor-1 (USF-1).

Applications

Unspecified application

Species

Unspecified reactive species

Ken Noguchi,Toros A Dincman,Annamarie C Dalton,Breege V Howley,Buckley J McCall,Bidyut K Mohanty,Philip H Howe

Cell biochemistry and function 36:46-55 PubMed29336056

2018

ILEI is an important intermediate participating in the formation of TGF-β1-induced renal tubular EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Zhao,Gang Luo,Yan Fan,Xiaoxue Ma,Jieqing Zhou,Hong Jiang

Oncotarget 8:106038-106049 PubMed29285313

2017

FAM3C activates HSF1 to suppress hepatic gluconeogenesis and attenuate hyperglycemia of type 1 diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Chen,Junpei Wang,Weili Yang,Ji Chen,Yuhong Meng,Biaoqi Feng,Yujing Chi,Bin Geng,Yong Zhou,Qinghua Cui,Jichun Yang

PloS one 12:e0177830 PubMed28545079

2017

Interleukin-like EMT inducer regulates partial phenotype switching in MITF-low melanoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Ken Noguchi,Annamarie C Dalton,Breege V Howley,Buckley J McCall,Akihiro Yoshida,J Alan Diehl,Philip H Howe

IUBMB life 69:16-21 PubMed27862841

2016

The ubiquitin ligase UBE4A inhibits prostate cancer progression by targeting interleukin-like EMT inducer (ILEI).

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Sun,Xiaopeng Jia,Qiang Gao,Xing Liu,Lianguo Hou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAM3C/ILEI antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (AB72182)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (AB72182)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM3C/ILEI antibody (AB72182)

Western blot - Anti-FAM3C/ILEI antibody (AB72182)

Western blot - Anti-FAM3C/ILEI antibody (AB72182)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (11)

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