Rabbit Polyclonal FAM49B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CYRIB aa 1-150.
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- International journal of biological sciences 16:739-7512020TASP1 Promotes Gallbladder Cancer Cell Proliferation and Metastasis by Up-regulating FAM49B via PI3K/AKT Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYijian Zhang et. al.PubMed 32071545
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CYRIB aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NUQ9
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
Negatively regulates RAC1 signaling and RAC1-driven cytoskeletal remodeling (PubMed:30250061, PubMed:31285585). Regulates chemotaxis, cell migration and epithelial polarization by controlling the polarity, plasticity, duration and extent of protrusions. Limits Rac1 mediated activation of the Scar/WAVE complex, focuses protrusion signals and regulates pseudopod complexity by inhibiting Scar/WAVE-induced actin polymerization (PubMed:30250061). Protects against Salmonella bacterial infection. Attenuates processes such as macropinocytosis, phagocytosis and cell migration and restrict sopE-mediated bacterial entry (PubMed:31285585). Restricts also infection mediated by Mycobacterium tuberculosis and Listeria monocytogenes (By similarity). Involved in the regulation of mitochondrial dynamics and oxidative stress (PubMed:29059164).
Alternative names
CYRI, FAM49B, BM-009, CYRIB, CYFIP-related Rac1 interactor B, L1
Recommended products
Rabbit Polyclonal FAM49B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CYRIB aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CYRIB aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NUQ9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-FAM49B antibody (ab121299)
All lanes: Western blot - Anti-FAM49B antibody (ab121299) at 1/250 dilution
Lane 1: RT4 cell lysate
Lane 2: Human Plasma lysate
Lane 3: U-251 MG cell lysate
Lane 4: Human Liver lysate
Lane 5: Human Tonsil lysate
Developed using the ECL technique.
Predicted band size: 37 kDa
Immunocytochemistry/ Immunofluorescence - Anti-FAM49B antibody (ab121299)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus. Recommended concentration of ab121299 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM49B antibody (ab121299)
ab121299 at 1/100 dilution staining FAM49B in Paraffin-emebedded Human kidney tissue by Immunohistochemistry.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-FAM49B antibody (ab121299)
FAM49B western blot using anti-FAM49B antibody ab121299. Publication image and figure legend from Zhang, Y., Du, P., et al., 2020, Int J Biol Sci, PubMed 32071545.
ab121299 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab121299 please see the product overview.
TASP1 regulates FAM49B via the PI3K/AKT signaling pathway. (A) The expression levels of TASP1, phosphorylated PI3K (Tyr607)/total PI3K and phosphorylated AKT (Ser473)/total AKT were determined in Lv-shNC and Lv-shTASP1 groups by western blot. (B-D) Myc-TASP1-transfected GBC cells was treated with 20 μM LY294002 for 12 h. The proliferation and migration abilities were reduced. TASP1, phosphorylated PI3K (Tyr607)/total PI3K, phosphorylated AKT (Ser473)/total AKT and FAM49B expression levels were examined by western blot. GAPDH was used as the loading control. (E) The proposed mechanistic scheme of TASP1 regulating FAM49B by activating PI3K/AKT signaling pathway in GBC.
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