Anti-FAM49B antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FAM49B antibody (AB121299)

Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus. Recommended concentration of ab121299 1-4 µg/ml. Cells treated with PFA/Triton X-100.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM49B antibody (AB121299)

ab121299 at 1/100 dilution staining FAM49B in Paraffin-emebedded Human kidney tissue by Immunohistochemistry.

• WB

Unknown

Western blot - Anti-FAM49B antibody (AB121299)

All lanes:

Western blot - Anti-FAM49B antibody (ab121299) at 1/250 dilution

Lane 1:

RT4 cell lysate

Lane 2:

Human Plasma lysate

Lane 3:

U-251 MG cell lysate

Lane 4:

Human Liver lysate

Lane 5:

Human Tonsil lysate

Predicted band size: 37 kDa

true

• WB

CiteAb

Western blot - Anti-FAM49B antibody (AB121299)

FAM49B western blot using anti-FAM49B antibody ab121299. Publication image and figure legend from Zhang, Y., Du, P., et al., 2020, Int J Biol Sci, PubMed 32071545.

ab121299 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab121299 please see the product overview.

TASP1 regulates FAM49B via the PI3K/AKT signaling pathway. (A) The expression levels of TASP1, phosphorylated PI3K (Tyr607)/total PI3K and phosphorylated AKT (Ser473)/total AKT were determined in Lv-shNC and Lv-shTASP1 groups by western blot. (B-D) Myc-TASP1-transfected GBC cells was treated with 20 μM LY294002 for 12 h. The proliferation and migration abilities were reduced. TASP1, phosphorylated PI3K (Tyr607)/total PI3K, phosphorylated AKT (Ser473)/total AKT and FAM49B expression levels were examined by western blot. GAPDH was used as the loading control. (E) The proposed mechanistic scheme of TASP1 regulating FAM49B by activating PI3K/AKT signaling pathway in GBC.

false