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AB186410

Anti-FAM50A/XAP5 antibody [EPR12816]

5

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal FAM50A/XAP5 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

DXS9928E, HXC26, XAP5, FAM50A, Protein FAM50A, Protein HXC-26, Protein XAP-5

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-FAM50A/XAP5 antibody [EPR12816] (AB186410)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FAM50A/XAP5 antibody [EPR12816] (AB186410)

Immunocytochemical analysis of Hela cells fixed in 4% paraformaldehyde labling FAM50A/XAP5 with ab186410 at 1/500 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM50A/XAP5 antibody [EPR12816] (AB186410)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM50A/XAP5 antibody [EPR12816] (AB186410)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FAM50A/XAP5 with ab186410 at 1/500 and ready to use HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (AB186410)
  • WB

Supplier Data

Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (AB186410)

All lanes:

Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

Hela cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 40 kDa

false

Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (AB186410)
  • WB

Supplier Data

Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (AB186410)

All lanes:

Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW 264.7 cell lysate at 10 µg

Lane 3:

PC-12 cell lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 40 kDa

false

  • Carrier free

    Anti-FAM50A/XAP5 antibody [EPR12816] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12816

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probably involved in the regulation of pre-mRNA splicing.
See full target information FAM50A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:1302 PubMed33637726

2021

Combinatorial CRISPR screen identifies fitness effects of gene paralogues.

Applications

Unspecified application

Species

Unspecified reactive species

Nicola A Thompson,Marco Ranzani,Louise van der Weyden,Vivek Iyer,Victoria Offord,Alastair Droop,Fiona Behan,Emanuel Gonçalves,Anneliese Speak,Francesco Iorio,James Hewinson,Victoria Harle,Holly Robertson,Elizabeth Anderson,Beiyuan Fu,Fengtang Yang,Guido Zagnoli-Vieira,Phil Chapman,Martin Del Castillo Velasco-Herrera,Mathew J Garnett,Stephen P Jackson,David J Adams
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com