Rabbit Recombinant Monoclonal FAM50A/XAP5 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Images
![Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (AB186410), expandable thumbnail](https://content.abcam.com/products/images/fam50a-xap5-antibody-epr12816-ab186410--western-blot-img104438.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (AB186410)")
![Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (AB186410), expandable thumbnail](https://content.abcam.com/products/images/fam50a-xap5-antibody-epr12816-ab186410--western-blot-img104341.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (AB186410)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM50A/XAP5 antibody [EPR12816] (AB186410), expandable thumbnail](https://content.abcam.com/products/images/fam50a-xap5-antibody-epr12816-ab186410--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95847.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM50A/XAP5 antibody [EPR12816] (AB186410)")
![Immunocytochemistry/ Immunofluorescence - Anti-FAM50A/XAP5 antibody [EPR12816] (AB186410), expandable thumbnail](https://content.abcam.com/products/images/fam50a-xap5-antibody-epr12816-ab186410--immunocytochemistry-immunofluorescence-img63082.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-FAM50A/XAP5 antibody [EPR12816] (AB186410)")
Publications
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- Nature communications 12:13022021Combinatorial CRISPR screen identifies fitness effects of gene paralogues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicola A Thompson,Marco Ranzani,Louise van der Weyden,Vivek Iyer,Victoria Offord,Alastair Droop,Fiona Behan,Emanuel Gonçalves,Anneliese Speak,Francesco Iorio,James Hewinson,Victoria Harle,Holly Robertson,Elizabeth Anderson,Beiyuan Fu,Fengtang Yang,Guido Zagnoli-Vieira,Phil Chapman,Martin Del Castillo Velasco-Herrera,Mathew J Garnett,Stephen P Jackson,David J AdamsPubMed 33637726
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Probably involved in the regulation of pre-mRNA splicing.
Alternative names
DXS9928E, HXC26, XAP5, FAM50A, Protein FAM50A, Protein HXC-26, Protein XAP-5
Recommended products
Rabbit Recombinant Monoclonal FAM50A/XAP5 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12816
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410), expandable thumbnail](https://content.abcam.com/products/images/fam50a-xap5-antibody-epr12816-ab186410--western-blot-img104438.jpg "Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)")
Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
All lanes: Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410) at 1/10000 dilution
Lane 1: Jurkat cell lysate at 20 µg
Lane 2: Hela cell lysate at 20 µg
Lane 3: 293 cell lysate at 20 µg
Lane 4: HepG2 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 40 kDa
![Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410), expandable thumbnail](https://content.abcam.com/products/images/fam50a-xap5-antibody-epr12816-ab186410--western-blot-img104341.jpg "Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)")
Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
All lanes: Western blot - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410) at 1/1000 dilution
Lane 1: C6 cell lysate at 10 µg
Lane 2: RAW 264.7 cell lysate at 10 µg
Lane 3: PC-12 cell lysate at 10 µg
Lane 4: NIH/3T3 cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 40 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410), expandable thumbnail](https://content.abcam.com/products/images/fam50a-xap5-antibody-epr12816-ab186410--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img95847.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling FAM50A/XAP5 with ab186410 at 1/500 and ready to use HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410), expandable thumbnail](https://content.abcam.com/products/images/fam50a-xap5-antibody-epr12816-ab186410--immunocytochemistry-immunofluorescence-img63082.jpg "Immunocytochemistry/ Immunofluorescence - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)")
Immunocytochemistry/ Immunofluorescence - Anti-FAM50A/XAP5 antibody [EPR12816] (ab186410)
Immunocytochemical analysis of Hela cells fixed in 4% paraformaldehyde labling FAM50A/XAP5 with ab186410 at 1/500 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 dilution.
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