JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250057

Anti-FAM50A/XAP5 antibody [EPR12817] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal FAM50A/XAP5 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DXS9928E, HXC26, XAP5, FAM50A, Protein FAM50A, Protein HXC-26, Protein XAP-5

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-FAM50A/XAP5 antibody [EPR12817] - BSA and Azide free (AB250057)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FAM50A/XAP5 antibody [EPR12817] - BSA and Azide free (AB250057)

This data was developed using ab178683, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HepG2 cells labeling FAM50A/XAP5 with ab178683 at 1/100 dilution.

Western blot - Anti-FAM50A/XAP5 antibody [EPR12817] - BSA and Azide free (AB250057)
  • WB

Supplier Data

Western blot - Anti-FAM50A/XAP5 antibody [EPR12817] - BSA and Azide free (AB250057)

This data was developed using ab178683, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FAM50A/XAP5 antibody [EPR12817] (<a href='/en-us/products/primary-antibodies/fam50a-xap5-antibody-epr12817-ab178683'>ab178683</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysates at 10 µg

Lane 2:

293T cell lysates at 10 µg

Lane 3:

Jurkat cell lysates at 10 µg

Lane 4:

HepG2 cell lysates at 10 µg

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12817

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

ab250057 is the carrier-free version of ab178683.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Probably involved in the regulation of pre-mRNA splicing.
See full target information FAM50A
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 3: PubMed25206958

2014

Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures.

Applications

Unspecified application

Species

Unspecified reactive species

Marijke I Zonneveld,Alain R Brisson,Martijn J C van Herwijnen,Sisareuth Tan,Chris H A van de Lest,Frank A Redegeld,Johan Garssen,Marca H M Wauben,Esther N M Nolte-'t Hoen
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB300153

Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free)

RabMAb|Recombinant

Immunoprecipitation - Anti-Ago1 antibody [EPR25441-8] (BSA and Azide free) (AB300153)

  • 0
  • 0
Primary Antibodies

AB4174

Anti-Histone H2A.Z antibody - ChIP Grade

Western blot - Anti-Histone H2A.Z antibody - ChIP Grade (AB4174)

  • 4
  • 31
Primary Antibodies

AB259265

Anti-MDM2 antibody [EPR22256-98]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-MDM2 antibody [EPR22256-98] (AB259265)

  • 5
  • 5
Primary Antibodies

AB191698

Anti-TET1 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TET1 antibody - C-terminal (AB191698)

  • 4
  • 2

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com