Rabbit Polyclonal FAM63B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MINDY2 aa 150-300.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MINDY2 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NBR6
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
Hydrolase that can remove 'Lys-48'-linked conjugated ubiquitin from proteins (PubMed:27292798). Binds to polyubiquitin chains of different linkage types, including 'Lys-6', 'Lys-11', 'Lys-29', 'Lys-33', 'Lys-48' and 'Lys-63' (PubMed:28082312). May play a regulatory role at the level of protein turnover (PubMed:27292798).
Alternative names
FAM63B, KIAA1164, MINDY2, Ubiquitin carboxyl-terminal hydrolase MINDY-2, Deubiquitinating enzyme MINDY-2, Protein FAM63B
Recommended products
Rabbit Polyclonal FAM63B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MINDY2 aa 150-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MINDY2 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NBR6
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM63B antibody (ab151122)
Paraffin embedded human prostate tissue stained for FAM63B using ab151122 at 1/500 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM63B antibody (ab151122)
Paraffin embedded human placenta tissue stained for FAM63B using ab151122 at 1/500 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM63B antibody (ab151122)
Paraffin embedded human testis tissue stained for FAM63B using ab151122 at 1/500 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-FAM63B antibody (ab151122)
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM63B antibody (ab151122)
Paraffin embedded human liver tissue stained for FAM63B using ab151122 at 1/500 dilution in immunohistochemical analysis. Shows no positivity in hepatocytes cells as expected.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before IHC staining protocol.
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