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AB151122

Anti-FAM63B antibody

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(1 Publication)

Rabbit Polyclonal FAM63B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MINDY2 aa 150-300.

View Alternative Names

FAM63B, KIAA1164, MINDY2, Ubiquitin carboxyl-terminal hydrolase MINDY-2, Deubiquitinating enzyme MINDY-2, Protein FAM63B

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM63B antibody (AB151122)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM63B antibody (AB151122)

Paraffin embedded human prostate tissue stained for FAM63B using ab151122 at 1/500 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM63B antibody (AB151122)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM63B antibody (AB151122)

Paraffin embedded human liver tissue stained for FAM63B using ab151122 at 1/500 dilution in immunohistochemical analysis. Shows no positivity in hepatocytes cells as expected.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-FAM63B antibody (AB151122)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FAM63B antibody (AB151122)

Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM63B antibody (AB151122)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM63B antibody (AB151122)

Paraffin embedded human placenta tissue stained for FAM63B using ab151122 at 1/500 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM63B antibody (AB151122)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM63B antibody (AB151122)

Paraffin embedded human testis tissue stained for FAM63B using ab151122 at 1/500 dilution in immunohistochemical analysis.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MINDY2 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q8NBR6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hydrolase that can remove 'Lys-48'-linked conjugated ubiquitin from proteins (PubMed : 27292798). Binds to polyubiquitin chains of different linkage types, including 'Lys-6', 'Lys-11', 'Lys-29', 'Lys-33', 'Lys-48' and 'Lys-63' (PubMed : 28082312). May play a regulatory role at the level of protein turnover (PubMed : 27292798).
See full target information MINDY2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 43:330 PubMed39721999

2024

OTULIN confers cisplatin resistance in osteosarcoma by mediating GPX4 protein homeostasis to evade the mitochondrial apoptotic pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zehang Zheng,Yunhao Zeng,Xing Bao,Chuang Huang,Fengjing Guo,Fei Xu,Zhengqiang Luo
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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