AB236882

Anti-FAM83D antibody

Name: Anti-FAM83D antibody (ab236882) | Abcam
Brand: Abcam Limited
SKU: AB236882
Price: 510 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal FAM83D antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAM83D aa 300-500.

View Alternative Names

C20orf129, FAM83D, Protein FAM83D, Spindle protein CHICA

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-FAM83D antibody (AB236882)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FAM83D antibody (AB236882)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for FAM83D (green) using ab236882 at a dilution of 1/133 in ICC/IF. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

Supplier Data

Western blot - Anti-FAM83D antibody (AB236882)

All lanes:

Western blot - Anti-FAM83D antibody (ab236882) at 1/500 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 64 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FAM83D aa 300-500. The exact immunogen used to generate this antibody is proprietary information.

Q9H4H8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAM83D also known as Family with Sequence Similarity 83 Member D is an important protein involved in cellular processes. It is a protein of significant mass approximately 68 kDa and is highly expressed in the nucleus of proliferating cells. FAM83D's role is associated with cell cycle regulation and it functions effectively in maintaining proper chromosome segregation during mitosis. The protein shows higher expression in rapidly dividing tissues suggesting a link to cell division and growth mechanisms.

Biological function summary

FAM83D participates in vital cellular functions related to mitosis and cancer cell proliferation. It does not function alone but is part of the spindle assembly complex ensuring proper mitotic spindle formation and stability. These interactions highlight its importance in maintaining genomic integrity during cell division reducing the potential for errors that can lead to tumorigenesis. The involvement in this complex makes FAM83D an attractive target for studying cancer biology.

Pathways

FAM83D interacts closely with several key proteins governing cell cycle progression including the Aurora kinase pathway and the Plk1 pathway. It plays a role in phosphorylating and activating proteins necessary for mitotic entry and progression. FAM83D's interaction with Aurora B kinase is particularly critical as it contributes to proper chromosomal alignment and segregation linking it to important regulatory mechanisms in cell proliferation.

Aberrant expression of FAM83D contributes to cancer development including breast cancer and colorectal cancer. Abnormal FAM83D levels disrupt normal cell cycle regulation leading to uncontrolled cell growth. Additionally its interaction with cancer-related proteins such as Cyclin B1 highlights its significance in malignant cells offering potential pathways for therapeutic intervention. Understanding FAM83D's roles and relationships may offer insight into diagnostic and treatment strategies for these cancers.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Through the degradation of FBXW7, may act indirectly on the expression and downstream signaling of MTOR, JUN and MYC (PubMed : 24344117). May play also a role in cell proliferation through activation of the ERK1/ERK2 signaling cascade (PubMed : 25646692). May also be important for proper chromosome congression and alignment during mitosis through its interaction with KIF22 (PubMed : 18485706).

See full target information FAM83D

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 22:101454 PubMed35617811

2022

Suppression of FAM83D Inhibits Glioma Proliferation, Invasion and Migration by Regulating the AKT/mTOR Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Li,Cui Sun,Jing Chen,Ji-Fen Ma,Yi-Heng Pan

Yonsei medical journal 63:282-291 PubMed35184431

2022

MiR-322-5p Alleviates Cell Injury and Impairment of Cognitive Function in Vascular Dementia by Targeting TSPAN5.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zheng,Jie Zhang,Bin Zhou,Huanxian Chang

OncoTargets and therapy 13:4665-4678 PubMed32547096

2020

Knockdown of FAM83D Enhances Radiosensitivity in Coordination with Irradiation by Inhibiting EMT via the Akt/GSK-3β/Snail Signaling Pathway in Human Esophageal Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xing-Xiao Yang,Ming Ma,Mei-Xiang Sang,Xue-Yuan Zhang,Nai-Yi Zou,Shu-Chai Zhu

Journal of experimental & clinical cancer research 39:55 PubMed32228656

2020

circFOXM1 promotes proliferation of non-small cell lung carcinoma cells by acting as a ceRNA to upregulate FAM83D.

Applications

Unspecified application

Species

Unspecified reactive species

Chengtao Yu,Zhuoan Cheng,Shaohua Cui,Xiaowei Mao,Botai Li,Yujie Fu,Hui Wang,Haojie Jin,Qing Ye,Xiaojing Zhao,Liyan Jiang,Wenxin Qin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAM83D antibody

Immunocytochemistry/ Immunofluorescence - Anti-FAM83D antibody (AB236882)

Western blot - Anti-FAM83D antibody (AB236882)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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