AB121750

Anti-FAM83G/PAWS1 antibody

Name: Anti-FAM83G/PAWS1 antibody (ab121750) | Abcam
Brand: Abcam Limited
SKU: AB121750
Rating: 4 (1 reviews)

(1 Review)

(7 Publications)

Rabbit Polyclonal FAM83G/PAWS1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

PAWS1, FAM83G, Protein FAM83G, Protein associated with SMAD1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-FAM83G/PAWS1 antibody (AB121750)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FAM83G/PAWS1 antibody (AB121750)

Immunocytochemistry/Immunofluorescence analysis of U-251 MG cells labelling FAM83G/PAWS1 with ab121750 shows positivity in the cytoplasm.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM83G/PAWS1 antibody (AB121750)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM83G/PAWS1 antibody (AB121750)

ab121750 at 1/100 dilution staining FAM83G/PAWS1 in Paraffin-emebedded Human oral mucosa tissue by Immunohistochemistry.

Unknown

Western blot - Anti-FAM83G/PAWS1 antibody (AB121750)

10-20% SDS PAGE

All lanes:

Western blot - Anti-FAM83G/PAWS1 antibody (ab121750) at 1/250 dilution

Lane 1:

RT4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human Plasma lysate

Lane 4:

Human Liver lysate

Lane 5:

Human Tonsil lysate

Predicted band size: 91 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "Use buffer at pH 6 for antigen retrieval. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein FAM83G also known as PAWS1 is an important regulator in cellular processes. It boasts a mass of approximately 72 kDa and is expressed in various tissue types suggesting a role in broad physiological contexts. FAM83G/PAWS1 acts mechanically by interacting with key cellular machinery which influences various signaling pathways important for maintaining cellular homeostasis.

Biological function summary

The protein interacts with important signaling complexes including the Wnt pathway components. FAM83G/PAWS1 is involved in modulating cellular response to external signals contributing to development and cellular differentiation. It binds to other proteins facilitating the assembly of complexes necessary for effective signal transduction which can impact how cells grow and evolve.

Pathways

FAM83G/PAWS1 is a significant player in the Wnt and TGF-beta signaling cascades. It interacts with proteins such as SMAD2/3 and Gbeta like proteins linking these pathways to broader cellular responses. These interactions ensure the fine-tuning of essential processes like cell positioning during development and signal synchronization in the cellular environment.

FAM83G/PAWS1 shows potential connections to cancer and fibrotic diseases. Its regulation of pathways like Wnt can impact oncogenesis making it a target for cancer research. The protein can also relate to abnormal fibroblast behavior in fibrosis possibly interacting with other proteins such as SMAD3 which is known to play a role in fibrotic response. Understanding FAM83G/PAWS1's role aids in dissecting its contribution to these complex disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Substrate for type I BMP receptor kinase involved in regulation of some target genes of the BMP signaling pathway. Also regulates the expression of several non-BMP target genes, suggesting a role in other signaling pathways.

See full target information FAM83G

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Open biology 14:240075 PubMed39043225

2024

A novel FAM83G variant from palmoplantar keratoderma patient disrupts WNT signalling via loss of FAM83G-CK1α interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Lorraine Glennie,Marta Codina Solà,Mar Xunclà,Gloria Aparicio Español,Elena Garcia-Arumí,Eduardo Fidel Tizzano,Nicola T Wood,Thomas J Macartney,Amaia Lasa-Aranzasti,Gopal P Sapkota

Life science alliance 4: PubMed33361109

2020

FAM83F regulates canonical Wnt signalling through an interaction with CK1α.

Applications

Unspecified application

Species

Unspecified reactive species

Karen Dunbar,Rebecca A Jones,Kevin Dingwell,Thomas J Macartney,James C Smith,Gopal P Sapkota

Life science alliance 4: PubMed33361334

2020

IMiDs induce FAM83F degradation via an interaction with CK1α to attenuate Wnt signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Karen Dunbar,Thomas J Macartney,Gopal P Sapkota

Molecules (Basel, Switzerland) 25: PubMed32570757

2020

FAM83G Is a Novel Inducer of Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Junichi Okada,Noriaki Sunaga,Eijiro Yamada,Tsugumichi Saito,Atsushi Ozawa,Yasuyo Nakajima,Kazuya Okada,Jeffrey E Pessin,Shuichi Okada,Masanobu Yamada

Wellcome open research 4:133 PubMed31656861

2019

Pathogenic palmoplantar keratoderma mutations inhibit the PAWS1:CK1α association and attenuate Wnt signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Z L Wu,Rebecca A Jones,Theresa Tachie-Menson,Thomas J Macartney,Nicola T Wood,Joby Varghese,Robert Gourlay,Renata F Soares,James C Smith,Gopal P Sapkota

EMBO reports 19: PubMed29514862

2018

PAWS1 controls Wnt signalling through association with casein kinase 1α.

Applications

ICC/IF

Species

Unspecified reactive species

Polyxeni Bozatzi,Kevin S Dingwell,Kevin Zl Wu,Fay Cooper,Timothy D Cummins,Luke D Hutchinson,Janis Vogt,Nicola T Wood,Thomas J Macartney,Joby Varghese,Robert Gourlay,David G Campbell,James C Smith,Gopal P Sapkota

The Journal of investigative dermatology 138:984-987 PubMed29138053

2017

Recessive Mutation in FAM83G Associated with Palmoplantar Keratoderma and Exuberant Scalp Hair.

Applications

IHC-P

Species

Human

Thiviyani Maruthappu,Lisa A McGinty,Diana C Blaydon,Benjamin Fell,Arto Määttä,Rebecca Duit,Tim Hawkins,Kristin M Braun,Michael A Simpson,Edel A O'Toole,David P Kelsell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAM83G/PAWS1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-FAM83G/PAWS1 antibody (AB121750)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM83G/PAWS1 antibody (AB121750)

Western blot - Anti-FAM83G/PAWS1 antibody (AB121750)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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