AB121816

Anti-FAM83H antibody

Name: Anti-FAM83H antibody (ab121816) | Abcam
Brand: Abcam Limited
SKU: AB121816

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(2 Publications)

Rabbit Polyclonal FAM83H antibody. Suitable for WB, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAM83H aa 1050 to C-terminus.

View Alternative Names

Protein FAM83H, FAM83H

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM83H antibody (AB121816)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM83H antibody (AB121816)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labeling FAM83H with ab121816 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM83H antibody (AB121816)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue labeling FAM83H with ab121816 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM83H antibody (AB121816)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human duodenum tissue labeling FAM83H with ab121816 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM83H antibody (AB121816)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeeltal muscle tissue labeling FAM83H with ab121816 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-FAM83H antibody (AB121816)

All lanes:

Western blot - Anti-FAM83H antibody (ab121816) at 0.4 µg/mL

All lanes:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Predicted band size: 127 kDa

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Supplier Data

Western blot - Anti-FAM83H antibody (AB121816)

All lanes:

Western blot - Anti-FAM83H antibody (ab121816) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

NBT-II (rat urinary bladder tumor cell line) whole cell lysate

Predicted band size: 127 kDa

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CiteAb

Western blot - Anti-FAM83H antibody (AB121816)

FAM83H western blot using anti-FAM83H antibody ab121816. Publication image and figure legend from Feng, B., Wang, G., et al., 2020, J Cell Mol Med, PubMed 32583631.

ab121816 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab121816 please see the product overview.

FAM83H implicates in ESCC progression and is regulated by FAM83H-AS1 at mRNA and protein level. A, The interfering efficiency against FAM83H analysed by qRT-PCR method. B, Cell proliferation was assessed using MTS assay with silenced FAM83H in Kyse150 and TE1 cells. C, Transwell migration and D, invasion assays were carried out with silenced FAM83H in Kyse150 and TE1 cells (magnification, ×200). E, and F, The influence of FAM83H-AS1 on FAM83H mRNA and protein level detected by qRT-PCR and Western blot assays. G, The influence of FAM83H on FAM83H-AS1 expression analysed by qRT-PCR method. Data are shown as mean ± SD; *p < .05 and **p < .01

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FAM83H aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6ZRV2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAM83H also known as Family with Sequence Similarity 83 Member H is a protein with a mass of approximately 127 kDa. This protein is found mainly in dental tissues including ameloblasts where it plays a role in enamel formation. Its expression can also be detected in other tissues indicating its importance beyond enamel mineralization. FAM83H-localized at the cell membrane involves interactions with other proteins contributing to cellular processes.

Biological function summary

FAM83H acts as an important player in the mineralization of enamel. It may not seem like it but during enamel development FAM83H ensures proper structural integrity and stability of the enamel matrix. FAM83H is not a part of any complex but associates with other proteins such as casein kinase 1 which phosphorylates it regulating its activity in the amelogenesis process. By maintaining normal enamel development FAM83H aids in dental health and disease prevention.

Pathways

FAM83H functions in processes related to enamel formation and dental tissue development. This protein associates with the Wnt signaling pathway important for regulating cell proliferation and differentiation in various tissues. FAM83H interacts with proteins like Axin an important component of the Wnt pathway modulating cell signaling for proper tooth development. Wnt pathway's influence is detectable in many biological processes confirming FAM83H's role in maintaining essential signaling networks within cells.

FAM83H mutations link to amelogenesis imperfecta a disorder characterized by defective enamel formation. This condition can lead to issues such as abnormal tooth appearance and increased susceptibility to dental caries. FAM83H mutations alter gene functionality impacting its interaction with proteins like casein kinase 1. Another disease associated with FAM83H is Ehlers-Danlos syndrome though this connection is less direct but noteworthy involving extracellular matrix proteins. Understanding FAM83H’s involvement in these disorders aids in the development of diagnostic and therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

May play a major role in the structural organization and calcification of developing enamel (PubMed : 18252228). May play a role in keratin cytoskeleton disassembly by recruiting CSNK1A1 to keratin filaments. Thereby, it may regulate epithelial cell migration (PubMed : 23902688).

See full target information FAM83H

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

mBio 13:e0188822 PubMed36154443

2022

Interferon-Inducible E3 Ligase RNF213 Facilitates Host-Protective Linear and K63-Linked Ubiquitylation of Toxoplasma gondii Parasitophorous Vacuoles.

Applications

Unspecified application

Species

Unspecified reactive species

Dulcemaria Hernandez,Stephen Walsh,Luz Saavedra Sanchez,Mary S Dickinson,Jörn Coers

Journal of cellular and molecular medicine : PubMed32583631

2020

LncRNA FAM83H-AS1 promotes oesophageal squamous cell carcinoma progression via miR-10a-5p/Girdin axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Feng,Gaoyan Wang,Xiaoliang Liang,Zheng Wu,Xinchen Wang,Zhiming Dong,Yanli Guo,Supeng Shen,Jia Liang,Wei Guo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAM83H antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM83H antibody (AB121816)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM83H antibody (AB121816)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM83H antibody (AB121816)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM83H antibody (AB121816)

Western blot - Anti-FAM83H antibody (AB121816)

Western blot - Anti-FAM83H antibody (AB121816)

Western blot - Anti-FAM83H antibody (AB121816)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise