AB179833

Anti-FAM98B antibody [EPR12872]

RabMAb
Recombinant
What is this?

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(2 Publications )

Rabbit Recombinant Monoclonal FAM98B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM98B antibody [EPR12872] (AB179833)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM98B antibody [EPR12872] (AB179833)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling FAM98B with ab179833 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-FAM98B antibody [EPR12872] (AB179833)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FAM98B antibody [EPR12872] (AB179833)

Immunofluorescent analysis of Caco-2 cells labeling FAM98B with ab179833 at 1/50 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM98B antibody [EPR12872] (AB179833)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling FAM98B with ab179833 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-FAM98B antibody [EPR12872] (AB179833)

All lanes:

Western blot - Anti-FAM98B antibody [EPR12872] (ab179833) at 1/1000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

Caco-2 cell lysate at 10 µg

Lane 3:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP-conjugated at 1/2000 dilution

Predicted band size: 37 kDa

false

Carrier free

Anti-FAM98B antibody [EPR12872] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12872

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAM98B also known as 'family with sequence similarity 98 member B' is a protein with an approximate mass of 55 kDa. It shows expression in several human tissues with notable levels in the brain liver and lungs. Mechanically FAM98B binds RNA and potentially acts as a scaffold for other protein interactions suggesting roles in RNA processing or regulation.

Biological function summary

FAM98B takes part in various cellular processes including the assembly of RNA-protein complexes. It associates with the CCR4-NOT complex a critical regulator of mRNA deadenylation and decay. Through these interactions FAM98B possibly influences mRNA stability and gene expression impacting cell growth and differentiation.

Pathways

Researchers have linked FAM98B to pathways like mRNA surveillance and cell cycle regulation. It interacts with proteins such as CNOT1 and other components of the CCR4-NOT complex. This association plays a role in monitoring mRNA integrity and the promotion of mRNA decay affecting cell proliferation and response to stress.

Alterations in FAM98B expression or function relate to cancer and neurodegenerative disease. In cancer abnormal levels of FAM98B might influence tumor growth by interacting with proteins like DDX20 affecting cell cycle regulation. In neurodegenerative disorders disruptions in pathways involving FAM98B could impact neuronal function possibly interacting with proteins within the CCR4-NOT complex to influence cognitive outcomes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accessory subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity and may function toward other RNAs (PubMed : 21311021, PubMed : 24870230). Could regulate the expression of PRMT1, a protein arginine N-methyltransferase (PubMed : 28040436).

See full target information FAM98B

Alternative Names

tRNA-splicing ligase complex subunit FAM98B, FAM98B

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Publications (2)

Recent publications for all applications. Explore the full listand refine your search

Breast cancer research and treatment 184:237-248 PubMed32757134

2020

Effect of functional variant rs11466313 on breast cancer susceptibility and TGFB1 promoter activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ting Zhou,Li-Yuan Zheng,Ya-Jun Wang,Li Yang,Yun-Tao Xie,Ipsita Panda,Xin-Xia Tian,Wei-Gang Fang

Frontiers in physiology 10:92 PubMed30833903

2019

hCLE/RTRAF-HSPC117-DDX1-FAM98B: A New Cap-Binding Complex That Activates mRNA Translation.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandra Pazo,Alicia Pérez-González,Juan Carlos Oliveros,Maite Huarte,Juan Pablo Chavez,Amelia Nieto

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAM98B antibody [EPR12872]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM98B antibody [EPR12872] (AB179833)

Immunocytochemistry/ Immunofluorescence - Anti-FAM98B antibody [EPR12872] (AB179833)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAM98B antibody [EPR12872] (AB179833)

Western blot - Anti-FAM98B antibody [EPR12872] (AB179833)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (2)

Product promise