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AB68572

Anti-FAN1 antibody

4

(1 Review)

|

(2 Publications)

Mouse Polyclonal FAN1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FAN1.

View Alternative Names

KIAA1018, MTMR15, FAN1, Fanconi-associated nuclease 1, FANCD2/FANCI-associated nuclease 1, Myotubularin-related protein 15, hFAN1

2 Images
Western blot - Anti-FAN1 antibody (AB68572)
  • WB

Unknown

Western blot - Anti-FAN1 antibody (AB68572)

All lanes:

Western blot - Anti-FAN1 antibody (ab68572) at 1/500 dilution

All lanes:

Human spleen lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 114 kDa

Observed band size: 114 kDa

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Western blot - Anti-FAN1 antibody (AB68572)
  • WB

Unknown

Western blot - Anti-FAN1 antibody (AB68572)

All lanes:

Western blot - Anti-FAN1 antibody (ab68572) at 1/500 dilution

Lane 1:

MTMR15 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 114 kDa

Observed band size: 70 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FAN1.

Q9Y2M0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FAN1 also known as Fanconi-associated nuclease 1 is a protein implicated in DNA repair processes. The protein has a molecular mass of approximately 101 kDa. FAN1 can be found in various tissues with enriched expression in testis thymus and liver. It operates as a nuclease engaging in processes to remove DNA crosslinks maintaining the integrity of genetic material.
Biological function summary

FAN1 plays a significant role in the repair of interstrand cross-links within DNA. FAN1 acts as part of a complex that detects and processes these cross-links aiding in cell survival by preventing genome instability. It connects with the Fanconi anemia pathway proteins which include FANCD2 and FANCI to promote the repair process. The function of FAN1 is critical in preserving cellular health under conditions that could potentially damage DNA.

Pathways

FAN1 exists prominently in the DNA damage response and the Fanconi anemia pathway. It interacts closely with proteins such as MLH1 tying into a broader network of repair mechanisms. These pathways are essential for cellular resistance to various stressors that threaten genomic stability. FAN1's precise involvement ensures cells can effectively manage DNA damage contributing to proper functioning of genetic repair networks.

FAN1 displays connections with two significant conditions: Fanconi anemia and colorectal cancer. Mutations or deficiencies in FAN1 can lead to increased susceptibility to genomic instability disorders like Fanconi anemia. The protein also associates with proteins like PMS2 and MLH1 which when mutated contribute to colorectal cancer. Understanding FAN1's role offers insight into therapeutic targets and genetic predispositions related to these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates (PubMed : 20603015, PubMed : 20603016, PubMed : 20603073, PubMed : 20671156, PubMed : 24981866, PubMed : 25430771). Not involved in DNA double-strand breaks resection (PubMed : 20603015, PubMed : 20603016). Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL (PubMed : 25430771). Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap (PubMed : 25430771). Also has endonuclease activity toward 5'-flaps (PubMed : 20603015, PubMed : 20603016, PubMed : 24981866).
See full target information FAN1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 80:327-344.e8 PubMed32966758

2020

PCAF-Mediated Histone Acetylation Promotes Replication Fork Degradation by MRE11 and EXO1 in BRCA-Deficient Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jae Jin Kim,Seo Yun Lee,Ji-Hye Choi,Hyun Goo Woo,Blerta Xhemalce,Kyle M Miller

Archives of medical science : AMS 15:1035-1046 PubMed31360198

2019

Microarray gene profiling analysis of glioblastoma cell line U87 reveals suppression of the /Fanconi anemia pathway by the combination of Y15 and temozolomide.

Applications

Unspecified application

Species

Unspecified reactive species

Zichuan Wang,Nan Chen,Jin Yang,Qingzhong Wang,Aimin Li
View all publications
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