Mouse Polyclonal FAN1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FAN1.
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Publications
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- Molecular cell 80:327-344.e82020PCAF-Mediated Histone Acetylation Promotes Replication Fork Degradation by MRE11 and EXO1 in BRCA-Deficient Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJae Jin Kim,Seo Yun Lee,Ji-Hye Choi,Hyun Goo Woo,Blerta Xhemalce,Kyle M MillerPubMed 32966758
- Archives of medical science : AMS 15:1035-10462019Microarray gene profiling analysis of glioblastoma cell line U87 reveals suppression of the /Fanconi anemia pathway by the combination of Y15 and temozolomide.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZichuan Wang et. al.PubMed 31360198
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human FAN1. Database link Q9Y2M0
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. Specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates (PubMed:20603015, PubMed:20603016, PubMed:20603073, PubMed:20671156, PubMed:24981866, PubMed:25430771). Not involved in DNA double-strand breaks resection (PubMed:20603015, PubMed:20603016). Acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. Probably keeps excising with 3'-flap annealing until it reaches and unhooks the ICL (PubMed:25430771). Acts at sites that have a 5'-terminal phosphate anchor at a nick or a 1- or 2-nucleotide flap and is augmented by a 3' flap (PubMed:25430771). Also has endonuclease activity toward 5'-flaps (PubMed:20603015, PubMed:20603016, PubMed:24981866).
Alternative names
KIAA1018, MTMR15, FAN1, Fanconi-associated nuclease 1, FANCD2/FANCI-associated nuclease 1, Myotubularin-related protein 15, hFAN1
Recommended products
Mouse Polyclonal FAN1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FAN1.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human FAN1. Database link Q9Y2M0
- Carrier free
- Yes
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FAN1 also known as Fanconi-associated nuclease 1 is a protein implicated in DNA repair processes. The protein has a molecular mass of approximately 101 kDa. FAN1 can be found in various tissues with enriched expression in testis thymus and liver. It operates as a nuclease engaging in processes to remove DNA crosslinks maintaining the integrity of genetic material.
- Biological function summary
FAN1 plays a significant role in the repair of interstrand cross-links within DNA. FAN1 acts as part of a complex that detects and processes these cross-links aiding in cell survival by preventing genome instability. It connects with the Fanconi anemia pathway proteins which include FANCD2 and FANCI to promote the repair process. The function of FAN1 is critical in preserving cellular health under conditions that could potentially damage DNA.
- Pathways
FAN1 exists prominently in the DNA damage response and the Fanconi anemia pathway. It interacts closely with proteins such as MLH1 tying into a broader network of repair mechanisms. These pathways are essential for cellular resistance to various stressors that threaten genomic stability. FAN1's precise involvement ensures cells can effectively manage DNA damage contributing to proper functioning of genetic repair networks.
- Associated diseases and disorders
FAN1 displays connections with two significant conditions: Fanconi anemia and colorectal cancer. Mutations or deficiencies in FAN1 can lead to increased susceptibility to genomic instability disorders like Fanconi anemia. The protein also associates with proteins like PMS2 and MLH1 which when mutated contribute to colorectal cancer. Understanding FAN1's role offers insight into therapeutic targets and genetic predispositions related to these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-FAN1 antibody (ab68572)
All lanes: Western blot - Anti-FAN1 antibody (ab68572) at 1/500 dilution
All lanes: Human spleen lysate at 25 µg
SecondaryAll lanes: Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 114 kDa
Observed band size: 114 kDa
Western blot - Anti-FAN1 antibody (ab68572)
All lanes: Western blot - Anti-FAN1 antibody (ab68572) at 1/500 dilution
Lane 1: MTMR15 transfected 293T cell lysate at 25 µg
Lane 2: Non-transfected 293T cell lysate at 25 µg
SecondaryAll lanes: Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 114 kDa
Observed band size: 70 kDa
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