AB264257

Anti-FANCA/FAA antibody

Name: Anti-FANCA/FAA antibody (ab264257) | Abcam
Brand: Abcam Limited
SKU: AB264257

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(1 Publication)

Rabbit Polyclonal FANCA/FAA antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FANCA aa 1400 to C-terminus.

View Alternative Names

FAA, FACA, FANCH, FANCA, Fanconi anemia group A protein, Protein FACA

2 Images

Supplier Data

Immunoprecipitation - Anti-FANCA/FAA antibody (AB264257)

FANCA/FAA was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264257 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab264257 at 0.04 μg/ml.
Lane 1 : ab264257 IP in HeLa whole cell lysate.
Lane 2 : ab264257 IP in HeLa whole cell lysate.
Lane 3 : Control IgG.

Exposure time : 75 secs.

Different lots were used in lane 1 and lane 2.

Cells prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-FANCA/FAA antibody (ab264257)

Predicted band size: 163 kDa

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Supplier Data

Western blot - Anti-FANCA/FAA antibody (AB264257)

Detected with chemilimuniscence with exposure time of 30 seconds.

Cells prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-FANCA/FAA antibody (ab264257) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

K-562 whole cell lysate at 50 µg

Lane 3:

A-549 whole cell lysate at 50 µg

Lane 4:

MCF-7 whole cell lysate at 50 µg

Lane 5:

HEK-293T whole cell lysate at 50 µg

Predicted band size: 163 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human FANCA aa 1400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O15360

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FANCA protein also known as FAA protein is a critical component in the DNA repair mechanism. FANCA is approximately 162 kDa and shows widespread expression in the human body particularly in proliferating cells. This protein plays an essential role in maintaining genomic stability by participating in the repair of DNA interstrand crosslinks. FANCA's primary function involves its interaction with other proteins in the cell nucleus where DNA repair and replication processes occur.

Biological function summary

FANCA works as a part of the Fanconi anemia (FA) core complex involving around 13 proteins. This complex plays an important role in the FA DNA repair pathway facilitating the repair of interstrand DNA crosslinks. FANCA acts by orchestrating the recruitment and activation of downstream repair proteins which ensures the high-fidelity maintenance of the genetic material during cell division. Dysfunction in the FA complex where FANCA operates can lead to chromosomal instability and increased mutation rates.

Pathways

FANCA functions prominently in the Fanconi anemia pathway and is also involved in the homologous recombination repair pathway. In the FA pathway FANCA works closely with related proteins like FANCB and FANCC to initiate the DNA repair process. FANCA's interaction with these proteins allows it to execute its repair role effectively ensuring cellular genomic integrity. These pathways are critical for protecting cells from DNA damage during replication and shielding them from cancer development.

FANCA mutations are directly associated with Fanconi anemia a genetic disorder characterized by marrow failure cancer predisposition and various congenital abnormalities. Loss of FANCA function disrupts the repair pathway leading to cellular hypersensitivity to DNA-damaging agents. Additionally FANCA's impairment has been implicated in certain cancers like acute myeloid leukemia where its interaction with other FA proteins gets compromised leading to tumor progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.

See full target information FANCA

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:286 PubMed40544182

2025

Development of translational read-through-inducing drugs as novel therapeutic options for patients with Fanconi anemia.

Applications

Unspecified application

Species

Unspecified reactive species

Anca Manuela Hristodor,Enrico Cappelli,Elena Baldisseri,Roberto Valli,Giuseppe Montalbano,Giovanni Micheloni,Giovanni Porta,Annalisa Frattini,Silvia Ravera,Francesca Fioredda,Giuseppe Lippi,Carlo Dufour,Marco Cipolli,Valentino Bezzerri

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