AB5063

Anti-FANCA/FAA antibody

Name: Anti-FANCA/FAA antibody (ab5063) | Abcam
Brand: Abcam Limited
SKU: AB5063
Price: 435 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(11 Publications)

Rabbit Polyclonal FANCA/FAA antibody. Suitable for WB, ELISA and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human FANCA aa 950 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

FAA, FACA, FANCH, FANCA, Fanconi anemia group A protein, Protein FACA

1 Images

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Western blot - Anti-FANCA/FAA antibody (AB5063)

All lanes:

Western blot - Anti-FANCA/FAA antibody (ab5063) at 1/1500 dilution

All lanes:

HeLa whole cell lysate at 35 µg

Secondary

All lanes:

IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

Predicted band size: 163 kDa

Observed band size: 130 kDa,42 kDa,75 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ELISA, WB

applications

Immunogen

Synthetic Peptide within Human FANCA aa 950 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O15360

Specificity

Detects a band at 130kDa in HeLa cell lysate that we believe corresponds to FANCA/FAA.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/15000 - 1/60000", "ELISA-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This affinity-purified antibody is directed against human FANCA protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FANCA protein also known as FAA protein is a critical component in the DNA repair mechanism. FANCA is approximately 162 kDa and shows widespread expression in the human body particularly in proliferating cells. This protein plays an essential role in maintaining genomic stability by participating in the repair of DNA interstrand crosslinks. FANCA's primary function involves its interaction with other proteins in the cell nucleus where DNA repair and replication processes occur.

Biological function summary

FANCA works as a part of the Fanconi anemia (FA) core complex involving around 13 proteins. This complex plays an important role in the FA DNA repair pathway facilitating the repair of interstrand DNA crosslinks. FANCA acts by orchestrating the recruitment and activation of downstream repair proteins which ensures the high-fidelity maintenance of the genetic material during cell division. Dysfunction in the FA complex where FANCA operates can lead to chromosomal instability and increased mutation rates.

Pathways

FANCA functions prominently in the Fanconi anemia pathway and is also involved in the homologous recombination repair pathway. In the FA pathway FANCA works closely with related proteins like FANCB and FANCC to initiate the DNA repair process. FANCA's interaction with these proteins allows it to execute its repair role effectively ensuring cellular genomic integrity. These pathways are critical for protecting cells from DNA damage during replication and shielding them from cancer development.

FANCA mutations are directly associated with Fanconi anemia a genetic disorder characterized by marrow failure cancer predisposition and various congenital abnormalities. Loss of FANCA function disrupts the repair pathway leading to cellular hypersensitivity to DNA-damaging agents. Additionally FANCA's impairment has been implicated in certain cancers like acute myeloid leukemia where its interaction with other FA proteins gets compromised leading to tumor progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.

See full target information FANCA

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35955545

2022

Correction of Fanconi Anemia Mutations Using Digital Genome Engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher J Sipe,Mitchell G Kluesner,Samuel P Bingea,Walker S Lahr,Aneesha A Andrew,Minjing Wang,Anthony P DeFeo,Timothy L Hinkel,Kanut Laoharawee,John E Wagner,Margaret L MacMillan,Gregory M Vercellotti,Jakub Tolar,Mark J Osborn,R Scott McIvor,Beau R Webber,Branden S Moriarity

Cell stem cell 25:607-621.e7 PubMed31543367

2019

NHEJ-Mediated Repair of CRISPR-Cas9-Induced DNA Breaks Efficiently Corrects Mutations in HSPCs from Patients with Fanconi Anemia.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco José Román-Rodríguez,Laura Ugalde,Lara Álvarez,Begoña Díez,María José Ramírez,Cristina Risueño,Marta Cortón,Massimo Bogliolo,Sara Bernal,Francesca March,Carmen Ayuso,Helmut Hanenberg,Julián Sevilla,Sandra Rodríguez-Perales,Raúl Torres-Ruiz,Jordi Surrallés,Juan Antonio Bueren,Paula Río

Experimental hematology 43:1031-1046.e12 PubMed26366677

2015

FANCA safeguards interphase and mitosis during hematopoiesis in vivo.

Applications

Species

Unspecified reactive species

Zahi Abdul-Sater,Donna Cerabona,Elizabeth Sierra Potchanant,Zejin Sun,Rikki Enzor,Ying He,Kent Robertson,W Scott Goebel,Grzegorz Nalepa

Stem cell research 13:240-50 PubMed25108529

2014

FANCA knockout in human embryonic stem cells causes a severe growth disadvantage.

Applications

Species

Unspecified reactive species

Kim Vanuytsel,Qing Cai,Nisha Nair,Satish Khurana,Swati Shetty,Joris R Vermeesch,Laura Ordovas,Catherine M Verfaillie

EMBO molecular medicine 6:835-48 PubMed24859981

2014

Targeted gene therapy and cell reprogramming in Fanconi anemia.

Applications

Species

Human

Paula Rio,Rocio Baños,Angelo Lombardo,Oscar Quintana-Bustamante,Lara Alvarez,Zita Garate,Pietro Genovese,Elena Almarza,Antonio Valeri,Begoña Díez,Susana Navarro,Yaima Torres,Juan P Trujillo,Rodolfo Murillas,Jose C Segovia,Enrique Samper,Jordi Surralles,Philip D Gregory,Michael C Holmes,Luigi Naldini,Juan A Bueren

Cancer research 73:3393-401 PubMed23633493

2013

Regulation of FANCD2 by the mTOR pathway contributes to the resistance of cancer cells to DNA double-strand breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Changxian Shen,Duane Oswald,Doris Phelps,Hakan Cam,Christopher E Pelloski,Qishen Pang,Peter J Houghton

Cell cycle (Georgetown, Tex.) 10:3300-10 PubMed21926477

2011

The Fanconi anemia pathway is downregulated upon macrophage differentiation through two distinct mechanisms.

Applications

Species

Human

Wei-Ting Lu,Kimon Lemonidis,Ross M Drayton,Thierry Nouspikel

PloS one 6:e22911 PubMed21826217

2011

Fanconi anemia core complex gene promoters harbor conserved transcription regulatory elements.

Applications

Species

Unspecified reactive species

Daniel Meier,Detlev Schindler

PLoS genetics 6:e1000983 PubMed20548951

2010

The E1A-associated p400 protein modulates cell fate decisions by the regulation of ROS homeostasis.

Applications

Species

Human

Lise Mattera,Céline Courilleau,Gaëlle Legube,Takeshi Ueda,Rikiro Fukunaga,Martine Chevillard-Briet,Yvan Canitrot,Fabrice Escaffit,Didier Trouche

Blood 115:3453-62 PubMed20089964

2010

Knockdown of Fanconi anemia genes in human embryonic stem cells reveals early developmental defects in the hematopoietic lineage.

Applications

Species

Human

Asmin Tulpule,M William Lensch,Justine D Miller,Karyn Austin,Alan D'Andrea,Thorsten M Schlaeger,Akiko Shimamura,George Q Daley

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FANCA/FAA antibody

Western blot - Anti-FANCA/FAA antibody (AB5063)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise