Anti-FANCA/FAA antibody [EPR16519-42] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-FANCA/FAA antibody [EPR16519-42] - BSA and Azide free (AB251345)

This data was developed using ab201458, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FANCA/FAA antibody [EPR16519-42] (<a href='/en-us/products/primary-antibodies/fanca-faa-antibody-epr16519-42-ab201458'>ab201458</a>) at 1/10000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

293 (Human embryonic kidney) whole cell lysate at 20 µg

Lane 3:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Lane 4:

A431 (Human epidermoid carcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 163 kDa

Observed band size: 163 kDa

false

Exposure time: 1min

• WB

Supplier Data

Western blot - Anti-FANCA/FAA antibody [EPR16519-42] - BSA and Azide free (AB251345)

This data was developed using ab201458, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-FANCA/FAA antibody [EPR16519-42] (<a href='/en-us/products/primary-antibodies/fanca-faa-antibody-epr16519-42-ab201458'>ab201458</a>) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 163 kDa

Observed band size: 163 kDa

false

Exposure time: 3min

• WB

Lab

Western blot - Anti-FANCA/FAA antibody [EPR16519-42] - BSA and Azide free (AB251345)

This data was developed using ab201458, the same antibody clone in a different buffer formulation.

Lane 1 Wild-type HAP1 cell lysate (20 μg)

Lane 2 ProteinX knockout HAP1 cell lysate (20 μg)

Lane 3 HeLa cell lysate (20 μg)

Lane 4 Jurkat cell lysate (20 μg)

Lanes 1 - 4 Merged signal (red and green). Green - ab201458 observed at 163 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab201458 was shown to recognize FANCA/FAA when FANCA/FAA knockout samples were used, along with additional cross-reactive bands. Wild-type and FANCA/FAA knockout samples were subjected to SDS-PAGE. ab201458 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-FANCA/FAA antibody [EPR16519-42] (<a href='/en-us/products/primary-antibodies/fanca-faa-antibody-epr16519-42-ab201458'>ab201458</a>)

Predicted band size: 163 kDa

false