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Rabbit Recombinant Monoclonal FANCA/FAA antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

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Images

Western blot - Anti-FANCA/FAA antibody [EPR16519] (AB201457), expandable thumbnail
  • Western blot - Anti-FANCA/FAA antibody [EPR16519] (AB201457), expandable thumbnail
  • Immunoprecipitation - Anti-FANCA/FAA antibody [EPR16519] (AB201457), expandable thumbnail
  • Western blot - Anti-FANCA/FAA antibody [EPR16519] (AB201457), expandable thumbnail
  • Western blot - Anti-FANCA/FAA antibody [EPR16519] (AB201457), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability.

Alternative names

FAA, FACA, FANCH, FANCA, Fanconi anemia group A protein, Protein FACA

Recommended products

Rabbit Recombinant Monoclonal FANCA/FAA antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16519
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The FANCA protein also known as FAA protein is a critical component in the DNA repair mechanism. FANCA is approximately 162 kDa and shows widespread expression in the human body particularly in proliferating cells. This protein plays an essential role in maintaining genomic stability by participating in the repair of DNA interstrand crosslinks. FANCA's primary function involves its interaction with other proteins in the cell nucleus where DNA repair and replication processes occur.

Biological function summary

FANCA works as a part of the Fanconi anemia (FA) core complex involving around 13 proteins. This complex plays an important role in the FA DNA repair pathway facilitating the repair of interstrand DNA crosslinks. FANCA acts by orchestrating the recruitment and activation of downstream repair proteins which ensures the high-fidelity maintenance of the genetic material during cell division. Dysfunction in the FA complex where FANCA operates can lead to chromosomal instability and increased mutation rates.

Pathways

FANCA functions prominently in the Fanconi anemia pathway and is also involved in the homologous recombination repair pathway. In the FA pathway FANCA works closely with related proteins like FANCB and FANCC to initiate the DNA repair process. FANCA's interaction with these proteins allows it to execute its repair role effectively ensuring cellular genomic integrity. These pathways are critical for protecting cells from DNA damage during replication and shielding them from cancer development.

Associated diseases and disorders

FANCA mutations are directly associated with Fanconi anemia a genetic disorder characterized by marrow failure cancer predisposition and various congenital abnormalities. Loss of FANCA function disrupts the repair pathway leading to cellular hypersensitivity to DNA-damaging agents. Additionally FANCA's impairment has been implicated in certain cancers like acute myeloid leukemia where its interaction with other FA proteins gets compromised leading to tumor progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-FANCA/FAA antibody [EPR16519] (ab201457), expandable thumbnail

    Western blot - Anti-FANCA/FAA antibody [EPR16519] (ab201457)

    Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 μg)
    Lanes 2, 6 and 10: FANCA/FAA beta knockout HAP1 cell lysate (20 μg)
    Lanes 3, 7 and 11: HeLa cell lysate (20 μg)
    Lanes 4, 8 and 12: HEK293 cell lysate (20 μg)
    Lanes 1, 2, 3 and 4: Green signal from target – ab201457 observed at 163 kDa
    Lanes 5, 6, 7 and 8: Red signal from loading control – Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa
    Lanes 9, 10, 11 and 12: Merged (red and green) signal

    ab201457 was shown to specifically react with FANCA/FAA beta when FANCA/FAA beta knockout samples were used. Wild-type and FANCA/FAA beta knockout samples were subjected to SDS-PAGE. ab201457 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/10000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-FANCA/FAA antibody [EPR16519] (ab201457)

    Predicted band size: 163 kDa

  • Western blot - Anti-FANCA/FAA antibody [EPR16519] (ab201457), expandable thumbnail

    Western blot - Anti-FANCA/FAA antibody [EPR16519] (ab201457)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-FANCA/FAA antibody [EPR16519] (ab201457) at 1/10000 dilution

    Lane 1: HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

    Lane 3: Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

    Lane 4: A431 (Human epidermoid carcinoma) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 163 kDa

    Observed band size: 163 kDa

    Exposure time: 1min

  • Immunoprecipitation - Anti-FANCA/FAA antibody [EPR16519] (ab201457), expandable thumbnail

    Immunoprecipitation - Anti-FANCA/FAA antibody [EPR16519] (ab201457)

    FANCA/FAA was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab201457 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab201457 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/1500 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (Input). Lane 2: ab201457 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab201457 in HeLa whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-FANCA/FAA antibody [EPR16519] (ab201457)

    Predicted band size: 163 kDa

  • Western blot - Anti-FANCA/FAA antibody [EPR16519] (ab201457), expandable thumbnail

    Western blot - Anti-FANCA/FAA antibody [EPR16519] (ab201457)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-FANCA/FAA antibody [EPR16519] (ab201457) at 1/1000 dilution

    All lanes: Human colon lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 163 kDa

    Observed band size: 163 kDa

    Exposure time: 3min

  • Western blot - Anti-FANCA/FAA antibody [EPR16519] (ab201457), expandable thumbnail

    Western blot - Anti-FANCA/FAA antibody [EPR16519] (ab201457)

    Western blot: Anti-FANCA antibody [EPR16519] (ab201457) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab201457 was shown to bind specifically to FANCA. A band was observed at 163 kDa in wild-type A549 cell lysates with no signal observed at this size in FANCA knockout cell line. To generate this image, wild-type and FANCA knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    Lanes 1 - 4: Western blot - Anti-FANCA/FAA antibody [EPR16519] (ab201457) at 1/1000 dilution

    Lanes 1 - 4: Western blot - Anti-FANCA/FAA antibody [EPR16519] - BSA and Azide free (Anti-FANCA/FAA antibody [EPR16519] - BSA and Azide free ab251344)

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: FANCA knockout A549 cell lysate at 20 µg

    Lane 3: Wild-type HAP1 cell lysate at 20 µg

    Lane 4: FANCA knockout HAP1 cell lysate at 20 µg

    Performed under reducing conditions.

    Observed band size: 163 kDa

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Product protocols

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