JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB151516

Anti-FANCG antibody

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Polyclonal FANCG antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FANCG aa 1-300.

View Alternative Names

XRCC9, FANCG, Fanconi anemia group G protein, Protein FACG, DNA repair protein XRCC9

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-FANCG antibody (AB151516)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FANCG antibody (AB151516)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling FANCG with ab151516 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Western blot - Anti-FANCG antibody (AB151516)
  • WB

Unknown

Western blot - Anti-FANCG antibody (AB151516)

7.5% SDS PAGE

All lanes:

Western blot - Anti-FANCG antibody (ab151516) at 1/500 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 69 kDa

false

Western blot - Anti-FANCG antibody (AB151516)
  • WB

Unknown

Western blot - Anti-FANCG antibody (AB151516)

7.5% SDS PAGE

All lanes:

Western blot - Anti-FANCG antibody (ab151516) at 1/1000 dilution

All lanes:

NIH 3T3 whole cell lysate at 30 µg

Predicted band size: 69 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human FANCG aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

O15287

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FANCG also known as Protein FACG or Fanconi anemia group G protein is a component involved in DNA repair. It has a mass of approximately 68 kDa. This protein is mainly expressed in tissues with high proliferative rates such as bone marrow and germ cells. Mechanically FANCG plays an important role in the maintenance of genomic stability by participating in the repair processes that fix DNA interstrand crosslinks.
Biological function summary

Fanconi anemia group G protein is an essential part of the Fanconi anemia (FA) core complex which consists of several FA proteins. This complex is indispensable for cellular resistance to DNA damage as it repairs broken DNA strands and stalls replication forks. FANCG as part of this multiprotein complex ensures proper cellular responses to genotoxic stresses and helps in preventing mutations that could be passed to daughter cells.

Pathways

FANCG primarily functions within the FA/BRCA pathway an essential mechanism for DNA damage response and repair. It interacts closely with other FA proteins such as FANCA and FANCJ to facilitate the monoubiquitination of the FANCD2-FANCI complex a critical step in the DNA damage repair. Additionally the FA pathway is linked to homologous recombination repair where FANCG indirectly associates with BRCA1 and BRCA2 proteins coordinating with them to maintain genomic integrity.

Defects in FANCG are directly associated with Fanconi anemia a rare genetic disorder characterized by bone marrow failure and increased cancer susceptibility. FANCG mutations disrupt its interaction within the FA complex leading to increased cellular susceptibility to DNA damage. Moreover alterations in FA pathway components including FANCG also relate to specific cancer types such as acute myeloid leukemia. The protein engages in interactions with other FA pathway proteins like FANCD2 affecting disease progress and therapeutic outcomes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Candidate tumor suppressor gene.
See full target information FANCG
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB5067

Anti-FANCG antibody

Western blot - Anti-FANCG antibody (AB5067)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB254349

Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker

BOND RX™ Validated|Lab Essentials|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB254349)

  • 4
  • 1
Primary Antibodies

AB18258

Anti-PSD95 antibody - Synaptic Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PSD95 antibody - Synaptic Marker (AB18258)

  • 5
  • 40
Primary Antibodies

AB32127

Anti-Synaptophysin antibody [YE269] - Synaptic Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB32127)

  • 5
  • 37

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com