AB74332

Anti-FANCI antibody

Name: Anti-FANCI antibody (ab74332) | Abcam
Brand: Abcam Limited
SKU: AB74332
Price: 545 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal FANCI antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human FANCI.

View Alternative Names

KIAA1794, FANCI, Fanconi anemia group I protein, Protein FACI

2 Images

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Immunoprecipitation - Anti-FANCI antibody (AB74332)

Detection of Human KIAA1794 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab74332 at 3 µg/mg for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents IgG control IP.

All lanes:

Immunoprecipitation - Anti-FANCI antibody (ab74332)

Predicted band size: 149 kDa

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Western blot - Anti-FANCI antibody (AB74332)

All lanes:

Western blot - Anti-FANCI antibody (ab74332) at 0.04 µg/mL

Lane 1:

Whole HeLa cell lysate at 50 µg

Lane 2:

Whole HeLa cell lysate at 15 µg

Lane 3:

Whole HeLa cell lysate at 5 µg

Lane 4:

Whole 293T cell lysate at 50 µg

Predicted band size: 149 kDa

Observed band size: 100 kDa,65 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human FANCI. The exact immunogen used to generate this antibody is proprietary information.

Q9NVI1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FANCI also known as Fanconi anemia group I protein is an important player in DNA repair processes. This protein has a molecular mass of approximately 150 kDa. It is ubiquitously expressed with notable levels found in tissues that undergo high rates of cell division. FANCI participates mechanically within the DNA repair mechanism by interacting directly with DNA alongside its partner protein FANCD2 to form a tight complex that recognizes and binds to sites of DNA damage.

Biological function summary

The FANCI protein acts as part of the multiprotein Fanconi anemia (FA) core complex. This complex Functions in the repair of DNA interstrand crosslinks which are severe forms of DNA damage. Successful function of FANCI is important for maintaining genomic stability and subsequently proper cell cycle progression. It partners with proteins including FANCD2 to coordinate the homologous recombination repair process. Defective FANCI leads to disrupted DNA repair resulting in cellular damage and apoptosis.

Pathways

FANCI plays a major role in the Fanconi anemia pathway and the broader DNA damage response system. The FA pathway which involves over 20 proteins forms a network integral in repairing DNA crosslinks. FANCI tightly interacts with proteins like FANCD2 to initiate DNA repair in response to damage. The successful coordination of these proteins ensures the stability of genetic material throughout the cell cycle affecting cell fate decisions such as survival or apoptosis.

FANCI mutations are directly linked to Fanconi anemia a genetic disorder characterized by bone marrow failure and increased cancer risk. Mutations can impair the protein’s function causing defective DNA repair capability. Additionally FANCI's involvement in DNA repair associates it with breast cancer where malfunction or reduced expression of FANCI is observed. The protein interacts with tumor suppressors like BRCA1 and BRCA2 linking it to pathways involved in hereditary breast cancer predisposition.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA : binds both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage.

See full target information FANCI

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 48: PubMed35703356

2022

UBE2T regulates FANCI monoubiquitination to promote NSCLC progression by activating EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Jiguang Zhang,Jingdong Wang,Jincheng Wu,Jianyuan Huang,Zhaoxian Lin,Xing Lin

Nature communications 7:11091 PubMed27033104

2016

p53 downregulates the Fanconi anaemia DNA repair pathway.

Applications

Species

Unspecified reactive species

Sara Jaber,Eléonore Toufektchan,Vincent Lejour,Boris Bardot,Franck Toledo

Nucleic acids research 42:5605-15 PubMed24589582

2014

A concomitant loss of dormant origins and FANCC exacerbates genome instability by impairing DNA replication fork progression.

Applications

ICC/IF

Species

Mouse

Spencer W Luebben,Tsuyoshi Kawabata,Charles S Johnson,M Gerard O'Sullivan,Naoko Shima

Nucleic acids research 41:10283-97 PubMed24005041

2013

Helq acts in parallel to Fancc to suppress replication-associated genome instability.

Applications

Unspecified application

Species

Unspecified reactive species

Spencer W Luebben,Tsuyoshi Kawabata,Monica K Akre,Wai Long Lee,Charles S Johnson,M Gerard O'Sullivan,Naoko Shima

PloS one 8:e56955 PubMed23451117

2013

Loss of GGN leads to pre-implantation embryonic lethality and compromised male meiotic DNA double strand break repair in the mouse.

Applications

Species

Mouse

Duangporn Jamsai,Anne E O'Connor,Kathleen D Deboer,Brett J Clark,Stephanie J Smith,Catherine M Browne,Jonathan G Bensley,Julie A Merriman,Wai Shan Yuen,Peter Koopman,Keith T Jones,Moira K O'Bryan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FANCI antibody

Immunoprecipitation - Anti-FANCI antibody (AB74332)

Western blot - Anti-FANCI antibody (AB74332)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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