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AB228518

Anti-FANCI (phospho S559) antibody

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(1 Review)

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(1 Publication)

Rabbit Polyclonal FANCI phospho S559 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FANCI phospho S559.

View Alternative Names

KIAA1794, FANCI, Fanconi anemia group I protein, Protein FACI

2 Images
Western blot - Anti-FANCI (phospho S559) antibody (AB228518)
  • WB

Supplier Data

Western blot - Anti-FANCI (phospho S559) antibody (AB228518)

All lanes:

Western blot - Anti-FANCI (phospho S559) antibody (ab228518) at 1/250 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate treated with UV (~254 nm) for 20 minutes

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate treated with UV (~254 nm) for 20 minutes followed by treatment with lambda phosphatase (1200 units for 30 min)

Predicted band size: 149 kDa

false

Western blot - Anti-FANCI (phospho S559) antibody (AB228518)
  • WB

Supplier Data

Western blot - Anti-FANCI (phospho S559) antibody (AB228518)

All lanes:

Western blot - Anti-FANCI (phospho S559) antibody (ab228518) at 1/250 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate treated with UV (~254 nm) for 0 minutes

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate treated with UV (~254 nm) for 20 minutes

Predicted band size: 149 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human FANCI phospho S559. The exact immunogen used to generate this antibody is proprietary information.

Q9NVI1

Specificity

Specific for endogenous levels of the ~150 kDa FANCI protein phosphorylated at Ser559. Immunolabeling is increased when HeLa cells are treated with 256nm UV light for 20 minutes and completely eliminated by treatment with lambda phosphatase.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab228518 is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Storage buffer
pH: 7.5 Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.24% HEPES, 0.01% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FANCI also known as Fanconi anemia group I protein is an important player in DNA repair processes. This protein has a molecular mass of approximately 150 kDa. It is ubiquitously expressed with notable levels found in tissues that undergo high rates of cell division. FANCI participates mechanically within the DNA repair mechanism by interacting directly with DNA alongside its partner protein FANCD2 to form a tight complex that recognizes and binds to sites of DNA damage.
Biological function summary

The FANCI protein acts as part of the multiprotein Fanconi anemia (FA) core complex. This complex Functions in the repair of DNA interstrand crosslinks which are severe forms of DNA damage. Successful function of FANCI is important for maintaining genomic stability and subsequently proper cell cycle progression. It partners with proteins including FANCD2 to coordinate the homologous recombination repair process. Defective FANCI leads to disrupted DNA repair resulting in cellular damage and apoptosis.

Pathways

FANCI plays a major role in the Fanconi anemia pathway and the broader DNA damage response system. The FA pathway which involves over 20 proteins forms a network integral in repairing DNA crosslinks. FANCI tightly interacts with proteins like FANCD2 to initiate DNA repair in response to damage. The successful coordination of these proteins ensures the stability of genetic material throughout the cell cycle affecting cell fate decisions such as survival or apoptosis.

FANCI mutations are directly linked to Fanconi anemia a genetic disorder characterized by bone marrow failure and increased cancer risk. Mutations can impair the protein’s function causing defective DNA repair capability. Additionally FANCI's involvement in DNA repair associates it with breast cancer where malfunction or reduced expression of FANCI is observed. The protein interacts with tumor suppressors like BRCA1 and BRCA2 linking it to pathways involved in hereditary breast cancer predisposition.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA : binds both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage.
See full target information FANCI phospho S559
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 114:855-869 PubMed36403197

2022

TTI1 promotes non-small-cell lung cancer progression by regulating the mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Xian Zhang,Xin Yang,Zhi-Bo Wu,Zhong-Min Liao,Ding-Guo Wang,Shi-Wei Chen,Feng Lu,Yong-Bing Wu,Shu-Qiang Zhu
View all publications
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