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AB272618

Anti-FANCL antibody

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Rabbit Polyclonal FANCL antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FANCL aa 50-200.

View Alternative Names

PHF9, FANCL, E3 ubiquitin-protein ligase FANCL, Fanconi anemia group L protein, Fanconi anemia-associated polypeptide of 43 kDa, RING-type E3 ubiquitin transferase FANCL, FAAP43

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCL antibody (AB272618)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCL antibody (AB272618)

Paraffin-embedded formalin-fixed Human duodenum tissue stained for FANCL using ab272618 at 1/50 in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-FANCL antibody (AB272618)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FANCL antibody (AB272618)

PFA fixed, Triton X-100 permeablized human cell line U-2 OS labeling FANCL (green) using ab272618at 2 ug/ml in ICC/IF analysis.

Western blot - Anti-FANCL antibody (AB272618)
  • WB

Unknown

Western blot - Anti-FANCL antibody (AB272618)

All lanes:

Western blot - Anti-FANCL antibody (ab272618) at 0.4 µg/mL

All lanes:

NTERA-2

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human FANCL aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q9NW38

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Complementary Products

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Primary Antibodies

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Primary Antibodies

AB76012

Anti-Rad17 (phospho S656) antibody [EPR3145]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad17 (phospho S656) antibody [EPR3145] (AB76012)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FANCL also known as Fanconi anemia group L protein participates in the Fanconi anemia (FA) pathway. It has a molecular mass of about 42 kDa. FANCL expresses in various tissues with notable expression in the bone marrow and blood-forming organs. The protein works mechanically by functioning as an E3 ubiquitin ligase essential for monoubiquitination of FANCD2 and FANCI which is an important step in the FA DNA repair pathway.
Biological function summary

This protein plays a role in DNA damage repair. FANCL is part of a multiprotein FA complex which is responsible for the repair of DNA interstrand crosslinks. The FA complex coordinates the activation and repair processes required to maintain genome stability. It ensures proper cell cycle progression while responding to DNA damage facilitating normal cellular function and development.

Pathways

FANCL is a component of the FA-BRCA pathway and has strong connections with DNA repair processes. This pathway includes interactions with proteins such as BRCA1 and BRCA2 which manage DNA repair to prevent tumorigenesis. FANCL also engages with elements of the homologous recombination repair pathway where it ensures proper DNA repair by managing the ubiquitination processes.

Defects in FANCL relate to Fanconi anemia a genetic disorder marked by bone marrow failure and cancer predisposition. The protein's association with BRCA2 also links to hereditary breast and ovarian cancers. These connections highlight FANCL's significance in maintaining genetic integrity and its potential impact on cancer development when impaired.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin ligase protein that mediates monoubiquitination of FANCD2 in the presence of UBE2T, a key step in the DNA damage pathway (PubMed : 12973351, PubMed : 16916645, PubMed : 17938197, PubMed : 19111657, PubMed : 24389026). Also mediates monoubiquitination of FANCI (PubMed : 19589784). May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.
See full target information FANCL
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Product promise

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