Rabbit Multiclonal Fibroblast activation protein, alpha antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-FAP antibody [RM1080] - BSA and Azide free (AB314457), expandable thumbnail](https://content.abcam.com/products/images/fap-antibody-rm1080-bsa-and-azide-free-ab314457--immunocytochemistry-immunofluorescence-img421150.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-FAP antibody [RM1080] - BSA and Azide free (AB314457)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAP antibody [RM1080] - BSA and Azide free (AB314457), expandable thumbnail](https://content.abcam.com/products/images/fap-antibody-rm1080-bsa-and-azide-free-ab314457--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421127.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAP antibody [RM1080] - BSA and Azide free (AB314457)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAP antibody [RM1080] - BSA and Azide free (AB314457), expandable thumbnail](https://content.abcam.com/products/images/fap-antibody-rm1080-bsa-and-azide-free-ab314457--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421126.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAP antibody [RM1080] - BSA and Azide free (AB314457)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAP antibody [RM1080] - BSA and Azide free (AB314457), expandable thumbnail](https://content.abcam.com/products/images/fap-antibody-rm1080-bsa-and-azide-free-ab314457--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421125.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAP antibody [RM1080] - BSA and Azide free (AB314457)")
![Western blot - Anti-FAP antibody [RM1080] - BSA and Azide free (AB314457), expandable thumbnail](https://content.abcam.com/products/images/fap-antibody-rm1080-bsa-and-azide-free-ab314457--western-blot-img421045.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FAP antibody [RM1080] - BSA and Azide free (AB314457)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
The protein expressed by the FAP gene is a cell surface glycoprotein serine protease involved in the degradation of the extracellular matrix (ECM) and various cellular processes such as tissue remodeling, fibrosis, wound healing, inflammation, and tumor growth. It exists in both plasma membrane-bound and soluble forms, exhibiting post-proline cleaving endopeptidase activity, specifically targeting Ala/Ser-Gly-Pro-Ser/Asn/Ala sequences in substrates like alpha-2-antiplasmin SERPINF2 and SPRY2. FAP protein degrades gelatin and heat-denatured type I collagen but not native type I and IV collagens, nor vitronectin, tenascin, laminin, fibronectin, fibrin, or casein. It also has dipeptidyl peptidase activity, hydrolyzing prolyl bonds when the penultimate residue is proline, with a preference for sequences such as Ala-Pro and Gly-Pro. It acts on natural neuropeptides like neuropeptide Y, peptide YY, substance P, and brain natriuretic peptide 32. In its membrane form, in association with DPP4, PLAUR, or integrins, FAP is involved in pericellular ECM proteolysis, promoting cell adhesion, migration, invasion, and plays roles in development, wound healing, and cell invasiveness in malignant melanoma. It supports tumor progression by facilitating angiogenesis, collagen degradation, apoptosis, and reducing immune response. Additionally, it promotes glioma cell invasion by degrading brevican and functions as a tumor suppressor in melanocytic cells by regulating cell proliferation and survival independently of its serine protease activity. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
Prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, Fibroblast activation protein alpha, Gelatine degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, Surface-expressed protease, FAPalpha, SIMP, Seprase, FAP
Recommended products
Rabbit Multiclonal Fibroblast activation protein, alpha antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- RM1080
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab314457 is the carrier-free version of Anti-FAP antibody [RM1080] ab314456.
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FAP also known as fibroblast activation protein alpha or seprase is a serine protease with a mass of approximately 97 kDa. It is largely expressed in embryonic tissues certain carcinomas and tissue remodeling sites. FAP acts mechanistically by exhibiting dipeptidyl peptidase and endopeptidase activities breaking down peptide chains. Cellular membrane-bound FAP localizes mainly on reactive stromal fibroblasts not normal tissues making it an interesting target for research.
- Biological function summary
FAP plays a role in tissue remodeling and wound healing influencing the microenvironment of tumors and fibrotic tissues. It contributes to extracellular matrix degradation participating in the complex network of proteases. FAP is not a standalone unit but part of a larger complex that interacts with other proteases helping in cell migration and invasion. As a result its activity impacts processes like inflammation and carcinogenesis.
- Pathways
FAP participates in processes related to tissue repair and cancer progression. Significant pathways include the degradation of the extracellular matrix and the PI3K/AKT signaling. It functions alongside proteins like DPP4 another dipeptidyl peptidase in these pathways. The matrix breakdown is central to cancer metastasis and wound healing enhancing our understanding of FAP's role in larger biological contexts.
- Associated diseases and disorders
FAP has links with cancer and fibrosis. Its expression correlates with aggressive tumors and poor prognosis in cancers such as breast and ovarian. In fibrotic diseases like pulmonary and liver fibrosis it contributes to excessive matrix deposition and tissue stiffening. FAP's involvement alongside collagenases in these disorders makes it a target of potential therapeutic interest.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-FAP antibody [RM1080] - BSA and Azide free (ab314457), expandable thumbnail](https://content.abcam.com/products/images/fap-antibody-rm1080-bsa-and-azide-free-ab314457--immunocytochemistry-immunofluorescence-img421150.jpg "Immunocytochemistry/ Immunofluorescence - Anti-FAP antibody [RM1080] - BSA and Azide free (ab314457)")
Immunocytochemistry/ Immunofluorescence - Anti-FAP antibody [RM1080] - BSA and Azide free (ab314457)
This data was developed using Anti-FAP antibody [RM1080] ab314456, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized WI-38 (human fetal lung fibroblast cell) cells labelling FAP with Anti-FAP antibody [RM1080] ab314456 at 1/100 (5.37 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in WI-38 cell line. Negative control: Jurkat.Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAP antibody [RM1080] - BSA and Azide free (ab314457), expandable thumbnail](https://content.abcam.com/products/images/fap-antibody-rm1080-bsa-and-azide-free-ab314457--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421127.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAP antibody [RM1080] - BSA and Azide free (ab314457)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAP antibody [RM1080] - BSA and Azide free (ab314457)
This data was developed using Anti-FAP antibody [RM1080] ab314456, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling FAP with Anti-FAP antibody [RM1080] ab314456 at 1/1000 (0.537 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human liver. The section was incubated with Anti-FAP antibody [RM1080] ab314456 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAP antibody [RM1080] - BSA and Azide free (ab314457), expandable thumbnail](https://content.abcam.com/products/images/fap-antibody-rm1080-bsa-and-azide-free-ab314457--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421126.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAP antibody [RM1080] - BSA and Azide free (ab314457)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAP antibody [RM1080] - BSA and Azide free (ab314457)
This data was developed using Anti-FAP antibody [RM1080] ab314456, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue labeling FAP with Anti-FAP antibody [RM1080] ab314456 at 1/1000 (0.537 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the stroma in human lung carcinoma. The section was incubated with Anti-FAP antibody [RM1080] ab314456 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAP antibody [RM1080] - BSA and Azide free (ab314457), expandable thumbnail](https://content.abcam.com/products/images/fap-antibody-rm1080-bsa-and-azide-free-ab314457--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img421125.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAP antibody [RM1080] - BSA and Azide free (ab314457)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAP antibody [RM1080] - BSA and Azide free (ab314457)
This data was developed using Anti-FAP antibody [RM1080] ab314456, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling FAP with Anti-FAP antibody [RM1080] ab314456 at 1/1000 (0.537 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the stroma in human colon carcinoma. The section was incubated with Anti-FAP antibody [RM1080] ab314456 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.![Western blot - Anti-FAP antibody [RM1080] - BSA and Azide free (ab314457), expandable thumbnail](https://content.abcam.com/products/images/fap-antibody-rm1080-bsa-and-azide-free-ab314457--western-blot-img421045.jpg "Western blot - Anti-FAP antibody [RM1080] - BSA and Azide free (ab314457)")
Western blot - Anti-FAP antibody [RM1080] - BSA and Azide free (ab314457)
This data was developed using Anti-FAP antibody [RM1080] ab314456, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Jurkat.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.All lanes: Western blot - Anti-FAP antibody [RM1080] (Anti-FAP antibody [RM1080] ab314456) at 1/1000 dilution
Lane 1: HFF-1 (human skin fibroblast) whole cell lysate at 20 µg
Lane 2: IMR-90 (human lung fibroblast) whole cell lysate at 20 µg
Lane 3: WI-38 (human fetal lung fibroblast) whole cell lysate at 20 µg
Lane 4: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 95 kDa
Exposure time: 37s
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