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AB224049

Anti-FARSLA antibody

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(1 Publication)

Rabbit Polyclonal FARSLA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FARSA aa 1-200.

View Alternative Names

FARS, FARSL, FARSLA, FARSA, Phenylalanine--tRNA ligase alpha subunit, CML33, Phenylalanyl-tRNA synthetase alpha subunit, PheRS

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-FARSLA antibody (AB224049)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FARSLA antibody (AB224049)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for FARSLA (green) using ab224049 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FARSLA antibody (AB224049)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FARSLA antibody (AB224049)

Paraffin-embedded human duodenum tissue stained for FARSLA using ab224049 at 1/1000 dilution in immunohistochemical analysis.

Western blot - Anti-FARSLA antibody (AB224049)
  • WB

Supplier Data

Western blot - Anti-FARSLA antibody (AB224049)

All lanes:

Western blot - Anti-FARSLA antibody (ab224049) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 58 kDa,75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FARSA aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9Y285

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FARSLA protein also known as phenylalanyl-tRNA synthetase alpha subunit is an essential enzyme in protein synthesis. This protein has a molecular mass of approximately 58 kDa. It is part of the aminoacyl-tRNA synthetase family and catalyzes the attachment of phenylalanine to its corresponding tRNA a critical step in translating mRNA into proteins. FARSLA is expressed widely across various tissues reflecting its fundamental role in cellular function.
Biological function summary

FARSLA is involved in producing and maintaining the accurate genetic code translation. It operates as part of the heterotetrameric phenylalanyl-tRNA synthetase complex which includes another subunit. This enzyme ensures that phenylalanine is correctly linked to tRNA with its specific anticodon facilitating the incorporation of phenylalanine into growing polypeptide chains during ribosomal assembly.

Pathways

FARSLA functions in the aminoacyl-tRNA biosynthesis pathway a central process required for efficient protein production. This pathway is fundamental to the genetic translation machinery making it essential for cellular life. FARSLA interacts with other aminoacyl-tRNA synthetases like leucyl-tRNA synthetase ensuring high-fidelity translation by linking correct amino acids to their respective tRNA molecules.

FARSLA has potential connections to conditions affecting protein synthesis and cellular growth. Mutations or dysregulation of FARSLA are linked to Charcot-Marie-Tooth disease a disorder that impacts peripheral nerves and muscle function. Another related disorder is infantile liver failure syndrome 2 which connects FARSLA with liver dysfunction and metabolic complications. These conditions may involve interactions with other proteins such as tyrosyl-tRNA synthetase highlighting the complex interplay of aminoacyl-tRNA synthetases in human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information FARSA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:3970 PubMed34172739

2021

The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bijeta Prasai,Gideon J Haber,Marie-Paule Strub,Regina Ahn,John A Ciemniecki,Kem A Sochacki,Justin W Taraska
View all publications
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Product promise

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