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AB185766

Anti-Fas antibody [EOS9.1] - Low endotoxin, Azide free

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Mouse Monoclonal Fas antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD95, APT1, FAS1, TNFRSF6, FAS, Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor

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Flow Cytometry - Anti-Fas antibody [EOS9.1] - Low endotoxin, Azide free (AB185766)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fas antibody [EOS9.1] - Low endotoxin, Azide free (AB185766)

Flow cytometric analysis of unstimulated Jurkat cells (left), or Jukar cells stimulated with 5 ug/mL ab185766 (right). The stimulated cells were stianed with Fixable Viability Dye eFluor® 780 and Annexin V Apoptosis Detection Kit eFluor® 450 then fixed and permeabilized with the Foxp3 Staining Buffer Set, and stained with Anti-Human PARP1 (Cleaved) PE. Total viable cells (Fixable Viability Dye eFluor® 780 negative) were used for analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

EOS9.1

Isotype

IgM

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>1 μg/test</p>" } } }

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

Product details

Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by the LAL assay.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
See full target information FAS

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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