AB185766

Anti-Fas antibody [EOS9.1] - Low endotoxin, Azide free

Name: Anti-Fas antibody [EOS9.1] - Low endotoxin, Azide free (ab185766) | Abcam
Brand: Abcam Limited
SKU: AB185766
Price: 460 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Fas antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD95, APT1, FAS1, TNFRSF6, FAS, Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor

1 Images

Flow Cytometry - Anti-Fas antibody [EOS9.1] - Low endotoxin, Azide free (AB185766)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fas antibody [EOS9.1] - Low endotoxin, Azide free (AB185766)

Flow cytometric analysis of unstimulated Jurkat cells (left), or Jukar cells stimulated with 5 ug/mL ab185766 (right). The stimulated cells were stianed with Fixable Viability Dye eFluor® 780 and Annexin V Apoptosis Detection Kit eFluor® 450 then fixed and permeabilized with the Foxp3 Staining Buffer Set, and stained with Anti-Human PARP1 (Cleaved) PE. Total viable cells (Fixable Viability Dye eFluor® 780 negative) were used for analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

EOS9.1

Isotype

IgM

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>1 μg/test</p>" } } }

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Product details

Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by the LAL assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).

See full target information FAS

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 12:e0186593 PubMed29040321

2017

Co-delivery of micronized urinary bladder matrix damps regenerative capacity of minced muscle grafts in the treatment of volumetric muscle loss injuries.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen M Goldman,Benjamin T Corona

View all publications

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Product promise

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