Anti-Fas antibody [EPR24898-74] (ab271016)

Anti-Fas antibody [EPR24898-74] is a Rabbit Monoclonal antibody that is used in Fas IHC-Fr, IHC-P, IP, Western Blot. Suitable for Mouse samples.

FAS, also known as CD95 or APO-1, is a cell surface receptor that plays a significant role in regulating apoptosis (programmed cell death). FAS is crucial for inducing apoptosis in cancer cells. When FAS binds to its ligand (FASL), it triggers a cascade leading to cell death, which is essential for eliminating cancer cells.FAS was identified as an independent prognostic marker for recurrence free survival in breast cancer, with large variation in expression by receptor subtypes. Interestingly, the prognostic effect of CD8+ TILs in ER-negative disease was only valid for tumors with a high FAS expression.FAS expression might therefore act as a predictive factor for new emerging therapies.Researchers are exploring ways to enhance FAS signaling to prevent cancer relapse and improve the durability of immunotherapy responses.

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Images

Immunoprecipitation - Anti-Fas antibody [EPR24898-74] (AB271016), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-P	IHC-Fr	Flow Cyt (Intra)
Human	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Mouse	Tested	Tested	Tested	Tested	Not recommended
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information Fas

Function

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity).

Alternative names

CD95, Apt1, Tnfrsf6, Fas, Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR24898-74
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Immunoprecipitation - Anti-Fas antibody [EPR24898-74] (ab271016)
Fas was immunoprecipitated from mouse thymus tissue lysate with ab271016 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed from innunoprecipitate using ab271016 at 1/1000 dilution. Secondary antibody VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution.
Lane 1: Mouse thymus tissue lysate (Input) 10 μg
Lane 2: ab271016 IP in Mouse thymus tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab271016 in mouse thymus tissue lysate
Exposure time: 5.5 seconds
The 20KDa band could be a degradation or cleavage product, as demonstrated by the use of fresh lysate in the WB data.
All lanes: Immunoprecipitation - Anti-Fas antibody [EPR24898-74] (ab271016)
Predicted band size: 37 kDa
Observed band size: 45 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [EPR24898-74] (ab271016)
Immunohistochemical analysis of paraffin-embedded sections of mouse thymus labelling Fas with ab271016 at 1/100 dilution followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse thymus. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. The section was incubated with ab271016 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: PBS instead of primary antibody followed by LeicaDS9800 (Bond™ Polymer Refine Detection) as a secondary antibody.
Immunohistochemistry (Frozen sections) - Anti-Fas antibody [EPR24898-74] (ab271016)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse thymus (fresh) tissue labelling Fas with ab 271016 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution (Green). Positive staining on mouse thymus. The nuclear counter stain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody follwed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [EPR24898-74] (ab271016)
Immunohistochemical analysis of paraffin-embedded sections of mouse lung cancer labelling Fas with ab271016 at 1/100 dilution followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse lung cancer tissue. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. The section was incubated with ab271016 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: PBS instead of primary antibody followed by LeicaDS9800 (Bond™ Polymer Refine Detection) as a secondary antibody.
Western blot - Anti-Fas antibody [EPR24898-74] (ab271016)
5% NFDM/TBST was used as blocking and diluting buffer.
The observed MW are consistent with what has been described in the literature (PMID: 28883393).
Low expression: stomach, skin (PMID: 31582729; PMID: 11106570).
All lanes: Western blot - Anti-Fas antibody [EPR24898-74] (ab271016) at 1/1000 dilution
Lane 1: Mouse spleen tissue lysate at 20 µg
Lane 2: Mouse stomach tissue lysate at 20 µg
Lane 3: Mouse skin tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 37 kDa
Observed band size: 45 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [EPR24898-74] (ab271016)
Immunohistochemical analysis of paraffin-embedded sections of mouse spleen labelling Fas with ab271016 at 1/100 dilution followed by ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse spleen. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. The section was incubated with ab271016 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Secondary antibody only control: PBS instead of primary antibody followed by LeicaDS9800 (Bond™ Polymer Refine Detection) as a secondary antibody.
Western blot - Anti-Fas antibody [EPR24898-74] (ab271016)
5% NFDM/TBST was used as a blocking and diluting buffer.
Exposure times:
Lane 1-2 : 37 seconds;
Lane 3 : 81 seconds.
Lanes 2-3 lysate was made fresh and used immediately to minimize protein degradation.
The 20 kDa band in lane 1 could be a degradation or cleavage product.
All lanes: Western blot - Anti-Fas antibody [EPR24898-74] (ab271016) at 1/1000 dilution
Lane 1: Mouse thymus tissue lysate at 20 µg
Lane 2: Mouse thymus tissue fresh lysate at 20 µg
Lane 3: A20 (mouse reticulum sarcoma B lymphocyte) whole cell fresh lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 37 kDa
Observed band size: 45 kDa
Western blot - Anti-Fas antibody [EPR24898-74] (ab271016)
5% NFDM/TBST was used as a blocking and diluting buffer.
Exposure times:
Lane 1, 3: 26 seconds;
Lane 2 : 136 seconds.
The observed MW are consistent with what has been described in the literature (PMID: 28883393).
All lanes: Western blot - Anti-Fas antibody [EPR24898-74] (ab271016) at 1/1000 dilution
Lane 1: Mouse liver tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Mouse lung tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 45 kDa
Immunohistochemistry (Frozen sections) - Anti-Fas antibody [EPR24898-74] (ab271016)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labelling Fas with ab 271016 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution (Green). Positive staining on mouse liver. The nuclear counter stain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution.
Western blot - Anti-Fas antibody [EPR24898-74] (ab271016)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Fas antibody [EPR24898-74] (ab271016) at 1/1000 dilution
Lane 1: Mouse pancreas tissue lysate at 20 µg
Lane 2: Mouse placenta tissue lysate at 20 µg
Lane 3: Mouse uterus tissue lysate at 20 µg
Lane 4: J774A.1 (mouse reticulum cell sarcoma monocyte macrophage) whole cell lysate at 20 µg
Lane 5: L929 (mouse connective tissue fibroblast) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 45 kDa
Exposure time: 180s