Mouse Monoclonal Fas antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FAS aa 1-100.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [FAS/3112] - BSA and Azide free (AB269685), expandable thumbnail](https://content.abcam.com/products/images/fas-antibody-fas-3112-bsa-and-azide-free-ab269685--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127702.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [FAS/3112] - BSA and Azide free (AB269685)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [FAS/3112] - BSA and Azide free (AB269685), expandable thumbnail](https://content.abcam.com/products/images/fas-antibody-fas-3112-bsa-and-azide-free-ab269685--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19757.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [FAS/3112] - BSA and Azide free (AB269685)")
![Protein Array - Anti-Fas antibody [FAS/3112] - BSA and Azide free (AB269685), expandable thumbnail](https://content.abcam.com/products/images/fas-antibody-fas-3112-bsa-and-azide-free-ab269685--protein-array-img1376.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Fas antibody [FAS/3112] - BSA and Azide free (AB269685)")
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human FAS aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P25445
Reactivity data
Select an application| Protein Array | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 product for Alternative Version
5 products for Alternative Product
Target data
Function
Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Alternative names
CD95, APT1, FAS1, TNFRSF6, FAS, Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor
Recommended products
Mouse Monoclonal Fas antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FAS aa 1-100.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human FAS aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P25445
- Carrier free
- Yes
- Clone number
- FAS/3112
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab269685 is the carrier-free version of Anti-Fas antibody [FAS/3112] ab269675.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [FAS/3112] - BSA and Azide free (ab269685), expandable thumbnail](https://content.abcam.com/products/images/fas-antibody-fas-3112-bsa-and-azide-free-ab269685--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127702.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [FAS/3112] - BSA and Azide free (ab269685)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [FAS/3112] - BSA and Azide free (ab269685)
Formalin-fixed, paraffin-embedded human colon tissue stained for Fas using Anti-Fas antibody [FAS/3112] ab269675 at 2 μg/ml in immunohistochemical analysis.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Fas antibody [FAS/3112] ab269675).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [FAS/3112] - BSA and Azide free (ab269685), expandable thumbnail](https://content.abcam.com/products/images/fas-antibody-fas-3112-bsa-and-azide-free-ab269685--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img19757.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [FAS/3112] - BSA and Azide free (ab269685)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [FAS/3112] - BSA and Azide free (ab269685)
Formalin-fixed, paraffin-embedded human colon tissue stained for Fas using Anti-Fas antibody [FAS/3112] ab269675 at 2 μg/ml in immunohistochemical analysis.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Fas antibody [FAS/3112] ab269675).
![Protein Array - Anti-Fas antibody [FAS/3112] - BSA and Azide free (ab269685), expandable thumbnail](https://content.abcam.com/products/images/fas-antibody-fas-3112-bsa-and-azide-free-ab269685--protein-array-img1376.jpg "Protein Array - Anti-Fas antibody [FAS/3112] - BSA and Azide free (ab269685)")
Protein Array - Anti-Fas antibody [FAS/3112] - BSA and Azide free (ab269685)
Analysis of Protein Array containing more than 19,000 full-length human proteins using Anti-Fas antibody [FAS/3112] ab269675.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was deveolped using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Fas antibody [FAS/3112] ab269675).
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