AB289714

Anti-Fas antibody [LOB3/17] - BSA and Azide free

Recombinant
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Mouse Recombinant Monoclonal Fas antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD95, APT1, FAS1, TNFRSF6, FAS, Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor

3 Images

Flow Cytometry - Anti-Fas antibody [LOB3/17] - BSA and Azide free (AB289714)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fas antibody [LOB3/17] - BSA and Azide free (AB289714)

This data was developed using ab289713, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Fas with ab289713 at 1/1000 dilution (Red) compared with a Mouse monoclonal IgG (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Secondary antibody details Goat Anti-Mouse IgG (Alexa Fluor^® 647, ab150119), at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fas antibody [LOB3/17] - BSA and Azide free (AB289714)

This data was developed using ab289713, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of K-562 (Human chronic myelogenous leukemia lymphoblast, Left) / Raji(Human Burkitt's lymphoma B lymphocyte, Right) cells labeling Fas with ab289713 at 1/1000 dilution (Red) compared with a Mouse monoclonal IgG (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Mouse IgG (Alexa Fluor^® 647, ab150119), at 1/2000 dilution was used as the secondary antibody.

Negative control : K-562.
Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fas antibody [LOB3/17] - BSA and Azide free (AB289714)

This data was developed using ab289713, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling Fas with ab289713 at 1/1000 dilution (right), compared with a Mouse monoclonal IgG (left). Secondary antibody details Goat Anti-Mouse IgG (Alexa Fluor^® 488, ab150113), at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LOB 3/17

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

This antibody is a carrier free version of ab287913.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).

See full target information FAS

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fas antibody [LOB3/17] - BSA and Azide free

Flow Cytometry - Anti-Fas antibody [LOB3/17] - BSA and Azide free (AB289714)

Flow Cytometry - Anti-Fas antibody [LOB3/17] - BSA and Azide free (AB289714)

Flow Cytometry - Anti-Fas antibody [LOB3/17] - BSA and Azide free (AB289714)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise