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AB272361

Anti-Fas Ligand antibody [EPR22516-218] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Fas Ligand antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

View Alternative Names

CD178, Apt1lg1, Cd95l, Fasl, gld, Tnfsf6, Faslg, Tumor necrosis factor ligand superfamily member 6, CD95 ligand, Fas antigen ligand, CD95-L, Fas ligand, FasL

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Sandwich ELISA - Anti-Fas Ligand antibody [EPR22516-218] - BSA and Azide free (Detector) (AB272361)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Fas Ligand antibody [EPR22516-218] - BSA and Azide free (Detector) (AB272361)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab270894).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22516-218

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab272361 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Fas Ligand. The recommended pair for sandwich ELISA is:
Capture: ab272360, Mouse Fas Ligand Capture Antibody (unconjugated)
Detector: ab272361, Mouse Fas Ligand Detector Antibody (unconjugated)
The reference range value is 18.75 - 1200 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed : 7511063). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed : 19794494, PubMed : 7532682). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (PubMed : 19794494). TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (PubMed : 10779162). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (By similarity).. Tumor necrosis factor ligand superfamily member 6, soluble form. Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways (PubMed : 19794494). Can induce apoptosis but does not appear to be essential for this process (By similarity).. FasL intracellular domain. Cytoplasmic form induces gene transcription inhibition.
See full target information Faslg
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Complementary Products

Assay Kits

AB270894

Mouse Fas Ligand ELISA Kit (FasL)

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Primary Antibodies

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com