Rabbit Recombinant Monoclonal Fas Ligand antibody. Carrier free. Suitable for WB, IP and reacts with Human samples.
Images
![Western blot - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (AB302906), expandable thumbnail](https://content.abcam.com/products/images/fas-ligand-antibody-epr25843-87-bsa-and-azide-free-ab302906--western-blot-img445016.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (AB302906)")
![Western blot - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (AB302906), expandable thumbnail](https://content.abcam.com/products/images/fas-ligand-antibody-epr25843-87-bsa-and-azide-free-ab302906--western-blot-img445017.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (AB302906)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:26334989, PubMed:9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:7528780, PubMed:9228058, PubMed:9427603). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity). TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed:27806260). Tumor necrosis factor ligand superfamily member 6, soluble form. Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways (By similarity). Can induce apoptosis but does not appear to be essential for this process (PubMed:27806260). FasL intracellular domain. Cytoplasmic form induces gene transcription inhibition.
Alternative names
CD178, APT1LG1, CD95L, FASL, TNFSF6, FASLG, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, CD95 ligand, Fas antigen ligand, APTL, CD95-L, Fas ligand, FasL
Recommended products
Rabbit Recombinant Monoclonal Fas Ligand antibody. Carrier free. Suitable for WB, IP and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25843-87
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (ab302906), expandable thumbnail](https://content.abcam.com/products/images/fas-ligand-antibody-epr25843-87-bsa-and-azide-free-ab302906--western-blot-img445016.jpg "Western blot - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (ab302906)")
Western blot - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (ab302906)
This data was developed using Anti-Fas Ligand antibody [EPR25843-87] ab302905, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-Fas Ligand antibody [EPR25843-87] (Anti-Fas Ligand antibody [EPR25843-87] ab302905) at 1/1000 dilution
Lane 1: Untreated No-GFP-CD16.NK-92 (natural killer (NK-92®) cell lacking GFP-CD16) whole cell lysate at 40 µg
Lane 2: No-GFP-CD16.NK-92 treated with 80nM TPA (Phorbol 12-myristate 13-acetate (PMA), PKC activator ab120297) and 3µM Ionomycin (Ionomycin Ca2+ Salt, Ca2+ ionophore ab120116) for 5 hours, was then added for an additional 300ng/ml BFA for last 4 hours whole cell lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 31 kDa
Observed band size: 37 kDa
Exposure time: 180s
![Western blot - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (ab302906), expandable thumbnail](https://content.abcam.com/products/images/fas-ligand-antibody-epr25843-87-bsa-and-azide-free-ab302906--western-blot-img445017.jpg "Western blot - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (ab302906)")
Western blot - Anti-Fas Ligand antibody [EPR25843-87] (BSA and Azide free) (ab302906)
This data was developed using Anti-Fas Ligand antibody [EPR25843-87] ab302905, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-Fas Ligand antibody [EPR25843-87] (Anti-Fas Ligand antibody [EPR25843-87] ab302905) at 1/1000 dilution
Lane 1: Untreated Jurkat (human T cell leukemia T lymphocyte from peripheral blood), whole cell lysate at 40 µg
Lane 2: Jurkat treated with 80nM TPA (Phorbol 12-myristate 13-acetate (PMA), PKC activator ab120297) and 3µM Ionomycin (Ionomycin Ca2+ Salt, Ca2+ ionophore ab120116) for 5 hours, was then added for an additional 300ng/ml BFA for last 4 hours, whole cell lysate at 40 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 31 kDa
Observed band size: 37 kDa
Exposure time: 180s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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