Mouse Recombinant Monoclonal Fas Ligand antibody. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-Fas Ligand antibody [G247-4] (AB303666), expandable thumbnail](https://content.abcam.com/products/images/fas-ligand-antibody-g247-4-ab303666--western-blot-img445182.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fas Ligand antibody [G247-4] (AB303666)")
![Western blot - Anti-Fas Ligand antibody [G247-4] (AB303666), expandable thumbnail](https://content.abcam.com/products/images/fas-ligand-antibody-g247-4-ab303666--western-blot-img404533.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fas Ligand antibody [G247-4] (AB303666)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | Flow Cyt | |
|---|---|---|---|
| Human | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes We recommend optimizing the protocol to enhance signal intensity by increasing protein loading (30-50 μg/lane), reducing primary antibody dilution (1:500), and utilizing femtogram-level ECL substrates. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:26334989, PubMed:9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:7528780, PubMed:9228058, PubMed:9427603). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity). TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed:27806260). Tumor necrosis factor ligand superfamily member 6, soluble form. Induces FAS-mediated activation of NF-kappa-B, initiating non-apoptotic signaling pathways (By similarity). Can induce apoptosis but does not appear to be essential for this process (PubMed:27806260). FasL intracellular domain. Cytoplasmic form induces gene transcription inhibition.
Alternative names
CD178, APT1LG1, CD95L, FASL, TNFSF6, FASLG, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, CD95 ligand, Fas antigen ligand, APTL, CD95-L, Fas ligand, FasL
Recommended products
Mouse Recombinant Monoclonal Fas Ligand antibody. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- G247- 4
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Fas Ligand antibody [G247-4] (ab303666), expandable thumbnail](https://content.abcam.com/products/images/fas-ligand-antibody-g247-4-ab303666--western-blot-img445182.jpg "Western blot - Anti-Fas Ligand antibody [G247-4] (ab303666)")
Western blot - Anti-Fas Ligand antibody [G247-4] (ab303666)
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/1000000 dilution.
We recommend optimizing the protocol to enhance signal intensity by increasing protein loading (30-50 μg/lane), reducing primary antibody dilution (1/500), and utilizing femtogram-level ECL substrates.
All lanes: Western blot - Anti-Fas Ligand antibody [G247-4] (ab303666) at 1/1000 dilution
Lanes 1 and 3: Untreated No-GFP-CD16.NK-92 (natural killer (NK-92®) cell lacking GFP-CD16) whole cell lysate at 20 µg
Lanes 2 and 4: No-GFP-CD16.NK-92 treated with 80nM TPA (Phorbol 12-myristate 13-acetate (PMA), PKC activator ab120297) and 3µM Ionomycin (Ionomycin Ca2+ Salt, Ca2+ ionophore ab120116) for 5 hours, was then added for an additional 300ng/ml BFA for last 4 hours whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Developed using the ECL technique.
Observed band size: 40 kDa
Exposure time: 40s
![Western blot - Anti-Fas Ligand antibody [G247-4] (ab303666), expandable thumbnail](https://content.abcam.com/products/images/fas-ligand-antibody-g247-4-ab303666--western-blot-img404533.jpg "Western blot - Anti-Fas Ligand antibody [G247-4] (ab303666)")
Western blot - Anti-Fas Ligand antibody [G247-4] (ab303666)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
All lanes: Western blot - Anti-Fas Ligand antibody [G247-4] (ab303666) at 1/1000 dilution
Lane 1: Untreated No-GFP-CD16.NK-92 (natural killer (NK-92®) cell lacking GFP-CD16) whole cell lysate at 30 µg
Lane 2: No-GFP-CD16.NK-92 treated with 80nM TPA (Phorbol 12-myristate 13-acetate (PMA), PKC activator ab120297) and 3µM Ionomycin (Ionomycin Ca2+ Salt, Ca2+ ionophore ab120116) for 5 hours, was then added for an additional 300ng/ml BFA for last 4 hours whole cell lysate at 20 µg
SecondaryAll lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution
Developed using the ECL technique.
Observed band size: 40 kDa
Exposure time: 3min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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