AB183891

Anti-Fascin antibody

Name: Anti-Fascin antibody (ab183891) | Abcam
Brand: Abcam Limited
SKU: AB183891

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(4 Publications)

Rabbit Polyclonal Fascin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FSCN1 aa 50-400.

View Alternative Names

FAN1, HSN, SNL, FSCN1, Fascin, 55 kDa actin-bundling protein, Singed-like protein, p55

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Fascin antibody (AB183891)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Fascin antibody (AB183891)

Immunofluorescence analysis of HCT116 cells fixed in 100% methanol for 5 minutes, labeling Fascin using ab183891 at a 1/500 dilution (green). Blue : Hoechst 33342 staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody (AB183891)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody (AB183891)

Immunohistochemical analysis of paraffin-embedded mouse ovary tissue, labeling Fascin using ab183891 at a 1/500 dilution.

Supplier Data

Western blot - Anti-Fascin antibody (AB183891)

All lanes:

Western blot - Anti-Fascin antibody (ab183891) at 1/1000 dilution

All lanes:

HeLa whole cell lysate/extract at 30 µg

Predicted band size: 54 kDa

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Lab

Western blot - Anti-Fascin antibody (AB183891)

All lanes:

Western blot - Anti-Fascin antibody (ab183891) at 1/2000 dilution

Lane 1:

WT HeLa cell extract at 30 µg

Lane 2:

Fascin Knockout HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 54 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human FSCN1 aa 50-400. The exact immunogen used to generate this antibody is proprietary information.

Q16658

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin-binding protein that contains 2 major actin binding sites (PubMed : 21685497, PubMed : 23184945). Organizes filamentous actin into parallel bundles (PubMed : 20393565, PubMed : 21685497, PubMed : 23184945). Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers (PubMed : 22155786). Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration (PubMed : 20393565, PubMed : 21685497, PubMed : 23184945). Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF (PubMed : 22155786).

See full target information FSCN1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 11:630408 PubMed33777781

2021

Oncoproteins of High-Risk HPV and EBV Cooperate to Enhance Cell Motility and Invasion of Human Breast Cancer Cells Erk1/Erk2 and β-Catenin Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Ishita Gupta,Ayesha Jabeen,Semir Vranic,Ala-Eddin Al Moustafa,Hamda Al-Thawadi

Molecules (Basel, Switzerland) 25: PubMed32947764

2020

Plant Extract Inhibits Epithelial-Mesenchymal Transition and Induces Apoptosis via HER2 Inactivation and JNK Pathway in HER2-Positive Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ayesha Jabeen,Anju Sharma,Ishita Gupta,Hadeel Kheraldine,Semir Vranic,Ala-Eddin Al Moustafa,Halema F Al Farsi

EBioMedicine 50:178-190 PubMed31727599

2019

RETRACTED: LSAMP-AS1 binds to microRNA-183-5p to suppress the progression of prostate cancer by up-regulating the tumor suppressor DCN.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Hua,Zhen Liu,Min Zhou,Yan Tian,Pei-Pei Zhao,Wen-Hai Pan,Chao-Xia Li,Xiao-Xiao Huang,Ze-Xiao Liao,Qi Xian,Bo Chen,Yue Hu,Lei Leng,Xiao-Wei Fang,Li-Na Yu

Oncology letters 10:1333-1338 PubMed26622672

2015

Role of RNA-interference-induced zinc finger protein 139 suppression in gastric cancer cell sensitivity to chemotherapeutic agents.

Applications

Species

Unspecified reactive species

Ying-Jie Hao,Yong Li,Li-Qiao Fan,Qun Zhao,Bi-Bo Tan,Zhi-Kai Jiao,Xue-Feng Zhao,Zhi-Dong Zhang,Dong Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fascin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Fascin antibody (AB183891)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody (AB183891)

Western blot - Anti-Fascin antibody (AB183891)

Western blot - Anti-Fascin antibody (AB183891)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise