Mouse Monoclonal Fascin antibody. Suitable for IHC-P, ICC, Flow Cyt, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FSCN1.
Images
![Immunocytochemistry - Anti-Fascin antibody [FSCN1/417] (AB220195), expandable thumbnail](https://content.abcam.com/products/images/fascin-antibody-fscn1-417-ab220195--immunocytochemistry-img26239.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-Fascin antibody [FSCN1/417] (AB220195)")
![Flow Cytometry - Anti-Fascin antibody [FSCN1/417] (AB220195), expandable thumbnail](https://content.abcam.com/products/images/fascin-antibody-fscn1-417-ab220195--flow-cytometry-img81948.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Fascin antibody [FSCN1/417] (AB220195)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (AB220195), expandable thumbnail](https://content.abcam.com/products/images/fascin-antibody-fscn1-417-ab220195--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82667.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (AB220195)")
![Western blot - Anti-Fascin antibody [FSCN1/417] (AB220195), expandable thumbnail](https://content.abcam.com/products/images/fascin-antibody-fscn1-417-ab220195--western-blot-img28916.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fascin antibody [FSCN1/417] (AB220195)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (AB220195), expandable thumbnail](https://content.abcam.com/products/images/fascin-antibody-fscn1-417-ab220195--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115992.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (AB220195)")
Publications
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- Oxidative medicine and cellular longevity 2021:99797072021LYAR Promotes Colorectal Cancer Progression by Upregulating Expression and Fatty Acid Metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYupeng Wu,Yu Zhou,Haiying Gao,Yajun Wang,Qingyu Cheng,Shikun Jian,Qi Ding,Wei Gu,Yanxue Yao,Jia Ma,Wenjuan Wu,Yuyun Li,Xuhui Tong,Xiaoyuan Song,Sai MaPubMed 35069968
- Molecular medicine reports 22:5282-52922020MicroRNA‑145‑5p suppresses fascin to inhibit the invasion and migration of cervical carcinoma cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShufang He et. al.PubMed 33174029
- Reproduction in domestic animals = Zuchthygiene 54:882-8912019MicroRNA-145 regulates immune cytokines via targeting FSCN1 in Staphylococcus aureus-induced mastitis in dairy cows.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhi Chen et. al.PubMed 30974481
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human FSCN1. Database link Q16658
Reactivity data
Select an application| IHC-P | ICC | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg for 106 Cells | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
1 product for Alternative Version
Target data
Function
Actin-binding protein that contains 2 major actin binding sites (PubMed:21685497, PubMed:23184945). Organizes filamentous actin into parallel bundles (PubMed:20393565, PubMed:21685497, PubMed:23184945). Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers (PubMed:22155786). Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration (PubMed:20393565, PubMed:21685497, PubMed:23184945). Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF (PubMed:22155786).
Alternative names
FAN1, HSN, SNL, FSCN1, Fascin, 55 kDa actin-bundling protein, Singed-like protein, p55
Recommended products
Mouse Monoclonal Fascin antibody. Suitable for IHC-P, ICC, Flow Cyt, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FSCN1.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human FSCN1. Database link Q16658
- Clone number
- FSCN1/417
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- kappa
- Concentration
- Purification notes
ab220195 was purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunocytochemistry - Anti-Fascin antibody [FSCN1/417] (ab220195), expandable thumbnail](https://content.abcam.com/products/images/fascin-antibody-fscn1-417-ab220195--immunocytochemistry-img26239.jpg "Immunocytochemistry - Anti-Fascin antibody [FSCN1/417] (ab220195)")
Immunocytochemistry - Anti-Fascin antibody [FSCN1/417] (ab220195)
mmunofluorescence Analysis of HeLa cells labeling Fascin with ab220195 (Green). The nuclear counterstain is Hoescht (Blue). Membrane is Phalloidin 488 (Red)
![Flow Cytometry - Anti-Fascin antibody [FSCN1/417] (ab220195), expandable thumbnail](https://content.abcam.com/products/images/fascin-antibody-fscn1-417-ab220195--flow-cytometry-img81948.jpg "Flow Cytometry - Anti-Fascin antibody [FSCN1/417] (ab220195)")
Flow Cytometry - Anti-Fascin antibody [FSCN1/417] (ab220195)
Flow cytometric analysis of PFA-fixed K562 cells using ab220195 followed by a Goat anti-Mouse IgG (blue); isotype control (red)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (ab220195), expandable thumbnail](https://content.abcam.com/products/images/fascin-antibody-fscn1-417-ab220195--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82667.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (ab220195)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (ab220195)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human Hodgkin's lymphoma tissue labeling Fascin with ab220195 at 1 μg/ml dilution.
![Western blot - Anti-Fascin antibody [FSCN1/417] (ab220195), expandable thumbnail](https://content.abcam.com/products/images/fascin-antibody-fscn1-417-ab220195--western-blot-img28916.jpg "Western blot - Anti-Fascin antibody [FSCN1/417] (ab220195)")
Western blot - Anti-Fascin antibody [FSCN1/417] (ab220195)
All lanes: Western blot - Anti-Fascin antibody [FSCN1/417] (ab220195) at 1 µg/mL
All lanes: HeLa cell lysate
Predicted band size: 54 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (ab220195), expandable thumbnail](https://content.abcam.com/products/images/fascin-antibody-fscn1-417-ab220195--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115992.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (ab220195)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (ab220195)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human ovarian carcinoma tissue labeling Fascin with ab220195 at 1 μg/ml dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (ab220195), expandable thumbnail](https://content.abcam.com/products/images/fascin-antibody-fscn1-417-ab220195--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92275.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (ab220195)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (ab220195)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human testicular carcinoma tissue labeling Fascin with ab220195 at 1 μg/ml dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (ab220195), expandable thumbnail](https://content.abcam.com/products/images/fascin-antibody-fscn1-417-ab220195--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92275.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (ab220195)")
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