AB220195

Anti-Fascin antibody [FSCN1/417]

Name: Anti-Fascin antibody [FSCN1/417] (ab220195) | Abcam
Brand: Abcam Limited
SKU: AB220195
Price: 525 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal Fascin antibody. Suitable for IHC-P, ICC, Flow Cyt, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FSCN1.

View Alternative Names

FAN1, HSN, SNL, FSCN1, Fascin, 55 kDa actin-bundling protein, Singed-like protein, p55

6 Images

Supplier Data

Immunocytochemistry - Anti-Fascin antibody [FSCN1/417] (AB220195)

mmunofluorescence Analysis of HeLa cells labeling Fascin with ab220195 (Green). The nuclear counterstain is Hoescht (Blue). Membrane is Phalloidin 488 (Red)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (AB220195)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (AB220195)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human ovarian carcinoma tissue labeling Fascin with ab220195 at 1 μg/ml dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (AB220195)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human Hodgkin's lymphoma tissue labeling Fascin with ab220195 at 1 μg/ml dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (AB220195)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human testicular carcinoma tissue labeling Fascin with ab220195 at 1 μg/ml dilution.

Flow Cytometry - Anti-Fascin antibody [FSCN1/417] (AB220195)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fascin antibody [FSCN1/417] (AB220195)

Flow cytometric analysis of PFA-fixed K562 cells using ab220195 followed by a Goat anti-Mouse IgG (blue); isotype control (red)

Supplier Data

Western blot - Anti-Fascin antibody [FSCN1/417] (AB220195)

All lanes:

Western blot - Anti-Fascin antibody [FSCN1/417] (ab220195) at 1 µg/mL

All lanes:

HeLa cell lysate

Predicted band size: 54 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

FSCN1/417

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FSCN1.

Q16658

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

ab220195 was purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin-binding protein that contains 2 major actin binding sites (PubMed : 21685497, PubMed : 23184945). Organizes filamentous actin into parallel bundles (PubMed : 20393565, PubMed : 21685497, PubMed : 23184945). Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers (PubMed : 22155786). Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration (PubMed : 20393565, PubMed : 21685497, PubMed : 23184945). Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF (PubMed : 22155786).

See full target information FSCN1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2021:9979707 PubMed35069968

2021

LYAR Promotes Colorectal Cancer Progression by Upregulating Expression and Fatty Acid Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Yupeng Wu,Yu Zhou,Haiying Gao,Yajun Wang,Qingyu Cheng,Shikun Jian,Qi Ding,Wei Gu,Yanxue Yao,Jia Ma,Wenjuan Wu,Yuyun Li,Xuhui Tong,Xiaoyuan Song,Sai Ma

Molecular medicine reports 22:5282-5292 PubMed33174029

2020

MicroRNA‑145‑5p suppresses fascin to inhibit the invasion and migration of cervical carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shufang He,Guiyuan Yu,Ke Peng,Sisun Liu

Reproduction in domestic animals = Zuchthygiene 54:882-891 PubMed30974481

2019

MicroRNA-145 regulates immune cytokines via targeting FSCN1 in Staphylococcus aureus-induced mastitis in dairy cows.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Chen,Xin Xu,Taoling Tan,Daijie Chen,Huanjie Liang,Kaidi Sun,Mingxun Li,Huimin Zhang,Yongjiang Mao,Zhangping Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fascin antibody [FSCN1/417]

Immunocytochemistry - Anti-Fascin antibody [FSCN1/417] (AB220195)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (AB220195)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (AB220195)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [FSCN1/417] (AB220195)

Flow Cytometry - Anti-Fascin antibody [FSCN1/417] (AB220195)

Western blot - Anti-Fascin antibody [FSCN1/417] (AB220195)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

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