AB212841

Anti-Fascin antibody [SPM133] - BSA and Azide free

Name: Anti-Fascin antibody [SPM133] - BSA and Azide free (ab212841) | Abcam
Brand: Abcam Limited
SKU: AB212841
Price: 905 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Fascin antibody. Carrier free. Suitable for IHC-P, ICC, Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FSCN1.

View Alternative Names

FAN1, HSN, SNL, FSCN1, Fascin, 55 kDa actin-bundling protein, Singed-like protein, p55

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [SPM133] - BSA and Azide free (AB212841)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [SPM133] - BSA and Azide free (AB212841)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue labeling Fascin with ab212841 at 1 μg/mL.

Supplier Data

Immunocytochemistry - Anti-Fascin antibody [SPM133] - BSA and Azide free (AB212841)

Immunofluorescence Analysis of HeLa cells labeling Fascin with Fascin-1 with ab212841 (Green). The nuclear counterstain is Hoescht (Blue). Membrane is Phalloidin 488 (Red).

Flow Cytometry - Anti-Fascin antibody [SPM133] - BSA and Azide free (AB212841)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Fascin antibody [SPM133] - BSA and Azide free (AB212841)

Flow Cytometric Analysis of PFA-fixed K562 cells labeling Fascin-1 with ab212841 followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Supplier Data

Western blot - Anti-Fascin antibody [SPM133] - BSA and Azide free (AB212841)

All lanes:

Western blot - Anti-Fascin antibody [SPM133] - BSA and Azide free (ab212841) at 2 µg/mL

All lanes:

HeLa cell lysates

Predicted band size: 54 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SPM133

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, ICC, Flow Cyt, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FSCN1.

Q16658

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "(Primary incubation for 30 minutes at RT). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Actin-binding protein that contains 2 major actin binding sites (PubMed : 21685497, PubMed : 23184945). Organizes filamentous actin into parallel bundles (PubMed : 20393565, PubMed : 21685497, PubMed : 23184945). Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers (PubMed : 22155786). Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration (PubMed : 20393565, PubMed : 21685497, PubMed : 23184945). Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF (PubMed : 22155786).

See full target information FSCN1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cells 10: PubMed33477333

2021

SUMO-Activating Enzyme Subunit 1 (SAE1) Is a Promising Diagnostic Cancer Metabolism Biomarker of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiann Ruey Ong,Oluwaseun Adebayo Bamodu,Nguyen Viet Khang,Yen-Kuang Lin,Chi-Tai Yeh,Wei-Hwa Lee,Yih-Giun Cherng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fascin antibody [SPM133] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fascin antibody [SPM133] - BSA and Azide free (AB212841)

Immunocytochemistry - Anti-Fascin antibody [SPM133] - BSA and Azide free (AB212841)

Flow Cytometry - Anti-Fascin antibody [SPM133] - BSA and Azide free (AB212841)

Western blot - Anti-Fascin antibody [SPM133] - BSA and Azide free (AB212841)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise