AB51263

Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32]

Name: Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263) | Abcam
Brand: Abcam Limited
SKU: AB51263
Rating: 5 (8 reviews)

Lab Essentials
What is this?

(8 Reviews)

(98 Publications)

Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263) is a mouse monoclonal antibody detecting Fast Myosin Skeletal Heavy chain in IHC-P. Suitable for Rabbit.

- Over 70 publications
- Trusted since 2007

View Alternative Names

MYH1_HUMAN, MyHC-2x, MyHC-IIx/d, Myosin heavy chain, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d, Myosin-1, adult 1

2 Images

Lab

Immunocytochemistry - Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (AB51263)

Immunofluorescence staining of Fast Myosin Skeletal Heavy chain using ab51263 in ab277612, which were differentiated for 3 (left panel), 5 (middle panel) and 10 days (right panel) post induction.

The cells were fixed with 100% MeOH (5 min) and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab51263 at 5 µg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.

The antibody ab51263 gave comparable results using 4% formaldehyde fixation (10 min).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (AB51263)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (AB51263)

Immunohistochemistry analysis of Formalin-fixed, paraffin-embedded Rabbit tongue sections labeling Fast Myosin Skeletal Heavy chain with ab51263 at 20 μg/mL followed by Goat Anti-Mouse IgG (Fab specific)-FITC antibody.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MY-32

Isotype

IgG1

Carrier free

Reacts with

Rabbit

Applications

ICC, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-5 µg/mL", "ICC-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (ab51263) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Rabbit samples.

Trusted by the scientific community
Anti-Fast Myosin Skeletal Heavy chain [MY-32] (ab51263) was first used in a scientific publication in 2007 and has been cited over 70 times in peer-reviewed journals.

Reviewed by scientists
Anti-Fast Myosin Skeletal Heavy chain [MY-32] (ab51263) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Purification notes

Purified from Tissue culture supernatant.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information MYH1

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Publications (98)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6935 PubMed40721583

2025

3D bioprinting of plant and animal cell-based hybrid food.

Applications

Unspecified application

Species

Unspecified reactive species

Sushila Maharjan,Camila Yamashita,Cheng Pau Lee,Alejandro Villalobos Zepeda,Ana Karen Michel Farias,Andrea Duarte Rivera,Francisco Javier Aguilar Rojas,David Sebastian Rendon Ruiz,Armando Martinez Hernandez,David Hyram Hernandez Medina,Carlos Ezio Garciamendez-Mijares,Julia Japo,Ludivina Bermea Jimenez,Sonia Golombek,Alessandro Bentivogli,Michinao Hashimoto,Yu Shrike Zhang

Frontiers in physiology 16:1616693 PubMed40692696

2025

Targeting ERRs to counteract age-related muscle atrophy associated with physical inactivity: a pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto Bonanni,Angela Falvino,Antonio Matticari,Anna Maria Rinaldi,Giovanna D'Arcangelo,Pierangelo Cifelli,Riccardo Iundusi,Elena Gasbarra,Virginia Tancredi,Ida Cariati,Umberto Tarantino

Stem cell research & therapy 16:336 PubMed40597262

2025

VEGF-B-mediated myofiber types involved in high-fat diet-induced hyperglycemia through PKA-NFATs signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liu-Liu Shi,Yu-Ting Sun,Jia-Nan Sun,Jing Yue,Wei Chen,Kun Meng,Long Chen,Chang-Qing Hu,Rui Chen,Dong-Sheng Sun,Cheng-Bao Xu,Wei Yuan,Xin-Li Li,Dan Zhao,Yan Wu,Shi-Bing Xi,Xiao-Ying Zhao,Jun-Ming Tang

Poultry science 104:105419 PubMed40516297

2025

Developmental characteristics of skeletal muscle in goose during the embryonic and early post-hatching periods.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiqi Weng,Yi Liu,Huiying Wang,Guohong Chen,Daqian He,Qi Xu

Journal of cachexia, sarcopenia and muscle 16:e13752 PubMed40079370

2025

snRNA-Seq and Spatial Transcriptome Reveal Cell-Cell Crosstalk Mediated Metabolic Regulation in Porcine Skeletal Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Guo,Mengmeng Han,Junfei Xu,Wenyue Zhou,Hanjing Shi,Sisi Chen,Weijun Pang,Xing Zhang,Yehui Duan,Yulong Yin,Fengna Li

Animals : an open access journal from MDPI 15: PubMed39943074

2025

Effects of Free-Range Systems on Muscle Fiber Characteristics and Welfare Indicators in Geese.

Applications

Unspecified application

Species

Unspecified reactive species

Guoyao Wang,Jianzhou Chen,Yujiao Guo,Kaiqi Weng,Yu Zhang,Yang Zhang,Guohong Chen,Qi Xu,Yang Chen

BBA advances 7:100135 PubMed39839442

2025

Loss or inhibition of lysosomal acid lipase leads to cholesteryl ester accumulation without affecting muscle formation or mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Alena Akhmetshina,Laszlo Schooltink,Melina Amor,Katharina B Kuentzel,Silvia Rainer,Ananya Nandy,Hansjoerg Habisch,Tobias Madl,Elizabeth Rendina-Ruedy,Katharina Leithner,Nemanja Vujić,Dagmar Kratky

American journal of physiology. Gastrointestinal and liver physiology 328:G136-G144 PubMed39745592

2025

Local tissue response to a C-X-C motif chemokine ligand 12 therapy for fecal incontinence in a rabbit model.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah M Ruetten,Shannon S Lankford,Abolfazl S Abdolmaleki,Nicholas Edenhoffer,Gopal Badlani,James K Williams

Frontiers in physiology 15:1483594 PubMed39717825

2024

Entacapone alleviates muscle atrophy by modulating oxidative stress, proteolysis, and lipid aggregation in multiple mice models.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Zeng,Hanbing Xu,Mingzheng Wu,Xianlong Zhou,Pan Lei,Jiangtao Yu,Pinyi Wang,Haoli Ma,Yan Zhao

Biomolecules 14: PubMed39456222

2024

Modulation of Carnitine Palmitoyl Transferase 1b Expression and Activity in Muscle Pathophysiology in Osteoarthritis and Osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Greggi,Manuela Montanaro,Maria Giovanna Scioli,Martina Puzzuoli,Sonia Gino Grillo,Manuel Scimeca,Alessandro Mauriello,Augusto Orlandi,Elena Gasbarra,Riccardo Iundusi,Sabina Pucci,Umberto Tarantino

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32]

Immunocytochemistry - Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (AB51263)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain antibody [MY-32] (AB51263)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (98)

Product promise