Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (AB221149)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Fast Myosin Skeletal Heavy chain + MYH4 with ab221149 at 1/1000 dilution (0.505 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human skeletal muscle (PMID : 30274168, 25217814).

The section was incubated with ab221149 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (AB221149)

Fluorescence multiplex immunohistochemical analysis of the human skeletal muscle (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-delta Sarcoglycan (ab137101, green; Opal™690), anti-Fast Myosin Skeletal Heavy chain + MYH4 (ab221149, magenta; Opal™520) and anti-Slow Skeletal Myosin Heavy chain (ab234431, red; Opal™570) on human skeletal muscle. Panel B : anti-Fast Myosin Skeletal Heavy chain + MYH4 stained on fast type fibers. Panel C : anti-Slow Skeletal Myosin Heavy chain stained on slow type fibers. Panel D : anti-delta Sarcoglycan stained on membrane of skeletal muscle. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab137101 at 1/1000 (1.043 μg/ml), ab221149 at 1/1000 (0.505 μg/ml, and ab234431 at 1/4000 (0.255 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. DAPI (blue) was used as a nuclear counter stain. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (AB221149)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Fast Myosin Skeletal Heavy chain + MYH4 with ab221149 at 1/1000 dilution (0.505 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining on human liver (PMID : 30274168). The section was incubated with ab221149 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) .

• WB

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Western blot - Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (AB221149)

Blocking/diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] (ab221149) at 1/1000 dilution

All lanes:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

false

Exposure time: 37s