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AB228727

Anti-fast skeletal Myosin antibody

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(3 Publications)

Rabbit Polyclonal MYL1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MYL1.

View Alternative Names

MLC1/MLC3, MLC1F/MLC3F, Myosin light chain alkali 1/2, Myosin light chain A1/A2, MYL1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-fast skeletal Myosin antibody (AB228727)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-fast skeletal Myosin antibody (AB228727)

Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for fast skeletal Myosin (green) using ab228727 at 1/500 dilution in ICC/IF.

Nuclear counterstain : Hoechst 33342 (blue).

Western blot - Anti-fast skeletal Myosin antibody (AB228727)
  • WB

Supplier Data

Western blot - Anti-fast skeletal Myosin antibody (AB228727)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-fast skeletal Myosin antibody (ab228727) at 1/1000 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 21 kDa

false

Western blot - Anti-fast skeletal Myosin antibody (AB228727)
  • WB

Supplier Data

Western blot - Anti-fast skeletal Myosin antibody (AB228727)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-fast skeletal Myosin antibody (ab228727) at 1/10000 dilution

Lane 1:

Mouse heart lysate at 1 µg

Lane 2:

Mouse heart lysate at 5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 21 kDa

false

Western blot - Anti-fast skeletal Myosin antibody (AB228727)
  • WB

Supplier Data

Western blot - Anti-fast skeletal Myosin antibody (AB228727)

15% SDS-PAGE gel.

All lanes:

Western blot - Anti-fast skeletal Myosin antibody (ab228727) at 1/10000 dilution

All lanes:

Rat muscle lysate at 1 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 21 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MYL1. The exact immunogen used to generate this antibody is proprietary information.

P05976

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin heavy chain antibody [A4.1025] (AB37484)

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Primary Antibodies

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Primary Antibodies

AB47003

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fast skeletal Myosin also known as myosin heavy chain II is a motor protein involved in muscle contraction. It has an approximate mass of 220 kDa. Fast skeletal Myosin is expressed primarily in fast-twitch muscle fibers which are found in large quantities in skeletal muscles. These muscles are responsible for rapid and forceful movements. The protein interacts with actin filaments to facilitate contraction through the ATP-dependent sliding filament mechanism.
Biological function summary

Fast skeletal Myosin plays a critical role in the muscle contraction process. The protein forms a part of the myosin filament which together with actin composes the contractile apparatus within muscle fibers. It binds to ATP and hydrolyzes it releasing energy necessary for the movement of actin filaments. This interaction leads to the shortening of muscle fibers enabling muscle contraction and movement.

Pathways

Fast skeletal Myosin is an integral component of the muscle contraction and energy metabolism pathways. These pathways include the ATP generation and usage cycles essential for energy supply during contraction. Related proteins such as actin and tropomyosin interact with fast skeletal Myosin to regulate the contraction cycle. This regulation ensures proper muscle function coordination and responsiveness.

Fast skeletal Myosin is associated with conditions such as myopathies and muscular dystrophy. These disorders affect muscle fiber function leading to weakness and degeneration. In muscular dystrophy there is often a disruption in the expression or function of related proteins such as dystrophin which can exacerbate the dysfunction of fast skeletal Myosin. Understanding these associations can aid in the development of therapeutic interventions targeting these pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-regulatory myosin light chain required for proper formation and/or maintenance of myofibers, and thus appropriate muscle function.
See full target information MYL1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113081 PubMed40740498

2025

Desmosomal cadherin tension loss in pemphigus vulgaris mediated by the inhibition of active RhoA at cell-cell adhesions.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowei Jin,Jordan Rosenbohm,Eunju Kim,Bahareh Tajvidi Safa,Amir Ostadi Moghaddam,Kristina Seiffert-Sinha,Merced Leiker,Elijah Jones,Haiwei Zhai,Sindora R Baddam,Grayson Minnick,Yucheng Huo,James K Wahl,Fanben Meng,Changjin Huang,Jung Yul Lim,Daniel E Conway,Animesh A Sinha,Ruiguo Yang

NPJ Regenerative medicine 6:31 PubMed34078912

2021

Preclinical assessment on neuronal regeneration in the injury-related microenvironment of graphene-based scaffolds.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Qian,Xu Wang,Jialin Song,Wei Chen,Shuai Chen,Yi Jin,Yuanming Ouyang,Wei-En Yuan,Cunyi Fan

International journal of molecular sciences 21: PubMed33113794

2020

Increased Skeletal Muscle Fiber Cross-Sectional Area, Muscle Phenotype Shift, and Altered Insulin Signaling in Rat Hindlimb Muscles in a Prenatally Androgenized Rat Model for Polycystic Ovary Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Auryana DeChick,Rebecca Hetz,Jack Lee,Diana L Speelman
View all publications
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Product promise

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