Rabbit Polyclonal FASTK antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FASTK aa 350 to C-terminus.
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Publications
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- PLoS genetics 17:e10098732021The FASTK family proteins fine-tune mitochondrial RNA processing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkira Ohkubo,Lindsey Van Haute,Danielle L Rudler,Maike Stentenbach,Florian A Steiner,Oliver Rackham,Michal Minczuk,Aleksandra Filipovska,Jean-Claude MartinouPubMed 34748562
- PloS one 8:e723902013miR-106a-5p inhibits the proliferation and migration of astrocytoma cells and promotes apoptosis by targeting FASTK.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human FASTK aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q14296
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
| Dog | Predicted | Predicted |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog, Pig | Dilution info - | Notes - |
Target data
Function
Phosphorylates the splicing regulator TIA1, thereby promoting the inclusion of FAS exon 6, which leads to an mRNA encoding a pro-apoptotic form of the receptor. Isoform 4. Required for the biogenesis of some mitochondrial-encoded mRNAs, specifically stabilizes ND6 (NADH dehydrogenase complex subunit 6) mRNA, and regulates its levels.
Alternative names
Fas-activated serine/threonine kinase, FAST kinase, FASTK
Recommended products
Rabbit Polyclonal FASTK antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FASTK aa 350 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human FASTK aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q14296
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FASTK also known as Fas-activated serine/threonine kinase is an enzyme that functions primarily in the mitochondria of cells. This protein weighs approximately 65 kDa. FASTK is ubiquitously expressed in various tissues but it shows higher levels in heart and skeletal muscle. FASTK plays a role in RNA processing especially in regulating the stability of specific RNA molecules in mitochondria.
- Biological function summary
FASTK associates with a larger protein complex involved in mitochondrial RNA surveillance. This complex targets and stabilizes mitochondrial transcripts important for mitochondrial protein synthesis. Through its RNA-binding capability FASTK influences the production of ATP by maintaining proper mitochondrial function and energy metabolism.
- Pathways
FASTK operates within mitochondrial gene expression and oxidative phosphorylation pathways. It is associated with proteins like FASTKD2 and TFB2M which also participate in mitochondrial RNA processing and ribosome biogenesis. This interplay is essential for responding to cellular energy demands and maintaining cellular homeostasis.
- Associated diseases and disorders
Disruptions in FASTK activity relate to mitochondrial myopathy and neurodegenerative disorders such as Charcot-Marie-Tooth disease. Mutations in proteins like FASTKD2 and other mitochondrial RNA-binding proteins can influence these conditions highlighting the significance of FASTK in maintaining mitochondrial integrity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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6 product images
Western blot - Anti-FASTK antibody (ab97544)
All lanes: Western blot - Anti-FASTK antibody (ab97544) at 1/1000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Lane 3: HeLa whole cell lysate at 30 µg
Lane 4: HepG2 whole cell lysate at 30 µg
Lane 5: A375 whole cell lysate at 30 µg
Predicted band size: 61 kDa
Western blot - Anti-FASTK antibody (ab97544)
All lanes: Western blot - Anti-FASTK antibody (ab97544) at 1/1000 dilution
All lanes: PC-12 whole cell lysate at 30 µg
Predicted band size: 61 kDa
Western blot - Anti-FASTK antibody (ab97544)
All lanes: Western blot - Anti-FASTK antibody (ab97544) at 1/1000 dilution
All lanes: NIH-3T3 cell lysate at 30 µg
Predicted band size: 61 kDa
Immunocytochemistry/ Immunofluorescence - Anti-FASTK antibody (ab97544)
Immunofluorescent staining of FASTK in HeLa cells using ab97544 at a dilution of 1/1000. HeLa cells were fixed in ice-cold methanol for 5 minutes and the cells were counter-stained with Hoechst 33342.
Image from Zhi F et al., PLoS One. 2013;8(8):e72390. Fig 3.; doi: 10.1371/journal.pone.0072390 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Western blot - Anti-FASTK antibody (ab97544)
FASTK siRNA interference assay. Three siRNA sequences targeting different sites of human FASTK cDNA and a scrambled control siRNA (si-NC) were transfected into U251 cells using Lipofectamine 2000. Total protein or total RNA was isolated at 48 h or 24 h post-transfection. FASTK protein levels were determined by western blot analysis using ab97544 at a dilution of 1/500 (A). The secondary antibody was a rabbit IgG-HRP and the quantitive data was obtained with the Quantity One analysis program. FASTK mRNA levels were assessed by qRT-PCR (B).
All lanes: Western blot - Anti-FASTK antibody (ab97544)
Predicted band size: 61 kDa
Immunocytochemistry/ Immunofluorescence - Anti-FASTK antibody (ab97544)
Immunofluorescence analysis of FASTK in methanol fixed HeLa, using ab97544 at a 1/200 dilution.
Lower image is co-stained with Hoechst 33342.
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