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AB97544

Anti-FASTK antibody

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(2 Publications)

Rabbit Polyclonal FASTK antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FASTK aa 350 to C-terminus.

View Alternative Names

Fas-activated serine/threonine kinase, FAST kinase, FASTK

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-FASTK antibody (AB97544)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FASTK antibody (AB97544)

Immunofluorescence analysis of FASTK in methanol fixed HeLa, using ab97544 at a 1/200 dilution.
Lower image is co-stained with Hoechst 33342.

Immunocytochemistry/ Immunofluorescence - Anti-FASTK antibody (AB97544)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FASTK antibody (AB97544)

Immunofluorescent staining of FASTK in HeLa cells using ab97544 at a dilution of 1/1000. HeLa cells were fixed in ice-cold methanol for 5 minutes and the cells were counter-stained with Hoechst 33342.

Western blot - Anti-FASTK antibody (AB97544)
  • WB

PubMed

Western blot - Anti-FASTK antibody (AB97544)

FASTK siRNA interference assay. Three siRNA sequences targeting different sites of human FASTK cDNA and a scrambled control siRNA (si-NC) were transfected into U251 cells using Lipofectamine 2000. Total protein or total RNA was isolated at 48 h or 24 h post-transfection. FASTK protein levels were determined by western blot analysis using ab97544 at a dilution of 1/500 (A). The secondary antibody was a rabbit IgG-HRP and the quantitive data was obtained with the Quantity One analysis program. FASTK mRNA levels were assessed by qRT-PCR (B).

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Western blot - Anti-FASTK antibody (ab97544)

Predicted band size: 61 kDa

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Image from Zhi F et al., PLoS One. 2013;8(8):e72390. Fig 3.; doi: 10.1371/journal.pone.0072390 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Western blot - Anti-FASTK antibody (AB97544)
  • WB

Unknown

Western blot - Anti-FASTK antibody (AB97544)

All lanes:

Western blot - Anti-FASTK antibody (ab97544) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Lane 5:

A375 whole cell lysate at 30 µg

Predicted band size: 61 kDa

false

Western blot - Anti-FASTK antibody (AB97544)
  • WB

Unknown

Western blot - Anti-FASTK antibody (AB97544)

All lanes:

Western blot - Anti-FASTK antibody (ab97544) at 1/1000 dilution

All lanes:

PC-12 whole cell lysate at 30 µg

Predicted band size: 61 kDa

false

Western blot - Anti-FASTK antibody (AB97544)
  • WB

Unknown

Western blot - Anti-FASTK antibody (AB97544)

All lanes:

Western blot - Anti-FASTK antibody (ab97544) at 1/1000 dilution

All lanes:

NIH-3T3 cell lysate at 30 µg

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FASTK aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14296

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FASTK also known as Fas-activated serine/threonine kinase is an enzyme that functions primarily in the mitochondria of cells. This protein weighs approximately 65 kDa. FASTK is ubiquitously expressed in various tissues but it shows higher levels in heart and skeletal muscle. FASTK plays a role in RNA processing especially in regulating the stability of specific RNA molecules in mitochondria.
Biological function summary

FASTK associates with a larger protein complex involved in mitochondrial RNA surveillance. This complex targets and stabilizes mitochondrial transcripts important for mitochondrial protein synthesis. Through its RNA-binding capability FASTK influences the production of ATP by maintaining proper mitochondrial function and energy metabolism.

Pathways

FASTK operates within mitochondrial gene expression and oxidative phosphorylation pathways. It is associated with proteins like FASTKD2 and TFB2M which also participate in mitochondrial RNA processing and ribosome biogenesis. This interplay is essential for responding to cellular energy demands and maintaining cellular homeostasis.

Disruptions in FASTK activity relate to mitochondrial myopathy and neurodegenerative disorders such as Charcot-Marie-Tooth disease. Mutations in proteins like FASTKD2 and other mitochondrial RNA-binding proteins can influence these conditions highlighting the significance of FASTK in maintaining mitochondrial integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phosphorylates the splicing regulator TIA1, thereby promoting the inclusion of FAS exon 6, which leads to an mRNA encoding a pro-apoptotic form of the receptor.. Isoform 4. Required for the biogenesis of some mitochondrial-encoded mRNAs, specifically stabilizes ND6 (NADH dehydrogenase complex subunit 6) mRNA, and regulates its levels.
See full target information FASTK
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 17:e1009873 PubMed34748562

2021

The FASTK family proteins fine-tune mitochondrial RNA processing.

Applications

Unspecified application

Species

Unspecified reactive species

Akira Ohkubo,Lindsey Van Haute,Danielle L Rudler,Maike Stentenbach,Florian A Steiner,Oliver Rackham,Michal Minczuk,Aleksandra Filipovska,Jean-Claude Martinou

PloS one 8:e72390 PubMed24013584

2013

miR-106a-5p inhibits the proliferation and migration of astrocytoma cells and promotes apoptosis by targeting FASTK.

Applications

WB

Species

Unspecified reactive species

Feng Zhi,Guangxin Zhou,Naiyuan Shao,Xiwei Xia,Yimin Shi,Qiang Wang,Yi Zhang,Rong Wang,Lian Xue,Suinuan Wang,Sujia Wu,Ya Peng,Yilin Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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