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AB235113

Anti-FASTKD2 antibody

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(1 Publication)

Rabbit Polyclonal FASTKD2 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FASTKD2 aa 100-250.

View Alternative Names

KIAA0971, FASTKD2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FASTKD2 antibody (AB235113)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FASTKD2 antibody (AB235113)

Paraffin-embedded human spleen tissue stained for FASTKD2 using ab235113 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FASTKD2 antibody (AB235113)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FASTKD2 antibody (AB235113)

Paraffin-embedded human heart tissue stained for FASTKD2 using ab235113 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.

Western blot - Anti-FASTKD2 antibody (AB235113)
  • WB

Supplier Data

Western blot - Anti-FASTKD2 antibody (AB235113)

All lanes:

Western blot - Anti-FASTKD2 antibody (ab235113) at 1/500 dilution

Lane 1:

Rat heart lysate

Lane 2:

Mouse heart lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 81 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human FASTKD2 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q9NYY8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/300", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FASTKD2 or FAST kinase domains 2 is a protein with a mass of approximately 83 kDa. It primarily localizes in the mitochondria and plays an important role in RNA processing events there. FASTKD2 is ubiquitously expressed with higher levels observed in tissues with high-energy demands such as the brain heart and skeletal muscle. The protein is recognized for its kinase domains which categorically engage in the modulation of RNA.
Biological function summary

FASTKD2 influences mitochondrial RNA metabolism and helps in the processing of various RNA species. It is a part of the FASTK protein family which consists of several members known for regulating RNA function. FASTKD2 does not act alone; it often interacts with other components of the mitochondrial ribonucleoprotein complex. This interaction is fundamental for ensuring that mitochondria maintain energetic efficiency through effective RNA management.

Pathways

Mitochondrial RNA processing and homeostasis tightly involve FASTKD2. It participates in the mitochondrial translation pathway and the assembly of mitochondrial ribosomes. FASTKD2 has associations with other proteins such as FASTKD3 which shares functional similarities and often cooperates in RNA-related activities within the cell. Such interactions highlight its integrated role in maintaining mitochondrial function.

Defects in FASTKD2 can lead to mitochondrial encephalomyopathy and other mitochondrial dysfunction syndromes. The protein's impairment affects neurological development often contributing to severe complications. FASTKD2 has observed linkage with FASTKD5 in related disorders reflecting their interconnected roles in helping maintain mitochondrial genomic integrity. Understanding these pathways may offer insights into potential therapies for such mitochondrial-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in assembly of the mitochondrial large ribosomal subunit (PubMed : 25683715). As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mitochondrial ribosomal RNA (16S mt-rRNA), controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation (PubMed : 25683715, PubMed : 26370583, PubMed : 27667664). May play a role in mitochondrial apoptosis.
See full target information FASTKD2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 14: PubMed38254638

2024

GDF-15 Inhibits ADP-Induced Human Platelet Aggregation through the GFRAL/RET Signaling Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Baikang Xie,Wenjing Tang,Shuang Wen,Fen Chen,Chao Yang,Min Wang,Yong Yang,Wei Liang
View all publications
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Product promise

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