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AB130076

Anti-FAT4 antibody

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(8 Publications)

Rabbit Polyclonal FAT4 antibody. Suitable for ICC/IF, WB and reacts with Human, Recombinant full length protein samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human FAT4 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CDHF14, FATJ, Nbla00548, FAT4, Protocadherin Fat 4, hFat4, Cadherin family member 14, FAT tumor suppressor homolog 4, Fat-like cadherin protein FAT-J

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-FAT4 antibody (AB130076)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FAT4 antibody (AB130076)

Immunocytochemistry analysis of HepG2 cells labeling FAT tumor suppressor homolog 4 with ab130076 at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% TritonX-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with ab130076 at 1/200 dilution in 2% negative goat serum overnight at 4℃.

Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1000 dilution.

PBS instead of the primary antibody was used as the secondary antibody only control.

Nuclear DNA was labeled in blue with DAPI.

Western blot - Anti-FAT4 antibody (AB130076)
  • WB

Unknown

Western blot - Anti-FAT4 antibody (AB130076)

actual MW : 48kDa

All lanes:

Western blot - Anti-FAT4 antibody (ab130076) at 1/500 dilution

All lanes:

recombinant FAT4 protein

Predicted band size: 543 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human FAT4 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q6V0I7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.05% Sodium azide Constituents: 74% Tris buffered saline, 25% Glycerol (glycerin, glycerine), 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FAT atypical cadherin 4 often referred to as FAT4 is a large transmembrane protein with a mass of approximately 560 kDa. It belongs to the cadherin superfamily and contains multiple cadherin repeats EGF-like domains and one laminin G-like domain. FAT4 is expressed widely across tissues with higher expression observed in the kidney liver and cardiovascular system indicating its involvement in diverse physiological processes. This protein plays a role in maintaining cell adhesion and communication.
Biological function summary

FAT4 plays an important role in the regulation of planar cell polarity and tissue organization. It exists within the context of cellular signaling complexes where it functions as a signal transducer that affects cell morphology and motility. Through its interactions with other cadherin family members FAT4 helps coordinate cellular alignment and directional growth. These functions contribute to the proper formation of organ structure and maintain tissue architecture.

Pathways

FAT4 is heavily involved in the Hippo signaling pathway which regulates cell proliferation apoptosis and organ size. It interacts with other proteins like dystrophin and LATS1/2 kinases within this pathway affecting cellular behaviors that are important for development and homeostasis. FAT4 has also been linked to the Wnt signaling pathway where it modulates beta-catenin interactions further linking it to cellular and developmental processes.

FAT4 has connections to Van Maldergem syndrome and certain cancers. Alterations in FAT4 expression or function can lead to defects in tissue formation and cancer progression due to its role in controlling cell growth and polarity. In particular FAT4 mutations have correlated with breast cancer where it interacts with proteins such as YAP and TAZ indicating its potential as a therapeutic target. Understanding FAT4's role in these diseases is key to developing targeted treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cadherins are calcium-dependent cell adhesion proteins. FAT4 plays a role in the maintenance of planar cell polarity as well as in inhibition of YAP1-mediated neuroprogenitor cell proliferation and differentiation (By similarity).
See full target information FAT4
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Breast cancer research : BCR 27:163 PubMed41013770

2025

An enteropathogenic microbial toxin modulates the breast cancer epigenome resulting in concurrent silencing of tumor suppressor genes.

Applications

Unspecified application

Species

Unspecified reactive species

Deepak Verma,Deeptashree Nandi,Sheetal Parida,Archisha Saxena,Dipali Sharma

Nature communications 14:1980 PubMed37031213

2023

Endothelial FAT1 inhibits angiogenesis by controlling YAP/TAZ protein degradation via E3 ligase MIB2.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Li,Jingchen Shao,Young-June Jin,Haruya Kawase,Yu Ting Ong,Kerstin Troidl,Qi Quan,Lei Wang,Remy Bonnavion,Astrid Wietelmann,Francoise Helmbacher,Michael Potente,Johannes Graumann,Nina Wettschureck,Stefan Offermanns

Bioscience reports 40: PubMed33063118

2020

MiR-106b-5p regulates the migration and invasion of colorectal cancer cells by targeting FAT4.

Applications

Unspecified application

Species

Unspecified reactive species

Min Pan,Qiuqiu Chen,Yusong Lu,Feifei Wei,Chunqiao Chen,Guiyan Tang,Hui Huang

BMC cancer 20:374 PubMed32366234

2020

FAT4 silencing promotes epithelial-to-mesenchymal transition and invasion via regulation of YAP and β-catenin activity in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shika Hanif Malgundkar,Ikram Burney,Mansour Al Moundhri,Moza Al Kalbani,Ritu Lakhtakia,Aikou Okamoto,Yahya Tamimi

Translational cancer research 9:309-322 PubMed35117185

2020

Up-regulation of FAT4 enhances the chemosensitivity of colorectal cancer cells treated by 5-FU.

Applications

Unspecified application

Species

Unspecified reactive species

Qianyuan Li,Xiukou Zhou,Zhengyu Fang,Zhiyun Pan,Huamiao Zhou

American journal of translational research 11:2784-2800 PubMed31217854

2019

FAT4-USP51 complex regulates the proliferation and invasion of endometrial cancer via Hippo pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxia Che,Fangfang Jian,Nan Jia,Ya Zheng,Yahui Jiang,Weiwei Feng

Cancer gene therapy 26:374-387 PubMed30546116

2018

Down-regulated long non-coding RNA RNAZFHX4-AS1 suppresses invasion and migration of breast cancer cells via FAT4-dependent Hippo signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shao-Ying Li,Hong Wang,Hui-Fang Mai,Guo-Feng Li,Shao-Jun Chen,Gui-Sen Li,Bi-Chan Liang

Cancer biology & therapy 17:36-47 PubMed26575609

2015

Fat4 suppression induces Yap translocation accounting for the promoted proliferation and migration of gastric cancer cells.

Applications

WB

Species

Human

Liangang Ma,Jianxin Cui,Hongqing Xi,Shibo Bian,Bo Wei,Lin Chen
View all publications
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Product promise

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