Anti-Fatty Acid Synthase antibody (ab22759) is a rabbit polyclonal antibody that is used to detect Fatty Acid Synthase in Western Blot. Suitable for Human, Mouse, Rat samples.
- Specificity confirmed with Fatty Acid Synthase knockout cell line validation
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- The American journal of pathology 194:253-2632023Nonmetastatic Axillary Lymph Nodes Have Distinct Morphology and Immunophenotype in Obese Patients with Breast Cancer at Risk for Metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingyuan Song,Kristen E Muller,Liesbeth M Hondelink,Roberta M diFlorio-Alexander,Margaret R Karagas,Saeed HassanpourPubMed 38029922
- Journal of neuroinflammation 20:2642023Glyceryl triacetate promotes blood-brain barrier recovery after ischemic stroke through lipogenesis-mediated IL-33 in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaidong Wei,Luming Zhen,Shiquan Wang,Liufei Yang,Shuyue Zhang,Yuanyuan Zhang,Pengyu Jia,Tianyue Wang,Kui Wang,Yan Zhang,Lei Ma,Jianrui Lv,Pengbo ZhangPubMed 37968698
- Nutrition & metabolism 20:442023Differential effects of plant-based flours on metabolic homeostasis and the gut microbiota in high-fat fed rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaylor M Martinez,Hallie R Wachsmuth,Rachel K Meyer,Savanna N Weninger,Adelina I Lane,Archana Kangath,Gabriele Schiro,Daniel Laubitz,Jennifer H Stern,Frank A DucaPubMed 37858106
- Journal of dairy science 106:9868-98782023Effects of choline deficiency and supplementation on lipid droplet accumulation in bovine primary liver cells in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenyan Lu,Jingna Yang,Mingyue Hu,Kai Zhong,Yueying Wang,Yanbin Yang,Juan J Loor,Guoyu Yang,Liqiang HanPubMed 37678795
- Scientific reports 13:90442023Overexpression of fatty acid synthase attenuates bleomycin induced lung fibrosis by restoring mitochondrial dysfunction in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyesun Shin,Shinhee Park,Jisu Hong,Ae-Rin Baek,Junehyuk Lee,Do-Jin Kim,An-Soo Jang,Su Sie Chin,Sung Hwan Jeong,Sung-Woo ParkPubMed 37270622
- British journal of cancer 128:1991-19992023MBTPS2 acts as a regulator of lipogenesis and cholesterol synthesis through SREBP signalling in prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmy J Tibbo,Andrew Hartley,Richa Vasan,Robin Shaw,Laura Galbraith,Ernest Mui,Hing Y Leung,Imran AhmadPubMed 36991255
- Nature communications 14:13622023De novo lipogenesis fuels adipocyte autophagosome and lysosome membrane dynamics.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeslie A Rowland,Adilson Guilherme,Felipe Henriques,Chloe DiMarzio,Sean Munroe,Nicole Wetoska,Mark Kelly,Keith Reddig,Gregory Hendricks,Meixia Pan,Xianlin Han,Olga R Ilkayeva,Christopher B Newgard,Michael P CzechPubMed 36914626
- Animal nutrition (Zhongguo xu mu shou yi xue hui) 13:126-1362023Sodium acetate promotes fat synthesis by suppressing TATA element modulatory factor 1 in bovine mammary epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChaochao Luo,Nan Li,Qingzhu Wang,Chunjiang LiPubMed 37123620
- Respiratory research 23:3382022D-Limonene inhibits the occurrence and progression of LUAD through suppressing lipid droplet accumulation induced by PM exposure in vivo and in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTengteng Zhu,Yangyang Li,Tienan Feng,Yuqing Yang,Kai Zhang,Jing Gao,Xiaowei Quan,Ying Qian,Herbert Yu,Biyun QianPubMed 36496421
- Frontiers in pharmacology 13:9155922022Bailing capsule () ameliorates renal triglyceride accumulation through the PPARα pathway in diabetic rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Zhang,Xinhua Xiao,Ming Li,Miao Yu,Fan PingPubMed 36091833
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| ICC | WB | IHC (PFA fixed) | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
| Mouse | Expected | Tested | Expected |
| Rat | Expected | Expected | Expected |
| Chicken | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes - |
Species Rat | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow | Dilution info - | Notes - |
IHC (PFA fixed)
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 2 µg/mL | Notes - |
Species Human | Dilution info 2 µg/mL | Notes - |
Species Mouse | Dilution info 2 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.
Alternative names
Fatty acid synthase, Type I Fatty Acid Synthase
Recommended products
Anti-Fatty Acid Synthase antibody (ab22759) is a rabbit polyclonal antibody that is used to detect Fatty Acid Synthase in Western Blot. Suitable for Human, Mouse, Rat samples.
- Specificity confirmed with Fatty Acid Synthase knockout cell line validation
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab128870.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Fatty Acid Synthase antibody (ab22759) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.
What is the molecular weight of Fatty Acid Synthase?
Anti-Fatty Acid Synthase (ab22759) specifically detects a band for Fatty Acid Synthase (UniProt: P49327) at a molecular weight of 273kDa.
Trusted by the scientific community
Anti-Fatty Acid Synthase (ab22759) was first used in a scientific publication in 2006 and has been cited over 110 times in peer-reviewed journals.
Reviewed by scientists
Anti-Fatty Acid Synthase (ab22759) has over 10 independent reviews from customers.
Specificity confirmed
The specificity of Anti-Fatty Acid Synthase antibody (ab22759) has been confirmed by Western blot testing in Fasn Knockout HAP1 cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Western blot - Anti-Fatty Acid Synthase antibody (ab22759)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 μg)
Lane 3: A549 cell lysate (20 μg)
Lane 4: Hu liver tissue lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab22759 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab22759 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab22759 and ab18058 (loading control to Vinculin) were diluted at 1 μg/ml and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Fatty Acid Synthase antibody (ab22759)
Predicted band size: 273 kDa
Immunocytochemistry - Anti-Fatty Acid Synthase antibody (ab22759)
ab22759 staining Fatty Acid Synthase in HeLa cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab22759 at 5 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Western blot - Anti-Fatty Acid Synthase antibody (ab22759)
All lanes: Western blot - Anti-Fatty Acid Synthase antibody (ab22759) at 1 µg/mL
Lane 1: 3T3-L1 nuclear extract lysate (ab14632) at 20 µg
Lane 2: Brain (Mouse) Tissue Lysate (ab27253) at 20 µg
Lane 3: Liver (Mouse) Tissue Lysate (ab7935) at 20 µg
Lane 4: 3T3-L1 nuclear extract lysate (ab14632) at 20 µg with Mouse Fatty Acid Synthase peptide (ab25719)
Lane 5: Brain (Mouse) Tissue Lysate (ab27253) at 20 µg with Mouse Fatty Acid Synthase peptide (ab25719)
Lane 6: Liver (Mouse) Tissue Lysate (ab7935) at 20 µg with Mouse Fatty Acid Synthase peptide (ab25719)
SecondaryAll lanes: Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 273 kDa
Observed band size: 100 kDa, 273 kDa, 35 kDa, 50 kDa
This image is courtesy of Sophie Pezet, King's College London, United KingdomImmunohistochemistry (PFA fixed) - Anti-Fatty Acid Synthase antibody (ab22759)
Immunofluorescent staining for Fatty Acid Synthase in the rat striatum using Rabbit polyclonal to Fatty Acid Synthase (ab22759). Abundant staining was observed in the Striatum with lower levels of staining observed in the Corpus callosum. This is a montage of three pictures aquired using a X10 objective. ab22759 was used at 1/200 (2μg/ml) incubated overnight at room temperature.
Secondary antibody used was anti-rabbit Alexa Fluor® 488 at 1/1000 incubated for 2 hours at room temperature. Rat brain tissue was perfusion fixed with 4% PFA followed by overnight post-fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. 30μm coronal sections were cut on a cyrostat and immunohistochemistry performed by the 'free floating' technique.
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