Rabbit Recombinant Monoclonal Fatty Acid Synthase antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 21 publications.
Images
![Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (AB128856), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7465-ab128856--western-blot-img177624.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (AB128856)")
![Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] (AB128856), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7465-ab128856--flow-cytometry-intracellular-img168252.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] (AB128856)")
![Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (AB128856), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7465-ab128856--immunocytochemistry-immunofluorescence-img168615.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (AB128856)")
![Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (AB128856), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7465-ab128856--western-blot-img2869.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (AB128856)")
Publications
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- Respiratory research 24:2532023Silencing of CD147 inhibits cell proliferation, migration, invasion, lipid metabolism dysregulation and promotes apoptosis in lung adenocarcinoma via blocking the Rap1 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNing Zhang,Zhouzhong Liu,Xuwang Lai,Shubin Liu,Yuli WangPubMed 37880644
- Food & nutrition research 67:2023Antidiabetic and antihyperlipidemic activities of . extract and the regulation of Akt phosphorylation, gluconeogenesis, and peroxisome proliferator-activated receptor α in streptozotocin-induced diabetic mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShin-Ming Huang,Cheng-Hsiu Lin,Wen-Fang Chang,Chun-Ching ShihPubMed 37850072
- Life science alliance 6:2022Hepatic DKK1-driven steatosis is CD36 dependent.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhen Yang,Xinping Huang,Jiaye Zhang,Kai You,Yue Xiong,Ji Fang,Anteneh Getachew,Ziqi Cheng,Xiaorui Yu,Yan Wang,Feima Wu,Ning Wang,Shufen Feng,Xianhua Lin,Fan Yang,Yan Chen,Hongcheng Wei,Yin-Xiong LiPubMed 36410795
- Cell reports 38:1104602022Proteome-centric cross-omics characterization and integrated network analyses of triple-negative breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian-Qi Gong,Yi-Zhou Jiang,Chen Shao,Wen-Ting Peng,Ming-Wei Liu,Da-Qiang Li,Ben-Yu Zhang,Peng Du,Yin Huang,Fei-Fei Li,Mu-Yun Li,Zhao-Lian Han,Xi Jin,Ding Ma,Yi Xiao,Peng-Yuan Yang,Jun Qin,Zhi-Ming Shao,Weimin ZhuPubMed 35235781
- Frontiers in cell and developmental biology 9:7792572022Keratin 17 Is Required for Lipid Metabolism in Keratinocytes and Benefits Epidermal Permeability Barrier Homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBingyu Pang,Zhenlai Zhu,Chunying Xiao,Yixin Luo,Hui Fang,Yaxing Bai,Zhongbin Sun,Jingyi Ma,Erle Dang,Gang WangPubMed 35096815
- Frontiers in medicine 8:7675842021Integrated Analyses Identify Key Molecules and Reveal the Potential Mechanism of miR-182-5p/FOXO1 Axis in Alcoholic Liver Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhihua Zuo,Yiqin Li,Chuyi Zeng,Yuge Xi,Hualin Tao,Yongcan GuoPubMed 34950682
- PLoS biology 19:e30010852021The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatrick A Carroll,Brian W Freie,Pei Feng Cheng,Sivakanthan Kasinathan,Haiwei Gu,Theresa Hedrich,James A Dowdle,Vivek Venkataramani,Vijay Ramani,Xiaoying Wu,Daniel Raftery,Jay Shendure,Donald E Ayer,Charles H Muller,Robert N EisenmanPubMed 34669700
- International journal of molecular sciences 22:2021Mouse Liver Compensates Loss of Sgpl1 by Secretion of Sphingolipids into Blood and Bile.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Katharina Spohner,Katja Jakobi,Sandra Trautmann,Dominique Thomas,Fabian Schumacher,Burkhard Kleuser,Dieter Lütjohann,Khadija El-Hindi,Sabine Grösch,Josef Pfeilschifter,Julie D Saba,Dagmar Meyer Zu HeringdorfPubMed 34638955
- Nature 590:480-4852021Creatine kinase B controls futile creatine cycling in thermogenic fat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJanane F Rahbani,Anna Roesler,Mohammed F Hussain,Bozena Samborska,Christien B Dykstra,Linus Tsai,Mark P Jedrychowski,Laurent Vergnes,Karen Reue,Bruce M Spiegelman,Lawrence KazakPubMed 33597756
- The Journal of biological chemistry 295:3891-39052020Vitamin D receptor targets hepatocyte nuclear factor 4α and mediates protective effects of vitamin D in nonalcoholic fatty liver disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong Zhang et. al.PubMed 32051143
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested | Tested |
| Mouse | Not recommended | Tested | Not recommended | Expected | Expected |
| Rat | Not recommended | Tested | Not recommended | Expected | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes For unpurified version, use at 1/250 - 1/500 dilution. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain. (Microbial infection) Fatty acid synthetase activity is required for SARS coronavirus-2/SARS-CoV-2 replication.
Alternative names
FAS, FASN, Fatty acid synthase, Type I fatty acid synthase
Recommended products
Rabbit Recombinant Monoclonal Fatty Acid Synthase antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 21 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR7465
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.58 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7465-ab128856--western-blot-img177624.jpg "Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)")
Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
All lanes: Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856) at 1/5000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg
Lane 5: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Lane 6: Mouse brain lysate at 20 µg
Lane 7: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 8: Rat brain lysate at 20 µg
Lane 9: L6 (Rat skeletal muscle myoblast) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 273 kDa
Observed band size: 237 kDa, 273 kDa
![Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7465-ab128856--flow-cytometry-intracellular-img168252.jpg "Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)")
Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labelling Fatty Acid Synthase with Purified ab128856 at 1:50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7465-ab128856--immunocytochemistry-immunofluorescence-img168615.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)")
Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Fatty Acid Synthase with Purified ab128856 at 1:50 dilution (4.5 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with . Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7465-ab128856--western-blot-img2869.jpg "Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)")
Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 μg)
Lane 3: A549 cell lysate (20 μg)
Lane 4: Hu liver tissue lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab128856 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.ab128856 was shown to specifically react with Fatty Acid Synthase in wild-type HAP1 cells. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab128856 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (ab128856)
Predicted band size: 273 kDa
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