Rabbit Recombinant Monoclonal Fatty Acid Synthase antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Images
![Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (AB227083), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7465-bsa-and-azide-free-ab227083--western-blot-img177624.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (AB227083)")
![Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (AB227083), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7465-bsa-and-azide-free-ab227083--flow-cytometry-intracellular-img168252.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (AB227083)")
![Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (AB227083), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7465-bsa-and-azide-free-ab227083--immunocytochemistry-immunofluorescence-img168615.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (AB227083)")
![Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (AB227083), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7465-bsa-and-azide-free-ab227083--western-blot-img2869.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (AB227083)")
Publications
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- PloS one 12:e01787482017Caveolin1 protects against diet induced hepatic lipid accumulation in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng Li et. al.PubMed 28570612
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested | Tested |
| Mouse | Not recommended | Tested | Not recommended | Expected | Expected |
| Rat | Not recommended | Tested | Not recommended | Expected | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 | Notes - |
Species Mouse | Dilution info 1/5000 | Notes - |
Species Rat | Dilution info 1/5000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
3 products for Alternative Version
Target data
Function
Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain. (Microbial infection) Fatty acid synthetase activity is required for SARS coronavirus-2/SARS-CoV-2 replication.
Alternative names
FAS, FASN, Fatty acid synthase, Type I fatty acid synthase
Recommended products
Rabbit Recombinant Monoclonal Fatty Acid Synthase antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR7465
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.58 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab227083 is the carrier-free version of Anti-Fatty Acid Synthase antibody [EPR7465] ab128856.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7465-bsa-and-azide-free-ab227083--western-blot-img177624.jpg "Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)")
Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
This data was developed using Anti-Fatty Acid Synthase antibody [EPR7465] ab128856, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (Anti-Fatty Acid Synthase antibody [EPR7465] ab128856) at 1/5000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 3: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg
Lane 5: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Lane 6: Mouse brain lysate at 20 µg
Lane 7: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 8: Rat brain lysate at 20 µg
Lane 9: L6 (Rat skeletal muscle myoblast) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 273 kDa
Observed band size: 237 kDa, 273 kDa
![Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7465-bsa-and-azide-free-ab227083--flow-cytometry-intracellular-img168252.jpg "Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)")
Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
This data was developed using Anti-Fatty Acid Synthase antibody [EPR7465] ab128856, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labelling Fatty Acid Synthase with Purified Anti-Fatty Acid Synthase antibody [EPR7465] ab128856 at 1:50 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).![Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7465-bsa-and-azide-free-ab227083--immunocytochemistry-immunofluorescence-img168615.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)")
Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
This data was developed using Anti-Fatty Acid Synthase antibody [EPR7465] ab128856, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Fatty Acid Synthase with Purified Anti-Fatty Acid Synthase antibody [EPR7465] ab128856 at 1:50 dilution (4.5 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with . Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.![Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7465-bsa-and-azide-free-ab227083--western-blot-img2869.jpg "Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)")
Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] - BSA and Azide free (ab227083)
This WB data was generated using the same anti-Fatty Acid Synthase antibody clone, EPR7465, in a different buffer formulation (cat# Anti-Fatty Acid Synthase antibody [EPR7465] ab128856).
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 μg)
Lane 3: A549 cell lysate (20 μg)
Lane 4: Hu liver tissue lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Fatty Acid Synthase antibody [EPR7465] ab128856 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.Anti-Fatty Acid Synthase antibody [EPR7465] ab128856 was shown to specifically react with Fatty Acid Synthase when Fatty Acid Synthase knockout samples were used. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. Anti-Fatty Acid Synthase antibody [EPR7465] ab128856 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
All lanes: Western blot - Anti-Fatty Acid Synthase antibody [EPR7465] (Anti-Fatty Acid Synthase antibody [EPR7465] ab128856)
Predicted band size: 273 kDa
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