Anti-Fatty Acid Synthase antibody [EPR7466]

10 product images

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  Immunoprecipitation - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

  Purified ab128870 at 1:30 dilution (2μg) immunoprecipitating Fatty Acid Synthase in HEK-293 whole cell lysate.
  Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10 μg
  Lane 2 (+): ab128870 + HEK-293 whole cell lysate.
  Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab128870 in HEK-293 whole cell lysate.
  VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) (1:10,000 dilution) was used for Western blotting.
  Blocking Buffer and concentration: 5% NFDM/TBST.
  Diluting buffer and concentration: 5% NFDM/TBST.
  Observed band size: kDa

  All lanes: Immunoprecipitation - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

  Predicted band size: 273 kDa

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  Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

  All lanes: Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870) at 1/1000 dilution

  Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 2: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 3: Mouse liver lysate at 20 µg

  Lane 4: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

  Lane 5: Mouse brain lysate at 20 µg

  Lane 6: L6 (Rat skeletal muscle myoblast) whole cell lysate at 20 µg

  Lane 7: Rat brain lysate at 20 µg

  Lane 8: Rat liver lysate at 20 µg

  Lane 9: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Predicted band size: 273 kDa

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  Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

  Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labelling Fatty Acid Synthase with Purified ab128870 at 1:50 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling Fatty Acid Synthase with Purified ab128870 at 1:450 dilution (1.09 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling Fatty Acid Synthase with Purified ab128870 at 1:450 dilution (1.09 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

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  Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

  Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Fatty Acid Synthase with Purified ab128870 at 1:50 dilution (10 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Fatty Acid Synthase with Purified ab128870 at 1:450 dilution (1.09 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

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  Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

  Lane 1: Wild-type HAP1 cell lysate (20 μg)
  Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 μg)
  Lane 3: A549 cell lysate (20 μg)
  Lane 4: Hu liver tissue lysate (20 μg)
  Lanes 1 - 4: Merged signal (red and green). Green - ab128870 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.
  

  ab128870 was shown to react with Fatty Acid Synthase in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. ab128870 and ab18058 (loading control to Vinculin) were both diluted at 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  All lanes: Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

  Predicted band size: 273 kDa

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  Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling with ab128870 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cells is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
  

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at 1/1000 dilution.

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  Image collected and cropped by CiteAb under a CC-BY license from the publication

  Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] (ab128870)

  Fatty Acid Synthase western blot using anti-Fatty Acid Synthase antibody [EPR7466] ab128870. Publication image and figure legend from Avraham, O., Deng, P. Y., et al., 2020, Nat Commun, PubMed 32994417.

  
  

  ab128870 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab128870 please see the product overview.

  SGC upregulate genes involved in lipid metabolism in response to nerve injury.a Pathway analysis (KEGG 2016) of differentially upregulated genes in the SGC cluster. n = 2 biologically independent experiments. (FDR < 0.05, Log2Fold change >2). b t-SNE overlay for expression of Fasn gene. c DRG sections from control and FasncKO mice, immunostained for FASN (green) and the neuronal marker TUJ1 (magenta). Scale bar: 50 µm. d Nerve sections from control and FasncKO mice, immunostained for FASN (green) and the neuronal marker TUJ1 (magenta). Scale bar: 50 µm. e Western blot analysis and quantification of FASN protein expression in DRG from control and FasncKO mice with and without injury. Quantification of FASN expression normalized to GAPDH expression. ns-non significant. Source data are provided as a Source Data file. f Western blot analysis and quantification of FASN protein expression in Sciatic nerve from control and FasncKO mice with and without injury. Quantification of FASN expression normalized to GAPDH expression. ns-non significant. Source data are provided as a Source Data file. n = 3 biologically independent animals in (e, f). Data are presented as mean values ± SEM in (e, f). One way ANOVA followed by Dunnett’s multiple comparisons test in (e, f).