Rabbit Recombinant Monoclonal Fatty Acid Synthase antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (AB221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--immunoprecipitation-img169715.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (AB221934)")
![Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (AB221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--western-blot-img165657.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (AB221934)")
![Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (AB221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--flow-cytometry-intracellular-img169261.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (AB221934)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (AB221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img180914.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (AB221934)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (AB221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img171868.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (AB221934)")
Publications
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- The Journal of clinical investigation 134:2024Inhibiting the NADase CD38 improves cytomegalovirus-specific CD8+ T cell functionality and metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNils Mülling,Felix M Behr,Graham A Heieis,Kristina Boss,Suzanne van Duikeren,Floortje J van Haften,Iris N Pardieck,Esmé Ti van der Gracht,Ward Vleeshouwers,Tetje C van der Sluis,J Fréderique de Graaf,Dominique Mb Veerkamp,Kees Lmc Franken,Xin Lei,Lukas van de Sand,Sjoerd H van der Burg,Marij Jp Welters,Sebastiaan Heidt,Wesley Huisman,Simon P Jochems,Martin Giera,Oliver Witzke,Aiko Pj de Vries,Andreas Kribben,Bart Everts,Benjamin Wilde,Ramon ArensPubMed 38954588
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected | Expected |
| Rat | Expected | Expected | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes This antibody does not work well in liver tissue in WB application. We suggest ab128856 as an alternative. |
Species Rat | Dilution info - | Notes This antibody does not work well in liver tissue in WB application. We suggest ab128856 as an alternative. |
Species Human | Dilution info - | Notes This antibody does not work well in liver tissue in WB application. We suggest ab128856 as an alternative. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
4 products for Alternative Product
Target data
Function
Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain. (Microbial infection) Fatty acid synthetase activity is required for SARS coronavirus-2/SARS-CoV-2 replication.
Alternative names
FAS, FASN, Fatty acid synthase, Type I fatty acid synthase
Recommended products
Rabbit Recombinant Monoclonal Fatty Acid Synthase antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR7466
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1.34 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab221934 is the carrier-free version of Anti-Fatty Acid Synthase antibody [EPR7466] ab128870.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Immunoprecipitation - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--immunoprecipitation-img169715.jpg "Immunoprecipitation - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)")
Immunoprecipitation - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)
Purified Anti-Fatty Acid Synthase antibody [EPR7466] ab128870 at 1:30 dilution (2μg) immunoprecipitating Fatty Acid Synthase in HEK-293 whole cell lysate.
Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10 μg
Lane 2 (+): ab221934 + HEK-293 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Fatty Acid Synthase antibody [EPR7466] ab128870 in HEK-293 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) (1:10,000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: kDaThis data was developed using Anti-Fatty Acid Synthase antibody [EPR7466] ab128870, the same antibody clone in a different buffer formulation.
All lanes: Immunoprecipitation - Anti-Fatty Acid Synthase antibody [EPR7466] (Anti-Fatty Acid Synthase antibody [EPR7466] ab128870)
Predicted band size: 273 kDa
![Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--western-blot-img165657.jpg "Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)")
Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)
This data was developed using Anti-Fatty Acid Synthase antibody [EPR7466] ab128870, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] (Anti-Fatty Acid Synthase antibody [EPR7466] ab128870) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: Mouse liver lysate at 20 µg
Lane 4: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg
Lane 5: Mouse brain lysate at 20 µg
Lane 6: L6 (Rat skeletal muscle myoblast) whole cell lysate at 20 µg
Lane 7: Rat brain lysate at 20 µg
Lane 8: Rat liver lysate at 20 µg
Lane 9: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 273 kDa
![Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--flow-cytometry-intracellular-img169261.jpg "Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)")
Flow Cytometry (Intracellular) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)
Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labelling Fatty Acid Synthase with Purified Anti-Fatty Acid Synthase antibody [EPR7466] ab128870 at 1:50 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
This data was developed using Anti-Fatty Acid Synthase antibody [EPR7466] ab128870, the same antibody clone in a different buffer formulation.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img180914.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling Fatty Acid Synthase with Purified Anti-Fatty Acid Synthase antibody [EPR7466] ab128870 at 1:450 dilution (1.09 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using Anti-Fatty Acid Synthase antibody [EPR7466] ab128870, the same antibody clone in a different buffer formulation.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img171868.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue sections labeling Fatty Acid Synthase with Purified Anti-Fatty Acid Synthase antibody [EPR7466] ab128870 at 1:450 dilution (1.09 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using Anti-Fatty Acid Synthase antibody [EPR7466] ab128870, the same antibody clone in a different buffer formulation.
![Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--immunocytochemistry-immunofluorescence-img171138.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)")
Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)
Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Fatty Acid Synthase with Purified Anti-Fatty Acid Synthase antibody [EPR7466] ab128870 at 1:50 dilution (10 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using Anti-Fatty Acid Synthase antibody [EPR7466] ab128870, the same antibody clone in a different buffer formulation.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img182315.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling Fatty Acid Synthase with Purified Anti-Fatty Acid Synthase antibody [EPR7466] ab128870 at 1:450 dilution (1.09 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using Anti-Fatty Acid Synthase antibody [EPR7466] ab128870, the same antibody clone in a different buffer formulation.
![Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--western-blot-img169925.jpg "Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)")
Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Fatty Acid Synthase knockout HAP1 cell lysate (20 μg)
Lane 3: A549 cell lysate (20 μg)
Lane 4: Hu liver tissue lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-Fatty Acid Synthase antibody [EPR7466] ab128870 observed at 250 kDa. Red - loading control, ab18058, observed at 124 kDa.Anti-Fatty Acid Synthase antibody [EPR7466] ab128870 was shown to react with Fatty Acid Synthase in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when Fatty Acid Synthase knockout samples were examined. Wild-type and Fatty Acid Synthase knockout samples were subjected to SDS-PAGE. Anti-Fatty Acid Synthase antibody [EPR7466] ab128870 and ab18058 (loading control to Vinculin) were both diluted at 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
This image was produced using Anti-Fatty Acid Synthase antibody [EPR7466] ab128870, the same clone but in a different formulation.
All lanes: Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)
Predicted band size: 273 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--immunocytochemistry-immunofluorescence-img409145.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)")
Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)
This data was developed using Anti-Fatty Acid Synthase antibody [EPR7466] ab128870, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling with Anti-Fatty Acid Synthase antibody [EPR7466] ab128870 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cells is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at 1/1000 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--immunocytochemistry-immunofluorescence-img171138.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img182315.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)")
![Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--western-blot-img169925.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)")
![Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934), expandable thumbnail](https://content.abcam.com/products/images/fatty-acid-synthase-antibody-epr7466-bsa-and-azide-free-ab221934--immunocytochemistry-immunofluorescence-img409145.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Fatty Acid Synthase antibody [EPR7466] - BSA and Azide free (ab221934)")
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