Rabbit Polyclonal UBIM antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Ubiquitin-like protein FUBI.
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- Cell death & disease 14:7802023Investigation into the role of the MITA-TRIM38 interaction in regulating pyroptosis and maintaining immune tolerance at the maternal-fetal interface.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Liu,Yan Deng,An Wang,Bowen Liu,Xi Zhou,Tailang Yin,Yan Wang,Tao Tang,Yang Qiu,Jiao Chen,Jing YangPubMed 38012139
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human Ubiquitin-like protein FUBI. Database link P35544
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200 - 1/500 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/200 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Alternative names
Ubiquitin-like protein FUBI, FAU
Recommended products
Rabbit Polyclonal UBIM antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Ubiquitin-like protein FUBI.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human Ubiquitin-like protein FUBI. Database link P35544
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FAU protein also known as 40S ribosomal protein S30 plays an important role in the formation of ribosomes which are essential for protein synthesis. With a molecular mass of approximately 13 kDa it contributes to the small subunit of the ribosome assembly. This protein is expressed in several tissues including the liver kidney and pancreas indicating its ubiquitous role in maintaining cellular machinery. The FAU gene encodes this protein and its expression shows variation depending on tissue type and physiological conditions.
- Biological function summary
FAU / 40S ribosomal protein S30 is a component of the eukaryotic ribosomal 40S subunit participating in the translation process. It forms part of the ribosome complex with other ribosomal proteins and RNA which together drive the synthesis of proteins in cells by translating mRNA. Its role in ribosome assembly emphasizes its involvement in the overall cellular function of protein production which is fundamental for cell growth and division.
- Pathways
FAU protein integrates mainly within the process of translational initiation. The process includes various steps where the protein joins other ribosomal proteins and initiation factors to form functional ribosomes that start the translation of mRNA. FAU's interactions include associations with proteins like eIF3 an essential initiation factor which facilitates the accurate translation start site selection. This engagement ensures that FAU is integral to both the regulation and efficiency of protein synthesis a critical cellular activity.
- Associated diseases and disorders
FAU / 40S ribosomal protein S30 connects with certain cancers reflecting its importance in cell proliferation. Changes in FAU expression levels associate with malignant transformation particularly in liver cancer suggesting its potential role in tumor development. It also interacts with P53 a well-known tumor suppressor protein highlighting its relevance in apoptosis and cancer-related pathways. Additionally its altered regulation associates with ribosomopathies which are disorders arising from defects in ribosomal function underlining its essential contributions to cellular homeostasis and health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAU / 40S ribosomal protein S30 antibody (ab239073)
Paraffin-embedded human liver cancer tissue stained for FAU / 40S ribosomal protein S30 with ab239073 at a 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunocytochemistry/ Immunofluorescence - Anti-FAU / 40S ribosomal protein S30 antibody (ab239073)
A549 (Human lung carcinoma cell line) cells stained for FAU / 40S ribosomal protein S30 using ab239073 at a dilution of 1/66 in ICC/IF.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
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