JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB58881

Anti-FBH1 antibody [2353C1a]

Be the first to review this product! Submit a review

|

(5 Publications)

Mouse Monoclonal FBH1 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FBH1.

View Alternative Names

FBX18, FBXO18, FBH1, F-box DNA helicase 1, hFBH1, DNA 3'-5' helicase 1, F-box only protein 18

1 Images
Western blot - Anti-FBH1 antibody [2353C1a] (AB58881)
  • WB

Unknown

Western blot - Anti-FBH1 antibody [2353C1a] (AB58881)

The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

All lanes:

Western blot - Anti-FBH1 antibody [2353C1a] (ab58881)

All lanes:

immunizing recombinant fragment

Predicted band size: 118 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2353C1a

Isotype

IgG1

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human FBH1. The exact immunogen used to generate this antibody is proprietary information.

Q8NFZ0

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FBH1 also known as F-box helicase 1 is a DNA helicase with a molecular mass of about 120 kDa. It possesses ATPase activity and plays a role in unwinding DNA during replication and repair. FBH1 often localizes to the cell nucleus and shows expression in a variety of human tissues. Scientists also recognize it for its role in maintaining genome integrity particularly during the S-phase of the cell cycle.
Biological function summary

FBH1 contributes to the regulation of DNA replication and repair processes. It functions in tandem with other proteins to form a complex that can identify and repair damaged DNA strands. This protein complex is involved in maintaining genomic stability by preventing replication forks from collapsing and assisting in the resolution of DNA supercoiling and entanglements. FBH1 also links to the cellular response to double-strand breaks by promoting homologous recombination repair.

Pathways

FBH1 is closely involved in homologous recombination and DNA damage response pathways. It interacts with proteins such as RAD51 a homologous recombination repair protein and BRCA1 which plays a role in maintaining genomic stability. Through these interactions FBH1 ensures proper DNA repair and cell cycle progression which are critical for preventing genomic instability and tumorigenesis.

FBH1 has a notable connection to cancer. Dysregulation of FBH1 may lead to uncontrolled cell division and genomic instability contributing to the development of cancer. Moreover studies have shown that FBH1 interacts with the tumor suppressor protein p53 which plays a critical role in controlling cell growth and apoptosis. Understanding FBH1’s function and its interactions with proteins like p53 could provide insights into therapeutic targets for cancer treatment.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

3'-5' DNA helicase and substrate-recognition component of the SCF(FBH1) E3 ubiquitin ligase complex that plays a key role in response to stalled/damaged replication forks (PubMed : 11956208, PubMed : 23393192). Involved in genome maintenance by acting as an anti-recombinogenic helicase and preventing extensive strand exchange during homologous recombination : promotes RAD51 filament dissolution from stalled forks, thereby inhibiting homologous recombination and preventing excessive recombination (PubMed : 17724085, PubMed : 19736316). Also promotes cell death and DNA double-strand breakage in response to replication stress : together with MUS81, promotes the endonucleolytic DNA cleavage following prolonged replication stress via its helicase activity, possibly to eliminate cells with excessive replication stress (PubMed : 23319600, PubMed : 23361013). Plays a major role in remodeling of stalled DNA forks by catalyzing fork regression, in which the fork reverses and the two nascent DNA strands anneal (PubMed : 25772361). In addition to the helicase activity, also acts as the substrate-recognition component of the SCF(FBH1) E3 ubiquitin ligase complex, a complex that mediates ubiquitination of RAD51, leading to regulate RAD51 subcellular location (PubMed : 25585578).
See full target information FBH1
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

DNA repair 133:103611 PubMed38103522

2023

FBH1 deficiency sensitizes cells to WEE1 inhibition by promoting mitotic catastrophe.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy Jennings,Heather Andrews Walters,Tyler J McCraw,Joshua L Turner,Jennifer M Mason

Nature communications 14:3024 PubMed37230987

2023

Bre1/RNF20 promotes Rad51-mediated strand exchange and antagonizes the Srs2/FBH1 helicases.

Applications

Unspecified application

Species

Unspecified reactive species

Guangxue Liu,Jimin Li,Boxue He,Jiaqi Yan,Jingyu Zhao,Xuejie Wang,Xiaocong Zhao,Jingyan Xu,Yeyao Wu,Simin Zhang,Xiaoli Gan,Chun Zhou,Xiangpan Li,Xinghua Zhang,Xuefeng Chen

Signal transduction and targeted therapy 6:129 PubMed33785736

2021

PUMA facilitates EMI1-promoted cytoplasmic Rad51 ubiquitination and inhibits DNA repair in stem and progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Wook Kang,Zhiyan Zhan,Guangzhen Ji,Youzhou Sang,Daohong Zhou,Yanxin Li,Haizhong Feng,Tao Cheng

Nature communications 10:4410 PubMed31562309

2019

Non-enzymatic roles of human RAD51 at stalled replication forks.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer M Mason,Yuen-Ling Chan,Ralph W Weichselbaum,Douglas K Bishop

Nature communications 9:746 PubMed29467415

2018

PARP1 and PARP2 stabilise replication forks at base excision repair intermediates through Fbh1-dependent Rad51 regulation.

Applications

Unspecified application

Species

Unspecified reactive species

George E Ronson,Ann Liza Piberger,Martin R Higgs,Anna L Olsen,Grant S Stewart,Peter J McHugh,Eva Petermann,Nicholas D Lakin
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB133534

Anti-Rad51 antibody [EPR4030(3)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rad51 antibody [EPR4030(3)] (AB133534)

  • 4
  • 15
Primary Antibodies

AB81299

Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB81299)

  • 4
  • 17
Primary Antibodies

AB58416

Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E3 SUMO-protein ligase PIAS4/PIASy antibody (AB58416)

  • 0
  • 0
Primary Antibodies

AB192369

Anti-PIF1 antibody

Western blot - Anti-PIF1 antibody (AB192369)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com